Description: Mouse primary cardiac fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse primary cardiac fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 0.5 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

Description: C57BL/6 Mouse Primary Mammary Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Mammary Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Mammary Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Description: C57BL/6 Mouse Primary Ovarian Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Ovarian Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Ovarian Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Description: Mouse splenic fibroblasts are isolated from tissue of pathogen-free laboratory mice. Mouse splenic fibroblasts are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 0.5 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

Description: Mouse Embryonic Fibroblasts (MEFC) are isolated from day 13 mouse embryos of pathogen-free laboratory mice. They are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 0.5 hour and incubated in Culture Complete Growth Medium for 3-7 days.

Description: C57BL/6 Mouse Primary Lymphatic Fibroblasts from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Lymphatic Fibroblasts are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and is delivered frozen.Mouse Primary Lymphatic Fibroblasts are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of marker using the antibody of anti-FSP1/S100A4 by immunofluorescence staining. Cells can be expanded for 3-5 passages at a split ratio of 1:2 under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.Standard biochemical procedures performed with cell cultures include the assay of cell to cell interaction, RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Description: Mouse Peritoneal Macrophages are derived from the thioglycollate-elicited peritoneal cavity of pathogen-free laboratory adult mice. Mouse Peritoneal Macrophages are negative for bacteria, yeast, fungi, and mycoplasma. Each vial contains at least 8 x10^6 cells and is delivered frozen. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended. Mouse Peritoneal Macrophages are tested for expression of markers using antibodies, CD11b by flow cytometry. Standard biochemical procedures performed with cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining, flow cytometry or generating cell derivatives for desired research applications.

Description: C57BL/6 Mouse Primary Epidermal Keratinocytes from Gentaur are isolated from skin tissue of pathogen-free laboratory mice. C57BL/6 Mouse Primary Epidermal Keratinocytes are grown in a T25 tissue culture flask pre-coated with gelatin-based coating solution for 2 min and incubated in Gentaur's Culture Complete Growth Medium for 3-5 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5x106 cells per ml and is delivered frozen. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. The method we use to isolate primary epithelial cells was developed based on a combination of established and our proprietary methods. These cells are pre-coated with EpCAM-1 (CD326) antibody, followed by the application of magnetic beads pre-coated with secondary antibody.

Description: Mouse tail cells are isolated from day-1 neonatal tissue of pathogen-free laboratory mice. Mouse tail cells are mixed population of mouse spleen cells grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 0.5 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

Description: C57BL/6 Mouse Liver Kupffer Cells are derived from the liver of pathogen-free laboratory adult mice. Cells are grown in tissue culture flasks and incubated in Gentaur's Cell Culture Medium for 2-5 days. Prior to shipping, cells at passage 0 are detached from flasks and cryopreserved in vials. Each vial contains 3 x10^6cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells are tested for expression of markers using antibodies, CD11b by flow cytometry. Cells can be expanded on a multiwell culture plate ready for experiments under the cell culture conditions specified by Gentaur. Repeated freezing and thawing of cells is not recommended.

Description: C57BL/6 Mouse Bone Marrow Monocytes are derived from the tibias of pathogen-free laboratory adult mice. Each vial contains 10 x10^6 cells and is delivered frozen. Cells are negative for bacteria, yeast, fungi, and mycoplasma. Cells can be used for desired experiments and can not be passaged. Repeated freezing and thawing of cells is not recommended.

Description: Mouse bone marrow leukocytes (MBMLC) are derived from the tibias and femurs of pathogen-free laboratory adult mice. MBMLC are mixed population of mouse bone marrow cells. Each vial contains at least 10x10^6 cells and is delivered frozen.

Description: C57BL/6 Mouse Primary Liver Epithelial Cells from Gentaur are isolated from tissue of pathogen-free laboratory mice. Mouse Primary Liver Epithelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based coating solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium for 3-7 days. Cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 0.5×10^6 cells per ml and is delivered frozen.Mouse Primary Liver Epithelial Cells can be used in assays of cell to cell adhesion and migration. Standard biochemical procedures performed with epithelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.

Description: C57BL/6 mouse lung endothelial cells (MLUEC) are isolated from tissue of pathogen-free laboratory mice. C57BL/6 mouse lung endothelial cells are grown in T75 tissue culture flasks pre-coated with 0.2% gelatin for 0.5 hour and incubated in Culture Complete Growth Medium generally for 3-7 days.

Description: C57BL/6 Mouse Vein Endothelial Cells from Gentaur are isolated from inferior vena cava tissue of pathogen-free laboratory mice. Mouse Vein Endothelial Cells are grown in T25 tissue culture flasks pre-coated with gelatin-based solution for 0.5 hour and incubated in Gentaur's Culture Complete Growth Medium generally for 3-7 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x10^6 cells per ml and are delivered frozen.Mouse Vein Endothelial Cells can be used in assays of cell to cell adhesion, migration, vascular tube formation, or transendothelial resistance (TER). Standard biochemical procedures performed with endothelial cell cultures include RT-PCR, Western blotting, immunoprecipitation, immunofluorescent staining or immunofluorescent flow cytometry or generating cell derivatives for desired research applications.