Development of a quantitative validation method for forensic investigation of human spermatozoa using a commercial fluorescence staining kit (SPERM HY-LITER™ Express).
In the investigation of sexual assault, as well as in identifying the suspects, the detection of human sperm is important. More recently, a fluorescent staining kit for human spermatozoa, SPERM HY-LITER ™, has become available. This kit allows for microscopic observation of human sperm head uses antibodies tagged with fluorescent dyes. This kit is specific to human sperm and provide easy detection by luminescence.
However, the criteria should be established to objectively evaluate the fluorescent signals and to evaluate the efficiency of the coloring kit. These criteria will be indispensable for the investigation of forensic samples. In this study, the kit SPERM HY-LITER ™ Express, which is an improved version of the SPERM HY-LITER ™, are evaluated using image analysis procedures using Laplacian and Gaussian methods.
This method can be used to automatically select the important areas of the fluorescence generated by sperm. Fluorescence staining was evaluated and compared performance in a variety of experimental conditions, such as for traces of age and in combination with other chemical staining method. The morphological characteristics of human sperm is inserted into the criteria for the identification of sperm destination, based on the features measured from the point of neon. Using the criteria, non-specific or fluorescent spots are not significantly removed, and the specificity of the kit for human sperm confirmed. Image analysis methods and criteria established in this study are universal and can be applied in all experimental conditions. These criteria will improve the reliability of the judgment of the operator in the analysis of human sperm in a forensic sample.
Golgi-Cox method has become one of the most effective techniques to study the morphology of neuronal dendrites and dendritic spines. However, the reliability and time-consuming process of Golgi-Cox staining has been the main obstacle to the widespread application of this technique. To overcome these shortcomings and to promote this technique is very valuable, we develop fast FD GolgiStain ™ Kit is based on the principle of the method described by Ramón-Moliner in 1970 and Glaser and Van der Loos in 1981. The Kit significantly improves and simplifies the Golgi technique – Cox. This kit can be relied upon to visualize the morphological details of neurons, allowing for analysis of various parameters-such as dendritic morphology as dendritic length and branching pattern and dendritic spine number, shape, and size-both animal and human brain postmortem.
Development of a quantitative validation method for forensic investigation of human spermatozoa using a commercial fluorescence staining kit (SPERM HY-LITER™ Express).
Ki67 / double KIT immunohistochemical staining on skin mast cell tumors of dogs Boxer.
skin mast cell tumors (MCT) is the most frequent malignant tumor in dogs and dog breed Boxer has a higher incidence of this disease. Ki67 staining and KIT staining is widely used to predict the natural behavior in dogs MCT but no previous study has evaluated the double staining of this protein as a prognostic factor. Based on the prediction of biological behavior in dogs MCT, the aim of this study was to determine the Ki67 proliferation index in KIT positive cells using double immunohistochemical staining techniques.
Description: A competitive ELISA for quantitative measurement of Human Collagen Type I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Collagen Type I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Human Collagen Type I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Collagen Type I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Collagen Type I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Collagen Type I in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type I (COL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type I (COL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type I (COL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type I (COL1) in serum, plasma, tissue homogenates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Collagen Type I (COL1) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Description: A sandwich ELISA kit for detection of Collagen Type I from Human in samples from blood, serum, plasma, cell culture fluid and other biological fluids.
Description: A sandwich ELISA for quantitative measurement of Human Collagen Type I Alpha 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Collagen Type I Alpha 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Human Collagen Type I Alpha 2 in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
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Sixty-nine MCT of Boxer dogs selected and constructed for immunohistochemistry tissue microarray double stained. Double positive (Ki67 (+) / KIT (+)) was observed in 20/69 (29%) MCT, with an average of 9.06 double positive cells per core network (range of 0.48% -43.97%) and Ki67 (-) / KIT (+) animals have a longer life time of Ki67 (+) / KIT (+) animals (p = 0.03).
