The prevention of foodborne diseases is one of the main objectives of the health authorities. For this purpose, analytical techniques for detecting and / or quantifying the microbiological contamination of food prior to release on the market are required. The management and control of pathogens of food origin have generally been based on conventional detection methodologies, which do not only consume a lot of time and labor, but also involve high consumer material costs. However, this management perspective has changed over time that the food industry requires effective analytical methods that achieve quick results.
This review covers the historical context of traditional methods and their passage in the latest developments of rapid methods and their implementation in the food sector. Improvements and limitations in the detection of the most relevant pathogens are discussed from a perspective applicable to the current situation in the food industry. Given the efforts and recent developments, fast and accurate methods already used in the food industry will also be affordable and portable and provide connectivity in the near future, which improves decision-making and safety throughout the chain Food. A retrospective epidemiological study describing the characteristics, the incidence rates (IR) and the microbiological etiology of the SCAP in Central Australia.
Adult Patients Admitted to Alice Springs Hospital Intensive Care Unit (ICU) between 2011-2014 which has been included the IDSA / ATS definition of the SCAP. Medical records have been examined and compared between Aboriginal and non-Aboriginal patients. Primary results were an incidence rate and microbiological etiology of SCAP. Secondary results were 30 days mortality and a residence time of the ICU and the hospital (LOS).
Plancostomycetes as bacteria associated with the host: a perspective that keeps the promise of their future isolates, imitating their aboriginal environmental niches in clinical microbiology laboratories
Traditionally recognized as environmental bacteria, plancostomycetes have recently been linked to human pathology as opportunistic pathogens, providing great interest to clinical microbiologists. However, the absence of appropriate culture media limits our future surveys because no plackctomyte has ever been isolated from patient specimens despite multiple attempts. Several plancostomycetes have no cultivable members and are recognized only by detecting and analyzing the sequence of the arrn genes. Cultivated representatives are tedious slow growth bacteria and most of the time culture on synthetic media.
As a result, the provision of environmental and nutritional conditions such as those existing in natural habitat in natural habitat where non-skin / refractory bacteria can be detected could be an option for their potential isolation. As a result, we have systematically examined the different natural plancostomycete habitats, to examine their nutritional requirements, the physicochemical characteristics of their natural ecological niches, the current methods of cultivation of plackcetes and gaps, from a perspective of data collection. to optimize the conditions and the culture protocols of these tedious bacteria.
Plancptomycetes are prevalent in freshwater, seawater and terrestrial environments, mainly associated with particles or organisms such as macroalgae, marine sponges and lichens, depending on the species and polysaccharides metabolizable by their sulfatasis. Most plancostomycetes are developing in poor nutrient oligotrophic environments with a pH ranging from 3.4 to 11, but some strains can also develop in media rich in nutrients such as M600 / M14. In addition, a variation in seasonality of abundance is observed and flowering occurs in the summer-early autumn, correlated with strong algae growth in marine environments. Most placalcètes are mesophilic, but with some plancostomycetes being thermophilic (50 ° C to 60 ° C).
From hazard analysis to risk control using rapid methods in microbiology: A practical approach for the food industry
Mini Review: Clinical Routine Microbiology in the Era of Digital Automation and Health
Clinical microbiology laboratories are the first line of infectious disease and antibiotic resistance, including new emerging. Although most clinical laboratories are still based on conventional methods, a cascade of technological change, driven by digital imaging and high-speed sequencing, will revolutionize clinical diagnostics management for direct detection of bacteria and susceptibility testing. rapid antimicrobial. IMPORTANT, such technological advances occur in the golden age of machines learning where computers do not act more passively in the mining of data, but once trained, can also help doctors take Decisions on the optimal diagnosis and administration of treatment.
The additional physical integration potential of new technologies in an automation chain, associated with the software to the automatic learning of data analyzes, is seduced and lead to faster management of infectious diseases. However, if, on the one hand, the technological advancement would have a better performance than conventional methods, on the other side, this evolution disputes clinicians in terms of data interpretation and impact on the whole of the Organization and management of the staff of the hospital.
Description: Histamine-α,α,β,β-d4 (dihydrochloride) is the deuterium labeled Histamine. Histamine is an organic nitrogenous compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter.
Description: SMARCA-BD ligand 1 for Protac dihydrochloride is a compound that binds to the BAF ATPase subunits SMARCA2, and used for degrading SMARCA2, based on PROTAC[1].
Description: MPP dihydrochloride is a potent and selective ER (estrogen receptor) modulator. MPP dihydrochloride induces significant apoptosis in the endometrial cancer and oLE cell lines. MPP dihydrochloride reverses the positive effects of beta-estradiol. MPP dihydrochloride has mixed agonist/antagonist action on murine uterine ERalpha in vivo[1][2][3].
Description: SAG dihydrochloride is a potent Smoothened (Smo) receptor agonist (EC50=3 nM; Kd=59 nM). SAG dihydrochloride activates the Hedgehog signaling pathway and counteracts Cyclopamine (HY-17024) inhibition of Smo[1][2][3].
Description: IT1t dihydrochloride is a potent antagonist of CXCR4 with IC50 value of 8.0 nM [1]. C-X-C chemokine receptor type 4 (CXCR4) is an ?-chemokine receptor for chemokine CXCL12.
In this mini-examination, we discuss such technological achievements offering practical examples of their operability, but also their limits and potential problems that their implementation could increase in clinical microbiology laboratories.
Gina
