Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Background: Following liver injury, mast cells (MC) migrate to the liver and is activated in patients with cholestasis. Inhibition of MC decreased mediator ductular reaction (DR) and liver fibrosis. TGF-β1 contributes to fibrosis and promote liver disease.


Objective: To demonstrate that MC induces reintroduction of cholestatic injury with TGF-β1.


Methods: Wild-type, KitW-sh (MC-deficient), and Mdr2 – / – mice lacking L-histidine decarboxylase injected with vehicle or PKH26-tagged murine MC by treatment with 0.01% DMSO or inhibitor of TGF-βR, LY2109761 (TGF-βRi, 10 M) three days before the bag. liver damage was assessed by H & E and serum chemistry. MC injected detected in the liver, spleen and lungs by immunofluorescence (IF). DR is measured by CK-19 immunohistochemistry (IHC) and F4 / 80 staining coupled with qPCR for IL-1β, IL-33 and F4 / 80; aging bile evaluated by IF or qPCR for p16, p18 and p21. Fibrosis was evaluated by Sirius Red / Fast Green staining and IF to SYP 9, desmin, and α-SMA. secretion of TGF-β1 / expression was measured by EIA and qPCR. Angiogenesis is detected by the IF to vonWillebrand Factor and VEGF-C qPCR. In vitro, MC TGF-β1 expression / secretion was measured after treatment of TGF-βRi; conditioned media is collected. Cholangiocytes and hepatic stellate cell (HSCs) were treated with conditioned medium and bile MC proliferation / aging is measured by MTS and qPCR; HSC activation was evaluated for α-SMA, SYP-9 and expression of collagen type-1a.


Results: MC injection recapitulation cholestatic liver injury is characterized by an increase in DR; fibrosis / TGF-β1 secretion; and angiogenesis. Injection MC-TGF-βRi reverse this parameter. In vitro, induces gall MC proliferation / activation of HSC aging and overturned by MC less TGF-β1.


Conclusion: Our new research shows that the reintroduction of cholestatic liver injury mimics MC and MC derived TGF-β1 may be a target in chronic cholestatic liver disease.

 Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling
Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Relieves trimetazidine hypoxia / reoxygenation-induced apoptosis in neonatal rat cardiomyocytes through up-regulating the expression of HMGB1 to promote autophagy

Previous studies have shown the effects of trimetazidine (TMZ) on reducing cardiomyocyte hypoxia / reoxygenation (H / R) injury and the protective effect of autophagy on Ischemia-Reperfusion (I / R) injury to cells. However, whether TMZ protection mechanisms are also involved in autophagy remains unclear.

Our study introduces the role of HMGB1 to check TMZ on autophagy regulation on cardiomyocyte injury H / R. After cell extraction and identification by anti-α-actin staining, cardiomyocytes created hypoxia and reoxygenated, each for 3 hours, and then treated with various concentrations TMZ and transfected with siHMGB1. cell viability and apoptosis were measured by MTS method and flow cytometry, respectively. Autophagy- related factor expression (LC3-I, LC3-II, Beclin-1) and HMGB1 was detected by Western blot and qPCR. Lactate dehydrogenase (LDH) release was assessed by ELISA kit. Cardiomyocytes extracted. H / R lowering of cell viability and increase the level of LDH and cardiomyocyte apoptosis. TMZ had no effect on cardiomyocytes not treated, but reversed the adverse effects of H / R in cardiomyocytes.

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 276

?-Galactosidase Staining Kit

AKR-100 1 kit
EUR 518.4
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 444
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 276

Human Histochemistry Staining Kit

20-abx299039
  • EUR 1045.20
  • EUR 1462.80
  • EUR 678.00
  • 150 tests
  • 500 tests
  • 50 tests

Beta-Galactosidase Staining Kit

K802-250
EUR 352.8

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 351.6

Silver benzoat

20-abx184359
  • EUR 309.60
  • EUR 427.20
  • EUR 276.00
  • 10 g
  • 25 g
  • 5 g

Silver trifluoromethanesulfonate

20-abx184360
  • EUR 661.20
  • EUR 343.20
  • 100 g
  • 25 g

Silver carbonate

20-abx184771
  • EUR 526.80
  • EUR 292.80
  • 100 g
  • 25 g

Silver Phosphate

abx188878-500g 500 g
EUR 1195.2

Silver stearate

GK5303-10G 10 g
EUR 435.6

Silver stearate

GK5303-1G 1 g
EUR 112.8

Silver stearate

GK5303-5G 5 g
EUR 265.2

Silver nitrate

GK7224-100G 100 g
EUR 265.2

Silver nitrate

GK7224-10G 10 g
EUR 84

Silver nitrate

GK7224-250G 250 g
EUR 447.6

Silver nitrate

GK7224-25G 25 g
EUR 112.8

Silver nitrate

GK7224-50G 50 g
EUR 170.4

Silver nitrate

GK7224-5G 5 g
EUR 67.2

Silver sulfate

GK0139-100G 100 g
EUR 361.2

Silver sulfate

GK0139-25G 25 g
EUR 165.6

Silver sulfadiazine

GK2380-10G 10 g
EUR 93.6

Silver sulfadiazine

GK2380-25G 25 g
EUR 151.2

Silver sulfadiazine

GK2380-5G 5 g
EUR 70.8

isa silver

ISA34 ea
EUR 109.2

Silver methanesulfonate

GX5020-25G 25 g
EUR 208.8

Silver methanesulfonate

GX5020-5G 5 g
EUR 85.2

Silver nitrate

SB0479 25g
EUR 170.66

Frit Kit

FRIT-KIT 1each
EUR 148.8
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Sterling Rapid Silver Stain Kit

NAT1106 1KIT
EUR 231.42

Nerve Terminal Staining Kit V

70034 1SET
EUR 610.8
Description: Minimum order quantity: 1 unit of 1SET

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 355.2
Description: Caspase Staining Kit for use activity in the research laboratory

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 765.6
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 314.4

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 494.4

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 314.4

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 552

DNA Ploidy Analysis Staining Kit

K1439-1
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5
EUR 2402.4

Fluorescein Active Caspase Staining Kit

K2047-100 100 assays
EUR 686.4

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 285.6

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 686.4

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 285.6

Live-Dead Cell Staining Kit

K501-100
EUR 561.6

Column Packing Kit

PACK-KIT 1pack
EUR 1242
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 319.2

Silver Colloid (20nm)

63R-SC001 100 ml
EUR 613.2
Description: 20nm colloidal silver particles

Silver homologue Antibody

abx430963-200ul 200 ul
EUR 460.8

Silver P-Toluenesulfonate

20-abx186559
  • EUR 526.80
  • EUR 276.00
  • 25 g
  • 5 g

calibration solution silver

ISC34 ea
EUR 109.2

silver/sulphide electrode

ISE34B ea
EUR 392.4

silver electrode, s7

SO65X ea
EUR 210

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1332

Pesticide Kit Containing All 10 Multi-Compound Mixes

SPXPR-KIT 1ML
EUR 1923.18

CaspGLOW Active Caspase 12 Staining Kit

55R-1291 25 assays
EUR 338.4
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit

55R-1293 25 assays
EUR 340.8
Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit

55R-1294 25 assays
EUR 355.2
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit

55R-1295 25 assays
EUR 355.2
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 9 Staining Kit

55R-1296 25 assays
EUR 355.2
Description: Caspase 9 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase Staining Kit

55R-1297 25 assays
EUR 355.2
Description: Caspase Staining Kit for use activity in the research laboratory

Yeast Viability Staining Kit (Green/Red)

31063-1 1kit
EUR 566.4
Description: Minimum order quantity: 1 unit of 1kit

Human Type I Collagen Staining Kit

9044 1 kit
EUR 406.26
Description: Human Type I Collagen Staining Kit

Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
EUR 226.8

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 292.8

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 249.6

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 153.6

StemTAG Alkaline Phosphatase Staining Kit (Red)

CBA-300 100 assays
EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

StemTAG Alkaline Phosphatase Staining Kit (Purple)

CBA-306 100 assays
EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Human FoxP3 Transcription Factor Staining Kit

OKTA00001 KIT
EUR 544.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Human FoxP3 Transcription Factor Staining Kit

OKTA00004 KIT
EUR 544.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Gel-FAST? Gel Staining/Destaining Kit

K901-40
EUR 210

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-100
EUR 660

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-25
EUR 280.8

Genorise® Alkaline Phosphatase Staining Kit

GR107045 100 tests
EUR 346.8

Fluorescein Active Caspase-12 Staining Kit

K2046-100 100 assays
EUR 686.4

Fluorescein Active Caspase-12 Staining Kit

K2046-25 25 assays
EUR 285.6

Fluorescein Active Caspase-2 Staining Kit

K2048-100 100 assays
EUR 686.4

Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
EUR 285.6

Fluorescein Active Caspase-3 Staining Kit

K2049-100 100 assays
EUR 686.4

Fluorescein Active Caspase-3 Staining Kit

K2049-25 25 assays
EUR 285.6

Fluorescein Active Caspase-8 Staining Kit

K2050-100 100 assays
EUR 686.4

Fluorescein Active Caspase-8 Staining Kit

K2050-25 25 assays
EUR 285.6

Fluorescein Active Caspase-9 Staining Kit

K2051-100 100 assays
EUR 686.4

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 285.6

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 686.4

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 285.6

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 686.4

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 285.6

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 686.4

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 285.6

Lipid (Oil Red O) staining Kit

K580-24
EUR 542.4

CaspGLOW? Red Active Caspase Staining Kit

K190-100
EUR 627.6

CaspGLOW? Red Active Caspase Staining Kit

K190-25
EUR 288

Stayright™ Purple HRP Staining Kit

45905 5 mL
EUR 109

Stayright™ Purple HRP Staining Kit

45906 50 mL
EUR 334

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1358.4

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1358.4

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1358.4

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 966

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1358.4

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1358.4

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1358.4

AE-1360 EzStain silver

2332360 2unit
EUR 396
Description: Si l ver for Pr ot ei n& DN A

2,4-Pentanedione, Silver Derivative

20-abx186186
  • EUR 360.00
  • EUR 1528.80
  • EUR 693.60
  • 1 g
  • 25 g
  • 5 g

Competent Cells, Silver Efficiency

BIO-85026 2ml ( 10 x 200µl ) Ask for price

double silver electrode, s7

SP05X ea
EUR 279.6

Silver Nitrate Solution (0.2%)

SNS125 125 ml
EUR 86.4

Silver Nitrate Solution (0.2%)

SNS500 500 ml
EUR 142.8

Silver Nitrate Solution (0.2%)

SNS999 1000 ml
EUR 208.8

Silver Nitrate Solution (5%)

SNV125 125 ml
EUR 121.2

Silver Nitrate Solution (5%)

SNV500 500 ml
EUR 277.2

Silver Nitrate Solution (10%)

SNX009 9 ml
EUR 73.2

Silver Nitrate Solution (10%)

SNX065 65 ml
EUR 124.8

Silver Nitrate Solution (10%)

SNX125 125 ml
EUR 178.8

Silver Nitrate Solution (10%)

SNX500 500 ml
EUR 414

Silver Nitrate Solution (1%)

SNY125 125 ml
EUR 121.2

Silver Nitrate Solution (1%)

SNY500 500 ml
EUR 206.4

Volumetric Silver Nitrate 0.05M

N20050W 2.5L
EUR 135.66

Silver Anodes for CHL2000

CHL2020 PK3
EUR 80.94

Electrode chlorided silver senso

TIT3127 EACH
EUR 478.8

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 183.6

PI / RNase Staining Buffer

20-abx090619
  • EUR 326.40
  • EUR 276.00
  • 100 tests
  • 50 tests

Ponceau S Staining Solution

B7778-100000 100 g
EUR 195.6

Ponceau S Staining Solution

B7778-50000 50 g
EUR 151.2

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 211.2

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 69.6

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

Ponceau S Staining Solution

PW005 100ml
EUR 82.97

Disposable Slide Staining Trays

MIC5070 PK4
EUR 71.82

Staining Trough+Lid Rectangular

MIC6008 EACH
EUR 23.03

Nalgene Box Staining 13x13x5cm

BOX1040 EACH
EUR 42.18

Claritas PPT Grade Minerals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXMT-KIT 30ML
EUR 237.12

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 5929.2

Claritas PPT Grade Heavy Metals Testing Kit for ICP-MS 1000 ug/mL (1000 ppm) 6 each 30mL

SPXHM-KIT 30ML
EUR 245.1

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 5929.2

CaspGLOW Red Active Caspase 3 Staining Kit

55R-1298 25 assays
EUR 355.2
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 8 Staining Kit

55R-1299 25 assays
EUR 355.2
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 9 Staining Kit

55R-1300 25 assays
EUR 355.2
Description: Caspase 9 Staining Kit for use activity in the research laboratory

Sirius Red/Fast Green Collagen Staining Kit

9046 30-50 samples
EUR 164.4
Description: Sirius Red/Fast Green Collagen Staining Kit

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230 50 assays
EUR 622.8
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230-5 5 x 50 assays
EUR 2461.2
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Protein Stain-EZ J Phosphoprotein Staining Kit

BSP043 2x10minigel, 20prep
EUR 133.08

Blue Feulgen DNA Ploidy Analysis Staining Kit

DPK500 1 kit(s)
EUR 427.2

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-100
EUR 627.6

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-25
EUR 288

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-100
EUR 620.4

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-25
EUR 288

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-100
EUR 639.6

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-25
EUR 288

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-100
EUR 660

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-25
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-100
EUR 627.6

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-25
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-100
EUR 627.6

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-25
EUR 280.8

EZCell? Intracellular Zinc Staining Kit (Cell-Based)

K525-50
EUR 679.2

Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based)

K458-50
EUR 686.4

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

K449-50
EUR 652.8

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-100
EUR 660

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-25
EUR 288

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-100
EUR 660

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-25
EUR 288

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2558.4

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2558.4

Silver Nitrate Solution (2%), Acidulated

SNA030 30 ml
EUR 105.6

Silver Nitrate Solution (2%), Acidulated

SNA500 500 ml
EUR 363.6

Silver Nitrate Solution (0.5%), Acidulated

SNB125 125 ml
EUR 117.6

Silver Nitrate Solution (0.5%), Acidulated

SNB500 500 ml
EUR 277.2

silver electrode, dj, 1m, bnc

SO66B ea
EUR 256.8

EP Reagent 0.1M Silver Nitrate

3005600 1L
EUR 132.24

EP Reagent 0.001M Silver Nitrate

3009300 1L
EUR 167.58

Silver Oxide Button Cell SR44

BAT8023 PK10
EUR 48.34

Cell Button Silver Oxide SR41

BAT9040 EACH
EUR 2.96

Cell Button Silver Oxide SR43

BAT9042 EACH
EUR 10.26

Cell Button Silver Oxide SR48

BAT90480 PK10
EUR 29.64

CP 0.1M Silver Nitrate Solution

CP1011 1L
EUR 115.14

Std Clamp Rot Microtome Silver

HIS3024 EACH
EUR 554.04

Uni Clamp Rot Microtome Silver

HIS3026 EACH
EUR 644.1

PROGARD SILVER 1 PRETREATMENT PACK

PR0G000S1 EACH
EUR 290.7

The expression of LC3-II, Beclin-1, and HMGB1 and the ratio of LC3-II / LC3-I increased in H / cardiomyocytes R-processed and subsequently appointed by TMZ pretreatment. However, transfection siHMGB1 exacerbate the effects of H / R in cardiomyocytes and suppresses the protective effects of TMZ on the H / R defective cardiomyocytes with increasing levels of LDH and apoptosis and reduced survival of cardiomyocytes.

Leave A Comment