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Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Background: Following liver injury, mast cells (MC) migrate to the liver and is activated in patients with cholestasis. Inhibition of MC decreased mediator ductular reaction (DR) and liver fibrosis. TGF-β1 contributes to fibrosis and promote liver disease.


Objective: To demonstrate that MC induces reintroduction of cholestatic injury with TGF-β1.


Methods: Wild-type, KitW-sh (MC-deficient), and Mdr2 – / – mice lacking L-histidine decarboxylase injected with vehicle or PKH26-tagged murine MC by treatment with 0.01% DMSO or inhibitor of TGF-βR, LY2109761 (TGF-βRi, 10 M) three days before the bag. liver damage was assessed by H & E and serum chemistry. MC injected detected in the liver, spleen and lungs by immunofluorescence (IF). DR is measured by CK-19 immunohistochemistry (IHC) and F4 / 80 staining coupled with qPCR for IL-1β, IL-33 and F4 / 80; aging bile evaluated by IF or qPCR for p16, p18 and p21. Fibrosis was evaluated by Sirius Red / Fast Green staining and IF to SYP 9, desmin, and α-SMA. secretion of TGF-β1 / expression was measured by EIA and qPCR. Angiogenesis is detected by the IF to vonWillebrand Factor and VEGF-C qPCR. In vitro, MC TGF-β1 expression / secretion was measured after treatment of TGF-βRi; conditioned media is collected. Cholangiocytes and hepatic stellate cell (HSCs) were treated with conditioned medium and bile MC proliferation / aging is measured by MTS and qPCR; HSC activation was evaluated for α-SMA, SYP-9 and expression of collagen type-1a.


Results: MC injection recapitulation cholestatic liver injury is characterized by an increase in DR; fibrosis / TGF-β1 secretion; and angiogenesis. Injection MC-TGF-βRi reverse this parameter. In vitro, induces gall MC proliferation / activation of HSC aging and overturned by MC less TGF-β1.


Conclusion: Our new research shows that the reintroduction of cholestatic liver injury mimics MC and MC derived TGF-β1 may be a target in chronic cholestatic liver disease.

Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling
Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Relieves trimetazidine hypoxia / reoxygenation-induced apoptosis in neonatal rat cardiomyocytes through up-regulating the expression of HMGB1 to promote autophagy

Previous studies have shown the effects of trimetazidine (TMZ) on reducing cardiomyocyte hypoxia / reoxygenation (H / R) injury and the protective effect of autophagy on Ischemia-Reperfusion (I / R) injury to cells. However, whether TMZ protection mechanisms are also involved in autophagy remains unclear.

Our study introduces the role of HMGB1 to check TMZ on autophagy regulation on cardiomyocyte injury H / R. After cell extraction and identification by anti-α-actin staining, cardiomyocytes created hypoxia and reoxygenated, each for 3 hours, and then treated with various concentrations TMZ and transfected with siHMGB1. cell viability and apoptosis were measured by MTS method and flow cytometry, respectively. Autophagy- related factor expression (LC3-I, LC3-II, Beclin-1) and HMGB1 was detected by Western blot and qPCR. Lactate dehydrogenase (LDH) release was assessed by ELISA kit. Cardiomyocytes extracted. H / R lowering of cell viability and increase the level of LDH and cardiomyocyte apoptosis. TMZ had no effect on cardiomyocytes not treated, but reversed the adverse effects of H / R in cardiomyocytes.

Prosi Silver Staining Kit for 25 mini gel
S3200-020 1kit
EUR 433.2

Silver protein for bodian staining 7.5-8.5% Ag
S03380 25G
EUR 545.62
Description: CAS N° 9008-42-8

SDS PAGE Kit (Teaching)
83775 15 expt. Kit
EUR 85.44
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DNA Ligation Kit (Teaching)
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HiPer® Protein Estimation Teaching Kit (
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EzWay Protein-Silver Staining Kit
K14040D 20A
EUR 188
Description: https://komabiotech.co.kr/labiskoma/datasheet/K14040D-korean.pdf

HiPer® Latex Agglutination Teaching Kit
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BioLit Gene Amplification Kit - PCR (Teaching)
19317 10 expt. Kit
EUR 104.17
Description: Part E

BioLit Gene Amplification Kit - PCR (Teaching)
19317-1 25 expt. Kit
EUR 147.88
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HiPer® Yeast Transformation Teaching Kit
HTBM025E-10PR 1 unit
EUR 62.08
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HTBM022E-5PR 1 unit
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HiPer® Bacterial RNA Teaching Kit (Colum
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HiPer® Cloning Teaching Kit (Blue-White
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Genomic DNA Extraction (from leaves) Kit (Teaching)
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EUR 89.29
Description: Part E

Genomic DNA Extraction (from bacteria) Kit (Teaching)
83462 15 expt. Kit
EUR 63.97
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Plasmid Isolation Kit from Bacterial cells (Teaching)
27725 15 expt. Kit
EUR 57.99
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Plasmid Isolation Kit from Bacterial cells (Teaching)
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EUR 78.68
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HiPer® Relative Quantification Teaching
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HiPer® Bacterial Conjugation Teaching Ki
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HiPer® Radial Immunodiffusion Teaching K
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HiPer® Antibiotic Sensitivity Teaching K
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HiPer® Antigen Capture ELISA Teaching Ki
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HiPer® Bacterial Growth Curve Teaching K
HTM001E-10PR 1 unit
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HiPer® Plasmid DNA Extraction Teaching K
HTBM002E-10PR 1 unit
EUR 38.51
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HiPer® Plasmid DNA Extraction Teaching K
HTBM002E-20PR 1 unit
EUR 55.13
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HiPer® Plasmid DNA Extraction Teaching K
HTBM003E-20PR 1 unit
EUR 65.04
Description: HiPer® Plasmid DNA Extraction Teaching K

SpinCol Plasmid Miniprep Kit From Bacterial cells (Teaching)
52166 20 expt. Kit
EUR 42.76
Description: Part E

SpinCol Plasmid Miniprep Kit From Bacterial cells (Teaching)
52166-1 50 expt. Kit
EUR 60.64
Description: Part E

Bacterial Transformation Kit – 2 (with blue white screening) (Teaching)
28660 15 expt. Kit
EUR 89.55
Description: Part E

Bacterial Transformation Kit – 2 (with blue white screening) (Teaching)
28660-1 30 expt. Kit
EUR 127.61
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Silver Stain kit
AR0171 1 kit (for 30 assays to stain the gel of 5 X8.5)
EUR 182.4

ElectroPure„¢ Silver Stain Kit
16717-1 1kit
EUR 392.04

HE Staining Kit
E-IR-R117-100mL 100mL
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PI Staining Kit
abx097208-1Kit 1 Kit
EUR 276

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MBS2567140-100mL 100mL
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HE staining Kit
MBS2567140-50mL 50mL
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HE staining Kit
MBS2567140-5x100mL 5x100mL
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PI Staining Kit
MBS826722-01mL 0.1mL
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PI Staining Kit
MBS826722-5x01mL 5x0.1mL
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DAPI Staining Kit
abx097206-100l 100 µl
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TRAP Staining Kit
KT-008 1 kit
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MBS826728-01mL 0.1mL
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Hoechst 33342 Staining Kit
abx097207-100l 100 µl
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Hoechst 33342 Staining Kit
MBS826727-01mL 0.1mL
EUR 140

Hoechst 33342 Staining Kit
MBS826727-5x01mL 5x0.1mL
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Sterling Rapid Silver Stain Kit - 1KIT
NAT1106 1KIT
EUR 337.5

Cell Cycle Staining Kit
KTA2020-100T 100 T
EUR 119
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit
KTA2020-50T 50 T
EUR 69
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Cell Cycle Staining Kit
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Cell Cycle Staining Kit
MBS9718793-100Tests 100Tests
EUR 225

Cell Cycle Staining Kit
MBS9718793-500Tests 500Tests
EUR 355

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EUR 195

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EUR 1580

Live NucRed staining kit
EGY069 >100T
EUR 210

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MBS8584964-100Tests 100Tests
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MBS8584964-5x100Tests 5x100Tests
EUR 1405

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EGY068 >100T
EUR 210

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EGY070 >100T
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MBS8584963-100Tests 100Tests
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MBS8584963-5x100Tests 5x100Tests
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MBS8584965-100Tests 100Tests
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Dead NucGreen staining kit
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Stains All Gel Staining Kit
KT-792 20 tests
EUR 737

CellSurface FLOW Staining Kit
MBS668926-1Kit 1Kit
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CellSurface FLOW Staining Kit
MBS668926-5x1Kit 5x1Kit
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β-Galactosidase Staining Kit
AKR-100 1 kit
EUR 288
Description: Excepted Quantities

AO/PI Double Staining Kit
K2238-100 100T
EUR 40
Description: Cell Biology|Cell Apoptosis

AO/PI Double Staining Kit
K2238-500 500T
EUR 120
Description: Cell Biology|Cell Apoptosis

Beta Galactosidase Staining Kit
55R-1541 250 assays
EUR 300
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Dual- Color AP Staining Kit
AP100D-1 100 Assays
EUR 494.4

Live/Dead Cell Staining Kit
55R-1434 100 tests
EUR 500
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Human Histochemistry Staining Kit
20-abx299039
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Live-Dead Cell Staining Kit
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Nerve Terminal Staining Kit I
70030-1 KT
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70034 1SET
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Nerve Terminal Staining Kit V
70034-1 KT
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Purple-Color AP Staining Kit
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70031-1 1SET
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70032-1 KT
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Red Active Caspase Staining Kit
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Red Active Caspase Staining Kit
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QUANTOM Total Cell Staining Kit
LBQ13501 each
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DNA Ploidy Analysis Staining Kit
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DNA Ploidy Analysis Staining Kit
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QUANTOM Viable Cell Staining Kit
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Stayrightâ„¢ Purple HRP Staining Kit
45905 5 mL
EUR 112
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit
45905-5mL 5 mL
EUR 109
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit
45906 50 mL
EUR 341
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit
45906-50mL 50 mL
EUR 334
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Lipid (Oil Red O) staining Kit
K580-24 each
EUR 542.4

Hematoxylin and Eosin Staining Kit
K1142-100 100mL
EUR 40
Description: Protein & Immunology|Immunofluorescence & Immunohistochemistry

Hematoxylin and Eosin Staining Kit
K1142-1000 2×500mL
EUR 62
Description: Hematoxylin and Eosin Staining，one of the most widely used staining technique in histopathology

Hematoxylin and Eosin Staining Kit
K1142-500 500mL
EUR 72
Description: Protein & Immunology|Immunofluorescence & Immunohistochemistry

The expression of LC3-II, Beclin-1, and HMGB1 and the ratio of LC3-II / LC3-I increased in H / cardiomyocytes R-processed and subsequently appointed by TMZ pretreatment. However, transfection siHMGB1 exacerbate the effects of H / R in cardiomyocytes and suppresses the protective effects of TMZ on the H / R defective cardiomyocytes with increasing levels of LDH and apoptosis and reduced survival of cardiomyocytes.

Gina

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