Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Background: Following liver injury, mast cells (MC) migrate to the liver and is activated in patients with cholestasis. Inhibition of MC decreased mediator ductular reaction (DR) and liver fibrosis. TGF-β1 contributes to fibrosis and promote liver disease.


Objective: To demonstrate that MC induces reintroduction of cholestatic injury with TGF-β1.


Methods: Wild-type, KitW-sh (MC-deficient), and Mdr2 – / – mice lacking L-histidine decarboxylase injected with vehicle or PKH26-tagged murine MC by treatment with 0.01% DMSO or inhibitor of TGF-βR, LY2109761 (TGF-βRi, 10 M) three days before the bag. liver damage was assessed by H & E and serum chemistry. MC injected detected in the liver, spleen and lungs by immunofluorescence (IF). DR is measured by CK-19 immunohistochemistry (IHC) and F4 / 80 staining coupled with qPCR for IL-1β, IL-33 and F4 / 80; aging bile evaluated by IF or qPCR for p16, p18 and p21. Fibrosis was evaluated by Sirius Red / Fast Green staining and IF to SYP 9, desmin, and α-SMA. secretion of TGF-β1 / expression was measured by EIA and qPCR. Angiogenesis is detected by the IF to vonWillebrand Factor and VEGF-C qPCR. In vitro, MC TGF-β1 expression / secretion was measured after treatment of TGF-βRi; conditioned media is collected. Cholangiocytes and hepatic stellate cell (HSCs) were treated with conditioned medium and bile MC proliferation / aging is measured by MTS and qPCR; HSC activation was evaluated for α-SMA, SYP-9 and expression of collagen type-1a.


Results: MC injection recapitulation cholestatic liver injury is characterized by an increase in DR; fibrosis / TGF-β1 secretion; and angiogenesis. Injection MC-TGF-βRi reverse this parameter. In vitro, induces gall MC proliferation / activation of HSC aging and overturned by MC less TGF-β1.


Conclusion: Our new research shows that the reintroduction of cholestatic liver injury mimics MC and MC derived TGF-β1 may be a target in chronic cholestatic liver disease.

 Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling
Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Relieves trimetazidine hypoxia / reoxygenation-induced apoptosis in neonatal rat cardiomyocytes through up-regulating the expression of HMGB1 to promote autophagy

Previous studies have shown the effects of trimetazidine (TMZ) on reducing cardiomyocyte hypoxia / reoxygenation (H / R) injury and the protective effect of autophagy on Ischemia-Reperfusion (I / R) injury to cells. However, whether TMZ protection mechanisms are also involved in autophagy remains unclear.

Our study introduces the role of HMGB1 to check TMZ on autophagy regulation on cardiomyocyte injury H / R. After cell extraction and identification by anti-α-actin staining, cardiomyocytes created hypoxia and reoxygenated, each for 3 hours, and then treated with various concentrations TMZ and transfected with siHMGB1. cell viability and apoptosis were measured by MTS method and flow cytometry, respectively. Autophagy- related factor expression (LC3-I, LC3-II, Beclin-1) and HMGB1 was detected by Western blot and qPCR. Lactate dehydrogenase (LDH) release was assessed by ELISA kit. Cardiomyocytes extracted. H / R lowering of cell viability and increase the level of LDH and cardiomyocyte apoptosis. TMZ had no effect on cardiomyocytes not treated, but reversed the adverse effects of H / R in cardiomyocytes.

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ISA34 ea
EUR 91

Silver sulfate

GK0139-100G 100 g
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Silver sulfate

GK0139-25G 25 g
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Silver sulfadiazine

GK2380-10G 10 g
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Silver sulfadiazine

GK2380-25G 25 g
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Silver stearate

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Silver stearate

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GK7224-250G 250 g
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GK7224-25G 25 g
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GX5020-25G 25 g
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GX5020-5G 5 g
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abx188878-500g 500 g
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20-abx184359
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abx097206-1Kit 1 Kit
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Sterling Rapid Silver Stain Kit

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Competent Cells, Silver Efficiency

BIO-85026 2ml ( 10 x 200µl ) Ask for price

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70034 1SET
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K2047-25 25 assays
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Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
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Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
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K2048-25 25 assays
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K2049-25 25 assays
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55R-1296 25 assays
EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

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55R-1297 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

Genorise® Alkaline Phosphatase Staining Kit

GR107045 100 tests
EUR 289

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-100
EUR 550

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-25
EUR 234

CaspGLOW? Red Active Caspase Staining Kit

K190-100
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CaspGLOW? Red Active Caspase Staining Kit

K190-25
EUR 240

Lipid (Oil Red O) staining Kit

K580-24
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Gel-FAST? Gel Staining/Destaining Kit

K901-40
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Ponceau S Staining Solution

PW005 100ml
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P7300-050 500ml
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PI / RNase Staining Buffer

20-abx090619
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EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 176

Ponceau S Staining Solution

B7778-100000 100 g
EUR 163

Ponceau S Staining Solution

B7778-50000 50 g
EUR 126

SDS-PAGE Staining Solution

GR103068 100 mL
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Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
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CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
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CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
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CASLV125PA-KIT 1 Kit
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Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
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CASLV200PA-KIT 1 Kit
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CometAssay Single Cell Gel Electrophoresis Assay-Silver

4251-050-K 1 kit
EUR 570.8

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
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Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
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Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
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Type IX Collagen Staining Kit, Multi-Species

9012 1 kit
EUR 338.55
Description: Type IX Collagen Staining Kit

Sirius Red/Fast Green Collagen Staining Kit

9046 30-50 samples
EUR 137
Description: Sirius Red/Fast Green Collagen Staining Kit

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230 50 assays
EUR 519
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230-5 5 x 50 assays
EUR 2051
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Protein Stain-EZ J Phosphoprotein Staining Kit

BSP043 2x10minigel, 20prep
EUR 110.9

Blue Feulgen DNA Ploidy Analysis Staining Kit

DPK500 1 kit(s)
EUR 356

CaspGLOW Red Active Caspase 3 Staining Kit

55R-1298 25 assays
EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 8 Staining Kit

55R-1299 25 assays
EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 9 Staining Kit

55R-1300 25 assays
EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-100
EUR 523

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-25
EUR 234

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K182-100
EUR 523

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-25
EUR 234

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-100
EUR 550

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-25
EUR 234

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-100
EUR 550

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-25
EUR 240

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-100
EUR 550

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-25
EUR 240

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-100
EUR 533

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-25
EUR 240

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-100
EUR 523

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-25
EUR 240

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-100
EUR 517

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-25
EUR 240

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

K449-50
EUR 544

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K458-50
EUR 572

EZCell? Intracellular Zinc Staining Kit (Cell-Based)

K525-50
EUR 566

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
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PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
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7-AAD Viability Staining Solution

EXB0026 400 tests
EUR 76

SafePinky DNA Gel Staining Solution

S1000-050 500ml
EUR 131

SafePinky DNA Gel Staining Solution

S1000-100 2X500ml
EUR 188

SafePinky DNA Gel Staining Solution

S1000-200 4X500ml
EUR 280

Coomassie Blue Staining Destaining Solution

AR0140 1kit (for 10 assays to stain gels of 5 x 8.5cm)
EUR 70

Coomassie Blue Fast Staining Solution

AR0170 100mL(for 10 assays to stain gels of 5 x 8.5cm)
EUR 80

7-AAD Viability Staining Buffer

abx090617-100tests 100 tests
EUR 217

Manual Staining System (12 Wells)

IMS001 1 ea.
EUR 356

Stain Tray Slide Staining System

M920-1 1 Clear
EUR 428

Stain Tray Slide Staining System

M920-2 1 Black
EUR 445

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
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AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132

Polyclonal Goat Anti-Silver homologue / Pmel 17 Antibody

AMM05119G 0.1 mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-Silver homologue / Pmel 17 . This antibody is tested and proven to work in the following applications:

Silver nitrate, 99.9%, BP, Ph. Eur., USP grade

GX5332-50G 50 g
EUR 158

Anti-Silver homologue / Pmel 17 (C-terminus) antibody

STJ70950 100 µg
EUR 359

Gelite™ Green Nucleic Acid Gel Staining Kit

17589 1 Kit
EUR 219

Gelite™ Orange Nucleic Acid Gel Staining Kit

17594 1 Kit
EUR 219

Cell Navigator™ Lysosome Staining Kit *NIR Fluorescence*

22652 500 Tests
EUR 219

Cell Navigator™ Lysosome Staining Kit *Blue Fluorescence*

22655 500 Tests
EUR 176

Cell Navigator™ Lysosome Staining Kit *Green Fluorescence*

22656 500 Tests
EUR 176

Cell Navigator™ Lysosome Staining Kit *Orange Fluorescence*

22657 500 Tests
EUR 176

Cell Navigator™ Lysosome Staining Kit *Red Fluorescence*

22658 500 Tests
EUR 176

Cell Navigator™ Mitochondrion Staining Kit *Blue Fluorescence*

22665 500 Assays
EUR 219

Cell Navigator™ Mitochondrion Staining Kit *Green Fluorescence*

22666 500 Assays
EUR 219

The expression of LC3-II, Beclin-1, and HMGB1 and the ratio of LC3-II / LC3-I increased in H / cardiomyocytes R-processed and subsequently appointed by TMZ pretreatment. However, transfection siHMGB1 exacerbate the effects of H / R in cardiomyocytes and suppresses the protective effects of TMZ on the H / R defective cardiomyocytes with increasing levels of LDH and apoptosis and reduced survival of cardiomyocytes.

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