Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Background: Following liver injury, mast cells (MC) migrate to the liver and is activated in patients with cholestasis. Inhibition of MC decreased mediator ductular reaction (DR) and liver fibrosis. TGF-β1 contributes to fibrosis and promote liver disease.


Objective: To demonstrate that MC induces reintroduction of cholestatic injury with TGF-β1.


Methods: Wild-type, KitW-sh (MC-deficient), and Mdr2 – / – mice lacking L-histidine decarboxylase injected with vehicle or PKH26-tagged murine MC by treatment with 0.01% DMSO or inhibitor of TGF-βR, LY2109761 (TGF-βRi, 10 M) three days before the bag. liver damage was assessed by H & E and serum chemistry. MC injected detected in the liver, spleen and lungs by immunofluorescence (IF). DR is measured by CK-19 immunohistochemistry (IHC) and F4 / 80 staining coupled with qPCR for IL-1β, IL-33 and F4 / 80; aging bile evaluated by IF or qPCR for p16, p18 and p21. Fibrosis was evaluated by Sirius Red / Fast Green staining and IF to SYP 9, desmin, and α-SMA. secretion of TGF-β1 / expression was measured by EIA and qPCR. Angiogenesis is detected by the IF to vonWillebrand Factor and VEGF-C qPCR. In vitro, MC TGF-β1 expression / secretion was measured after treatment of TGF-βRi; conditioned media is collected. Cholangiocytes and hepatic stellate cell (HSCs) were treated with conditioned medium and bile MC proliferation / aging is measured by MTS and qPCR; HSC activation was evaluated for α-SMA, SYP-9 and expression of collagen type-1a.


Results: MC injection recapitulation cholestatic liver injury is characterized by an increase in DR; fibrosis / TGF-β1 secretion; and angiogenesis. Injection MC-TGF-βRi reverse this parameter. In vitro, induces gall MC proliferation / activation of HSC aging and overturned by MC less TGF-β1.


Conclusion: Our new research shows that the reintroduction of cholestatic liver injury mimics MC and MC derived TGF-β1 may be a target in chronic cholestatic liver disease.

 Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling
Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Relieves trimetazidine hypoxia / reoxygenation-induced apoptosis in neonatal rat cardiomyocytes through up-regulating the expression of HMGB1 to promote autophagy

Previous studies have shown the effects of trimetazidine (TMZ) on reducing cardiomyocyte hypoxia / reoxygenation (H / R) injury and the protective effect of autophagy on Ischemia-Reperfusion (I / R) injury to cells. However, whether TMZ protection mechanisms are also involved in autophagy remains unclear.

Our study introduces the role of HMGB1 to check TMZ on autophagy regulation on cardiomyocyte injury H / R. After cell extraction and identification by anti-α-actin staining, cardiomyocytes created hypoxia and reoxygenated, each for 3 hours, and then treated with various concentrations TMZ and transfected with siHMGB1. cell viability and apoptosis were measured by MTS method and flow cytometry, respectively. Autophagy- related factor expression (LC3-I, LC3-II, Beclin-1) and HMGB1 was detected by Western blot and qPCR. Lactate dehydrogenase (LDH) release was assessed by ELISA kit. Cardiomyocytes extracted. H / R lowering of cell viability and increase the level of LDH and cardiomyocyte apoptosis. TMZ had no effect on cardiomyocytes not treated, but reversed the adverse effects of H / R in cardiomyocytes.

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 276

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

Sterling Rapid Silver Stain Kit

NAT1106 1KIT
EUR 300

ELVIS ID Staining Kit

SK-ELVIS-100 100 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-1000 1000 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-200 200 Tests Ask for price

ELVIS ID Staining Kit

SK-ELVIS-500 500 Tests Ask for price

?-Galactosidase Staining Kit

AKR-100 1 kit
EUR 518.4
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 276

Cell Cycle Staining Kit

KTA2020-100T 100 T
EUR 119
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-50T 50 T
EUR 69
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Live NucRed staining kit

EGY069 >100T
EUR 210

Live NucGreen staining kit

EGY068 >100T
EUR 210

Dead NucGreen staining kit

EGY070 >100T
EUR 210

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 494.4

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 314.4

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 765.6
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 444
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 203

Human Histochemistry Staining Kit

20-abx299039
  • EUR 1045.20
  • EUR 1462.80
  • EUR 678.00
  • 150 tests
  • 500 tests
  • 50 tests

Beta-Galactosidase Staining Kit

K802-250 each
EUR 352.8

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 351.6

Live-Dead Cell Staining Kit

K501-100 each
EUR 561.6

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 552

Live-Dead Cell Staining Kit

K2081-1000 1000T
EUR 140

Live-Dead Cell Staining Kit

K2081-500 500T
EUR 85

Purple-Color AP Staining Kit

AP100P-1 100 Assays
EUR 235

Nerve Terminal Staining Kit V

70034 1SET
EUR 610.8
Description: Minimum order quantity: 1 unit of 1SET

STAT Double Staining ragent for Double staining Mo

NB330DB 25 ml
EUR 595
Description: STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 686.4

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 285.6

STAT Double Staining reagent for Double staining o

NB326DB 25 ml
EUR 595
Description: STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides

STAT Double Staining Reagent for Double staining o

NB327DB 25 ml
EUR 595
Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

Lipid (Oil Red O) staining Kit

K580-24 each
EUR 542.4

DNA Ploidy Analysis Staining Kit

K1439-1 each
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5 each
EUR 2402.4

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 249.6

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 153.6

DAPI staining kit (ready to use) 

EGY0621 10mL
EUR 85

DAPI staining kit (ready to use) 

EGY0622 50mL
EUR 185

Stayrightâ„¢ Purple HRP Staining Kit

45905-5mL 5 mL
EUR 109
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45906-50mL 50 mL
EUR 334
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Hematoxylin and Eosin Staining Kit

K1142-1000 2×500mL
EUR 78
Description: Hematoxylin and Eosin Staining,one of the most widely used staining technique in histopathology

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 355.2
Description: Caspase Staining Kit for use activity in the research laboratory

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 292.8

Human Type I Collagen Staining Kit

9044 1 kit
EUR 406.26
Description: Human Type I Collagen Staining Kit

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 686.4

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 285.6

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 686.4

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 285.6

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 686.4

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 285.6

Calcein-AM/PI Double Staining Kit

E-CK-A354-100Assays 100 Assays
EUR 100

Calcein-AM/PI Double Staining Kit

E-CK-A354-2000Assays 2000 Assays
EUR 600

Calcein-AM/PI Double Staining Kit

E-CK-A354-500Assays 500 Assays
EUR 300

Stayright™ Purple HRP Staining Kit

45905 5 mL
EUR 109

Stayright™ Purple HRP Staining Kit

45906 50 mL
EUR 334

Fluorescein Active Caspase Staining Kit

K2047-100 100 assays
EUR 686.4

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 285.6

Foxp3/Transcription Factor Staining Kit

E-CK-A108-20Assays 20 Assays
EUR 75

Four color suit nuclear staining Kit

EGY071 100T
EUR 1190

Hoechst 33342 staining kit (ready to use) 

EGY0631 10mL
EUR 50

Hoechst 33342 staining kit (ready to use) 

EGY0632 50mL
EUR 160

Hoechst 33258 staining kit (ready to use) 

EGY0641 10mL
EUR 50

Hoechst 33258 staining kit (ready to use) 

EGY0642 50mL
EUR 145

Senescence β-Galactosidase Staining Kit

KTA3030-100T 100 T
EUR 119
Description: Senescence β-galactosidase staining kit provides a simple and convenient operation method for staining detection of senescent cells or tissues; This kit only stains senescent cells, and does not stain pre-senescence cells (senescent cells), quiescent cells (static cells), immortal cells (immortal cells) or tumor cells.

Yeast Viability Staining Kit (Green/Red)

31063-1 1kit
EUR 566.4
Description: Minimum order quantity: 1 unit of 1kit

Coplin Staining Jar

08415-3 3sets
EUR 84

CaspGLOW Active Caspase 12 Staining Kit

55R-1291 25 assays
EUR 338.4
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit

55R-1293 25 assays
EUR 340.8
Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit

55R-1294 25 assays
EUR 355.2
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit

55R-1295 25 assays
EUR 355.2
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 9 Staining Kit

55R-1296 25 assays
EUR 355.2
Description: Caspase 9 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase Staining Kit

55R-1297 25 assays
EUR 355.2
Description: Caspase Staining Kit for use activity in the research laboratory

CaspGLOW? Red Active Caspase Staining Kit

K190-100 each
EUR 627.6

CaspGLOW? Red Active Caspase Staining Kit

K190-25 each
EUR 288

Nuclear 7-AAD fluorescent staining kit

EGY066 >100T
EUR 195

Cell Staining Buffer

E16FXP005 500 ml
EUR 40
Description: Available in various conjugation types.

Cell Staining Buffer

E-CK-A107-100mL 100 mL
EUR 20

Cell Staining Buffer

E-CK-A107-200mL 200 mL
EUR 35

Cell Staining Buffer

E-CK-A107-500mL 500 mL
EUR 60

Live and Dead Cell Double Staining Kit

KTA1001-100T 100 T
EUR 79
Description: Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells.

Live and Dead Cell Double Staining Kit

KTA1001-2000T 2000 T
EUR 239
Description: Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells.

Live and Dead Cell Double Staining Kit

KTA1001-500T 500 T
EUR 139
Description: Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells.

Oxidizer Solution (For Steiner's Silver Stain)

SOS125 125 ml
EUR 91.2

Oxidizer Solution (For Steiner's Silver Stain)

SOS500 500 ml
EUR 133.2

Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
EUR 226.8

Fluorescein Active Caspase-2 Staining Kit

K2048-100 100 assays
EUR 686.4

Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
EUR 285.6

Fluorescein Active Caspase-3 Staining Kit

K2049-100 100 assays
EUR 686.4

Fluorescein Active Caspase-3 Staining Kit

K2049-25 25 assays
EUR 285.6

Fluorescein Active Caspase-8 Staining Kit

K2050-100 100 assays
EUR 686.4

Fluorescein Active Caspase-8 Staining Kit

K2050-25 25 assays
EUR 285.6

Fluorescein Active Caspase-9 Staining Kit

K2051-100 100 assays
EUR 686.4

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 285.6

Fluorescein Active Caspase-12 Staining Kit

K2046-100 100 assays
EUR 686.4

Fluorescein Active Caspase-12 Staining Kit

K2046-25 25 assays
EUR 285.6

Protein Stain-EZ J Phosphoprotein Staining Kit

BSP043 2x10minigel, 20prep
EUR 133.08

Propidium iodide staining kit (ready to use) 

EGY0651 5mL
EUR 90

Propidium iodide staining kit (ready to use) 

EGY0652 10mL
EUR 125

Propidium iodide staining kit (ready to use) 

EGY0653 20mL
EUR 240

StemTAG Alkaline Phosphatase Staining Kit (Red)

CBA-300 100 assays
EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Human FoxP3 Transcription Factor Staining Kit

OKTA00001 KIT
EUR 544.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Human FoxP3 Transcription Factor Staining Kit

OKTA00004 KIT
EUR 544.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Genorise® Alkaline Phosphatase Staining Kit

GR107045 100 tests
EUR 289

CaspGLOW Red Active Caspase 3 Staining Kit

55R-1298 25 assays
EUR 355.2
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 8 Staining Kit

55R-1299 25 assays
EUR 355.2
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 9 Staining Kit

55R-1300 25 assays
EUR 355.2
Description: Caspase 9 Staining Kit for use activity in the research laboratory

Geliteâ„¢ Green Nucleic Acid Gel Staining Kit

17589-1Kit 1 Kit
EUR 222
Description: Gelite™ Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels.

Sirius Red/Fast Green Collagen Staining Kit

9046 30-50 samples
EUR 164.4
Description: Sirius Red/Fast Green Collagen Staining Kit

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-100 each
EUR 639.6

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-25 each
EUR 288

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-100 each
EUR 620.4

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-25 each
EUR 288

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-100 each
EUR 627.6

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-25 each
EUR 288

Geliteâ„¢ Orange Nucleic Acid Gel Staining Kit

17594-1Kit 1 Kit
EUR 222
Description: Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film.

Type IX Collagen Staining Kit, Multi-Species

9012 1 kit
EUR 406.26
Description: Type IX Collagen Staining Kit

Blue Feulgen DNA Ploidy Analysis Staining Kit

DPK500 1 kit(s)
EUR 427.2

StemTAG Alkaline Phosphatase Staining Kit (Purple)

CBA-306 100 assays
EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-25 each
EUR 280.8

PI / RNase Staining Buffer

20-abx090619
  • EUR 326.40
  • EUR 276.00
  • 100 tests
  • 50 tests

Exosome fluorescent staining

S104 -
EUR 800

HISTOCHEMISTRY STAINING TRAY

AT-12 12 PLACE - BLACK LID
EUR 226.1

HISTOCHEMISTRY STAINING TRAY

AT-24 24 PLACE - BLACK LID
EUR 342.21

HISTOCHEMISTRY STAINING TRAY

ATC-12 12 PLACE - CLEAR LID
EUR 226.1

HISTOCHEMISTRY STAINING TRAY

ATC-24 24 PLACE - CLEAR LID
EUR 342.21

Gel-FAST? Gel Staining/Destaining Kit

K901-40 each
EUR 210

EnoGeneEXBlueTM One Step Rapid Gel Staining kit

EGU002-100 100ml
EUR 60

EnoGeneEXBlueTM One Step Rapid Gel Staining kit

EGU002-500 500ml
EUR 90

Gelite™ Green Nucleic Acid Gel Staining Kit

17589 1 Kit
EUR 222

Live-or-Dye NuCFix Red Staining Kit (200 assays)

32010 1kit
EUR 326.4
Description: Minimum order quantity: 1 unit of 1kit

Phosphorylated protein gel fluorescent staining kit

E1WP329 250mL / 5 gels
EUR 300

Gelite™ Orange Nucleic Acid Gel Staining Kit

17594 1 Kit
EUR 222

TraKine™ Mitochondrion and Nuclear Staining Kit

KTC4005-100T 100 T
EUR 79
Description: Abbkine TraKine™ Mitochondrion and Nuclear Staining Kit provides two stain for mitochondrial and nuclear staining.

TraKine™ Mitochondrion and Nuclear Staining Kit

KTC4005-2000T 2000 T
EUR 559
Description: Abbkine TraKine™ Mitochondrion and Nuclear Staining Kit provides two stain for mitochondrial and nuclear staining.

TraKine™ Mitochondrion and Nuclear Staining Kit

KTC4005-500T 500 T
EUR 229
Description: Abbkine TraKine™ Mitochondrion and Nuclear Staining Kit provides two stain for mitochondrial and nuclear staining.

GEL STAINING/INCUBATION BOX

SB-10 LIGHT SHIELDING
EUR 94.44

Cell Navigator® Live Cell Tubulin Staining Kit

23170 100 Slides
EUR 222

Cell Navigator® Live Cell Tubulin Staining Kit

23171 300 Slides
EUR 446

Ponceau S Staining Solution

B7778-100000 100 g
EUR 195.6

Ponceau S Staining Solution

B7778-50000 50 g
EUR 151.2

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 58

Ponceau S Staining Solution

PW005 100ml
EUR 82.97

Ponceau S Staining Solution

41640009-1 500 mL
EUR 40.13

Ponceau S Staining Solution

41640009-2 1 L
EUR 58.16

EZCell? Intracellular Zinc Staining Kit (Cell-Based)

K525-50 each
EUR 679.2

EnoGeneEXBlueTM One Step Rapid Protein Staining Kit

EGQ0051 100ml
EUR 145

EnoGeneEXBlueTM One Step Rapid Protein Staining Kit

EGQ0052 500ml
EUR 225

Cell Navigator® Live Cell Tubulin Staining Kit

23170-100Slides 100 Slides
EUR 222
Description: Cell Navigator® Live Cell Tubulin Staining Kit provides a robust method to fluorescently image tubulins in live cells with Tubulite™ Red.

Cell Navigator® Live Cell Tubulin Staining Kit

23171-300Slides 300 Slides
EUR 446
Description: Cell Navigator® Live Cell Tubulin Staining Kit provides a robust method to fluorescently image tubulins in live cells with Tubulite™ Red.

Oil Red O Lipid Staining Kit, Catalog: COS-0101

COS-0101 To stain two plates (6,12, 24 or 96well) or five 100 mm culture dishes
EUR 370

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-25 each
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-100 each
EUR 627.6

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-25 each
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-25 each
EUR 288

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-25 each
EUR 288

Beta-galactosidase Staining Kit, Catalog: COS-0104

COS-0104 To stain 20 X 12well plates or the equivalent volume of 6 or 24well plates.
EUR 265

Alkaline Phosphatase Staining Kit, Catalog: COS-0105

COS-0105 To stain 96, 48, 24, 12, 6well plates
EUR 325

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 211.2

PAP PEN FOR IMMUNO STAINING

Z377821-1EA EACH
EUR 171.03

Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based)

K458-50 each
EUR 686.4

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

K449-50 each
EUR 652.8

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-100 each
EUR 627.6

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-25 each
EUR 280.8

Nalgene Box Staining 13x13x5cm

BOX1040 EACH
EUR 48

PI Viability Staining Solution

E16FXP002 150 tests
EUR 40
Description: Available in various conjugation types.

TraKine™ F-actin Staining Kit (Green Fluorescence)

KTC4008-1000T 1000 T
EUR 559
Description: Abbkine TraKine™ F-actin Staining Kit (Green Fluorescence) is designed to label F-actins in fixed cells with green fluorescence.

TraKine™ F-actin Staining Kit (Green Fluorescence)

KTC4008-300T 300 T
EUR 199
Description: Abbkine TraKine™ F-actin Staining Kit (Green Fluorescence) is designed to label F-actins in fixed cells with green fluorescence.

TraKine™ F-actin Staining Kit (Green Fluorescence)

KTC4008-50T 50 T
EUR 59
Description: Abbkine TraKine™ F-actin Staining Kit (Green Fluorescence) is designed to label F-actins in fixed cells with green fluorescence.

OxiSelect DNA Double-Strand Break (DSB) Staining Kit

STA-321 100 assays
EUR 790.8
Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.

Disposable Slide Staining Trays

MIC5070 PK4
EUR 90

Staining Trough+Lid Rectangular

MIC6008 EACH
EUR 27.6

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

Wheaton staining dishes 3 EA

S5017-3EA PK3
EUR 135.6

EZ-Quick Slide Staining Set

IW-2510 -
EUR 425

CellBrite™ Steady 405 Membrane Staining Kit, 500 Labelings

30105 -
EUR 868
Description: N/A

CellBrite™ Steady 405 Membrane Staining Kit, 100 Labelings

30105-T -
EUR 232
Description: N/A

CellBrite™ Steady 488 Membrane Staining Kit, 500 Labelings

30106 -
EUR 868
Description: N/A

CellBrite™ Steady 488 Membrane Staining Kit, 100 Labelings

30106-T -
EUR 232
Description: N/A

CellBrite™ Steady 550 Membrane Staining Kit, 500 Labelings

30107 -
EUR 868
Description: N/A

CellBrite™ Steady 550 Membrane Staining Kit, 100 Labelings

30107-T -
EUR 232
Description: N/A

CellBrite™ Steady 650 Membrane Staining Kit, 500 Labelings

30108 -
EUR 868
Description: N/A

CellBrite™ Steady 650 Membrane Staining Kit, 100 Labelings

30108-T -
EUR 232
Description: N/A

CellBrite™ Steady 685 Membrane Staining Kit, 500 Labelings

30109 -
EUR 868
Description: N/A

The expression of LC3-II, Beclin-1, and HMGB1 and the ratio of LC3-II / LC3-I increased in H / cardiomyocytes R-processed and subsequently appointed by TMZ pretreatment. However, transfection siHMGB1 exacerbate the effects of H / R in cardiomyocytes and suppresses the protective effects of TMZ on the H / R defective cardiomyocytes with increasing levels of LDH and apoptosis and reduced survival of cardiomyocytes.

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