Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Background: Following liver injury, mast cells (MC) migrate to the liver and is activated in patients with cholestasis. Inhibition of MC decreased mediator ductular reaction (DR) and liver fibrosis. TGF-β1 contributes to fibrosis and promote liver disease.


Objective: To demonstrate that MC induces reintroduction of cholestatic injury with TGF-β1.


Methods: Wild-type, KitW-sh (MC-deficient), and Mdr2 – / – mice lacking L-histidine decarboxylase injected with vehicle or PKH26-tagged murine MC by treatment with 0.01% DMSO or inhibitor of TGF-βR, LY2109761 (TGF-βRi, 10 M) three days before the bag. liver damage was assessed by H & E and serum chemistry. MC injected detected in the liver, spleen and lungs by immunofluorescence (IF). DR is measured by CK-19 immunohistochemistry (IHC) and F4 / 80 staining coupled with qPCR for IL-1β, IL-33 and F4 / 80; aging bile evaluated by IF or qPCR for p16, p18 and p21. Fibrosis was evaluated by Sirius Red / Fast Green staining and IF to SYP 9, desmin, and α-SMA. secretion of TGF-β1 / expression was measured by EIA and qPCR. Angiogenesis is detected by the IF to vonWillebrand Factor and VEGF-C qPCR. In vitro, MC TGF-β1 expression / secretion was measured after treatment of TGF-βRi; conditioned media is collected. Cholangiocytes and hepatic stellate cell (HSCs) were treated with conditioned medium and bile MC proliferation / aging is measured by MTS and qPCR; HSC activation was evaluated for α-SMA, SYP-9 and expression of collagen type-1a.


Results: MC injection recapitulation cholestatic liver injury is characterized by an increase in DR; fibrosis / TGF-β1 secretion; and angiogenesis. Injection MC-TGF-βRi reverse this parameter. In vitro, induces gall MC proliferation / activation of HSC aging and overturned by MC less TGF-β1.


Conclusion: Our new research shows that the reintroduction of cholestatic liver injury mimics MC and MC derived TGF-β1 may be a target in chronic cholestatic liver disease.

 Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling
Mast cells (MCs) induce ductular reaction mimicking liver injury in mice via MC-derived TGF-β1 signaling

Relieves trimetazidine hypoxia / reoxygenation-induced apoptosis in neonatal rat cardiomyocytes through up-regulating the expression of HMGB1 to promote autophagy

Previous studies have shown the effects of trimetazidine (TMZ) on reducing cardiomyocyte hypoxia / reoxygenation (H / R) injury and the protective effect of autophagy on Ischemia-Reperfusion (I / R) injury to cells. However, whether TMZ protection mechanisms are also involved in autophagy remains unclear.

Our study introduces the role of HMGB1 to check TMZ on autophagy regulation on cardiomyocyte injury H / R. After cell extraction and identification by anti-α-actin staining, cardiomyocytes created hypoxia and reoxygenated, each for 3 hours, and then treated with various concentrations TMZ and transfected with siHMGB1. cell viability and apoptosis were measured by MTS method and flow cytometry, respectively. Autophagy- related factor expression (LC3-I, LC3-II, Beclin-1) and HMGB1 was detected by Western blot and qPCR. Lactate dehydrogenase (LDH) release was assessed by ELISA kit. Cardiomyocytes extracted. H / R lowering of cell viability and increase the level of LDH and cardiomyocyte apoptosis. TMZ had no effect on cardiomyocytes not treated, but reversed the adverse effects of H / R in cardiomyocytes.

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

Sterling Rapid Silver Stain Kit

NAT1106 1KIT
EUR 300

?-Galactosidase Staining Kit

AKR-100 1 kit
EUR 518.4
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 276

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 444
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 494.4

Human Histochemistry Staining Kit

20-abx299039
  • EUR 1045.20
  • EUR 1462.80
  • EUR 678.00
  • 150 tests
  • 500 tests
  • 50 tests

Beta-Galactosidase Staining Kit

K802-250 each
EUR 352.8

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 351.6

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 765.6
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 314.4

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 203

Live-Dead Cell Staining Kit

K501-100 each
EUR 561.6

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 552

Nerve Terminal Staining Kit V

70034 1SET
EUR 610.8
Description: Minimum order quantity: 1 unit of 1SET

Purple-Color AP Staining Kit

AP100P-1 100 Assays
EUR 235

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 686.4

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 285.6

STAT Double Staining ragent for Double staining Mo

NB330DB 25 ml
EUR 595
Description: STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides

STAT Double Staining reagent for Double staining o

NB326DB 25 ml
EUR 595
Description: STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides

STAT Double Staining Reagent for Double staining o

NB327DB 25 ml
EUR 595
Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

DNA Ploidy Analysis Staining Kit

K1439-1 each
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5 each
EUR 2402.4

Lipid (Oil Red O) staining Kit

K580-24 each
EUR 542.4

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 249.6

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 153.6

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 355.2
Description: Caspase Staining Kit for use activity in the research laboratory

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 292.8

Fluorescein Active Caspase Staining Kit

K2047-100 100 assays
EUR 686.4

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 285.6

Human Type I Collagen Staining Kit

9044 1 kit
EUR 406.26
Description: Human Type I Collagen Staining Kit

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 686.4

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 285.6

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 686.4

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 285.6

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 686.4

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 285.6

Stayright™ Purple HRP Staining Kit

45905 5 mL
EUR 109

Stayright™ Purple HRP Staining Kit

45906 50 mL
EUR 334

Yeast Viability Staining Kit (Green/Red)

31063-1 1kit
EUR 566.4
Description: Minimum order quantity: 1 unit of 1kit

CaspGLOW Active Caspase 12 Staining Kit

55R-1291 25 assays
EUR 338.4
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit

55R-1293 25 assays
EUR 340.8
Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit

55R-1294 25 assays
EUR 355.2
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit

55R-1295 25 assays
EUR 355.2
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 9 Staining Kit

55R-1296 25 assays
EUR 355.2
Description: Caspase 9 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase Staining Kit

55R-1297 25 assays
EUR 355.2
Description: Caspase Staining Kit for use activity in the research laboratory

CaspGLOW? Red Active Caspase Staining Kit

K190-100 each
EUR 627.6

CaspGLOW? Red Active Caspase Staining Kit

K190-25 each
EUR 288

Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
EUR 226.8

Fluorescein Active Caspase-2 Staining Kit

K2048-100 100 assays
EUR 686.4

Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
EUR 285.6

Fluorescein Active Caspase-3 Staining Kit

K2049-100 100 assays
EUR 686.4

Fluorescein Active Caspase-3 Staining Kit

K2049-25 25 assays
EUR 285.6

Fluorescein Active Caspase-8 Staining Kit

K2050-100 100 assays
EUR 686.4

Fluorescein Active Caspase-8 Staining Kit

K2050-25 25 assays
EUR 285.6

Fluorescein Active Caspase-9 Staining Kit

K2051-100 100 assays
EUR 686.4

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 285.6

Fluorescein Active Caspase-12 Staining Kit

K2046-100 100 assays
EUR 686.4

Fluorescein Active Caspase-12 Staining Kit

K2046-25 25 assays
EUR 285.6

StemTAG Alkaline Phosphatase Staining Kit (Red)

CBA-300 100 assays
EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Human FoxP3 Transcription Factor Staining Kit

OKTA00001 KIT
EUR 544.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Human FoxP3 Transcription Factor Staining Kit

OKTA00004 KIT
EUR 544.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Genorise® Alkaline Phosphatase Staining Kit

GR107045 100 tests
EUR 289

Oxidizer Solution (For Steiner's Silver Stain)

SOS125 125 ml
EUR 91.2

Oxidizer Solution (For Steiner's Silver Stain)

SOS500 500 ml
EUR 133.2

Protein Stain-EZ J Phosphoprotein Staining Kit

BSP043 2x10minigel, 20prep
EUR 133.08

CaspGLOW Red Active Caspase 3 Staining Kit

55R-1298 25 assays
EUR 355.2
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 8 Staining Kit

55R-1299 25 assays
EUR 355.2
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 9 Staining Kit

55R-1300 25 assays
EUR 355.2
Description: Caspase 9 Staining Kit for use activity in the research laboratory

Sirius Red/Fast Green Collagen Staining Kit

9046 30-50 samples
EUR 164.4
Description: Sirius Red/Fast Green Collagen Staining Kit

StemTAG Alkaline Phosphatase Staining Kit (Purple)

CBA-306 100 assays
EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-25 each
EUR 280.8

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-100 each
EUR 627.6

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-25 each
EUR 288

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-100 each
EUR 620.4

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-25 each
EUR 288

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-100 each
EUR 639.6

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-25 each
EUR 288

Type IX Collagen Staining Kit, Multi-Species

9012 1 kit
EUR 406.26
Description: Type IX Collagen Staining Kit

Blue Feulgen DNA Ploidy Analysis Staining Kit

DPK500 1 kit(s)
EUR 427.2

Gel-FAST? Gel Staining/Destaining Kit

K901-40 each
EUR 210

PI / RNase Staining Buffer

20-abx090619
  • EUR 326.40
  • EUR 276.00
  • 100 tests
  • 50 tests

Gelite™ Green Nucleic Acid Gel Staining Kit

17589 1 Kit
EUR 222

Gelite™ Orange Nucleic Acid Gel Staining Kit

17594 1 Kit
EUR 222

Ponceau S Staining Solution

B7778-100000 100 g
EUR 195.6

Ponceau S Staining Solution

B7778-50000 50 g
EUR 151.2

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 58

Ponceau S Staining Solution

PW005 100ml
EUR 82.97

Live-or-Dye NuCFix Red Staining Kit (200 assays)

32010 1kit
EUR 326.4
Description: Minimum order quantity: 1 unit of 1kit

EZCell? Intracellular Zinc Staining Kit (Cell-Based)

K525-50 each
EUR 679.2

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 211.2

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-100 each
EUR 627.6

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-25 each
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-25 each
EUR 288

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-25 each
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-25 each
EUR 288

Cell Navigator® Live Cell Tubulin Staining Kit

23170 100 Slides
EUR 222

Cell Navigator® Live Cell Tubulin Staining Kit

23171 300 Slides
EUR 446

Nalgene Box Staining 13x13x5cm

BOX1040 EACH
EUR 48

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

K449-50 each
EUR 652.8

Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based)

K458-50 each
EUR 686.4

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-100 each
EUR 627.6

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-25 each
EUR 280.8

Disposable Slide Staining Trays

MIC5070 PK4
EUR 90

Staining Trough+Lid Rectangular

MIC6008 EACH
EUR 27.6

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

PAP PEN FOR IMMUNO STAINING

Z377821-1EA EACH
EUR 171.03

Stain Tray Slide Staining System

M920-1 1 Clear
EUR 513.6

Stain Tray Slide Staining System

M920-2 1 Black
EUR 534

OxiSelect DNA Double-Strand Break (DSB) Staining Kit

STA-321 100 assays
EUR 790.8
Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.

Wheaton staining dishes 3 EA

S5017-3EA PK3
EUR 135.6

Manual Staining System (12 Wells)

IMS001 1 ea.
EUR 427.2

Staining Jar W Lid 115x115x77mm

MIC6016 PK10
EUR 428.4

CaspGLOW™ Green Active Caspase-3/7 Staining Kit

K2030-25 25 assays
EUR 272.4

Staining Jar PP + Lid 10 Slide

MIC6002 EACH
EUR 67.2

Cell Navigator® Lysosome Staining Kit *NIR Fluorescence*

22652 500 Tests
EUR 222

Cell Navigator® Lysosome Staining Kit *Red Fluorescence*

22658 500 Tests
EUR 166

Staining Dish with Cover Plastic

X24085 EACH
EUR 156

Cell Navigator® Lysosome Staining Kit *Blue Fluorescence*

22655 500 Tests
EUR 166

7-AAD Viability Staining Buffer

abx090617-100tests 100 tests
EUR 260.4

Simport EasyDip Staining Jar Blue

HIS0273 PK6
EUR 117.6

Simport EasyDip Staining Jar Pink

HIS0275 PK6
EUR 117.6

Cell Navigator® Lysosome Staining Kit *Green Fluorescence*

22656 500 Tests
EUR 166

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230 50 assays
EUR 622.8
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230-5 5 x 50 assays
EUR 2461.2
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Simport EasyDip Staining Jar Green

HIS0274 PK6
EUR 117.6

Simport EasyDip Staining Jar White

HIS0276 PK6
EUR 110.4

Simport EasyDip Staining Rack Grey

HIS0278 PK6
EUR 128.4

Cell Navigator® Lysosome Staining Kit *Orange Fluorescence*

22657 500 Tests
EUR 166

7-AAD Viability Staining Solution

EXB0026 400 tests
EUR 91.2

Simport EasyDip Staining Jar Yellow

HIS0277 PK6
EUR 110.4

SafePinky DNA Gel Staining Solution

S1000-050 500ml
EUR 157.2

SafePinky DNA Gel Staining Solution

S1000-100 2X500ml
EUR 225.6

SafePinky DNA Gel Staining Solution

S1000-200 4X500ml
EUR 336

Cell Navigator® Mitochondrion Staining Kit *Red Fluorescence*

22668 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *NIR Fluorescence*

22670 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *Blue Fluorescence*

22665 500 Assays
EUR 222

Coomassie Blue Fast Staining Solution

AR0170 100mL(for 10 assays to stain gels of 5 x 8.5cm)
EUR 96

Staining Jar Schiefferdecker With Lid

MIC6014 EACH
EUR 22.8

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD)

STA-327 96 assays
EUR 790.8
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

Cell Navigator® Mitochondrion Staining Kit *Green Fluorescence*

22666 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *Orange Fluorescence*

22667 500 Assays
EUR 222

Mouse to Mouse HRP Staining System

MTM003 400 Slides
EUR 433.2

Cell Navigator® Lysosome Staining Kit *Deep Red Fluorescence*

22659 500 Tests
EUR 166

Live-or-Dye UV/448 Fixable Viability Staining Kit (200 assays)

32002 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 405/452 Fixable Viability Staining Kit (200 assays)

32003 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 488/515 Fixable Viability Staining Kit (200 assays)

32004 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 568/583 Fixable Viability Staining Kit (200 assays)

32005 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 594/614 Fixable Viability Staining Kit (200 assays)

32006 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 640/662 Fixable Viability Staining Kit (200 assays)

32007 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 750/777 Fixable Viability Staining Kit (200 assays)

32008 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 405/545 Fixable Viability Staining Kit (200 assays)

32009 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Hellandahl Staining Jar+Lid 16 slide

MIC6004 EACH
EUR 34.8

MemBriteâ„¢ Fix-ST 650/665 Cell Surface Staining Kit, 500 Reactions

30101 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 650/665 Cell Surface Staining Kit, 100 Reactions

30101-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 667/685 Cell Surface Staining Kit, 500 Reactions

30102 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 667/685 Cell Surface Staining Kit, 100 Reactions

30102-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 681/698 Cell Surface Staining Kit, 500 Reactions

30103 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 681/698 Cell Surface Staining Kit, 100 Reactions

30103-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 755/777 Cell Surface Staining Kit, 500 Reactions

30104 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 755/777 Cell Surface Staining Kit, 100 Reactions

30104-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

Coplin Staining Jar Glass+Lid 10Slide

MIC6000 EACH
EUR 13.2

STAT-Q Multivalent AEC KIT for staining Mouse, Rat

NB314KLC 1000 slide kit
EUR 875
Description: STAT-Q Multivalent AEC KIT for staining Mouse, Rat and Rabbit antibodies, 1000 plus slides (Large size kit)

STAT-Q Multivalent AEC KIT for staining Mouse, Rat

NB314KLC-20 300 slides
EUR 595
Description: STAT-Q Multivalent AEC KIT for staining Mouse, Rat and Rabbit antibodies, 350 plus slides (Small size kit)

STAT-Q Multivalent DAB KIT for staining Mouse, Rat

NB314KLD 1000 slides
EUR 895
Description: STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 1000 plus slides (Large size kit)

STAT-Q Multivalent DAB KIT for staining Mouse, Rat

NB314KLD-20 300 slide kit
EUR 595
Description: STAT-Q Multivalent DAB KIT for staining Mouse, Rat and Rabbit antibodies, 350 slides kit (Small size Kit)

OxiSelect DNA Double Strand Break (DSB) Staining Kit, Trial Size

STA-321-T 20 assays
EUR 421.2
Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.

Vertical Slide Staining Rack Dark Gray

M905-12DGY 1 Case(s)
EUR 220.8

Live-or-Dye NuCFix Red Staining Kit, Trial Size (50 assays)

32010-T 1kit
EUR 139.2
Description: Minimum order quantity: 1 unit of 1kit

BRAND(TM) STAINING TRAY FOR 25 SLIDES

BR471400-5EA PK5
EUR 58.1

BRAND(TM) STAINING TRAY FOR 20 SLIDES

BR474305-2EA PK2
EUR 43.2

Coomassie Blue Staining Destaining Solution

AR0140 1kit (for 10 assays to stain gels of 5 x 8.5cm)
EUR 84

STAT-Q Goat AEC KIT for staining all Goat primary

NB314KLGC-20 20 ml kit
EUR 589
Description: STAT-Q Goat AEC KIT for staining all Goat primary antibodies, 350 plus slides

STAT-Q Goat DAB KIT for staining all Goat primary

NB314KLGD-20 20 ml kit
EUR 589
Description: STAT-Q Goat DAB KIT for staining all Goat primary antibodies, 350 plus slides

STAT-Q Mouse Adsorbed AEC KIT for staining Rat an

NB314MAK-AEC 200-250 slide kit
EUR 695
Description: STAT-Q Mouse Adsorbed AEC KIT for staining Rat antibodies-on-Mouse tissues 250 plus slide kit

Cell Navigator® Mitochondrion Staining Kit *Deep Red Fluorescence*

22669 500 Assays
EUR 222

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP)

STA-329 96 assays
EUR 790.8
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of 6-4PP structures in DNA by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

Coomassie Blue G-250 Staining Solution

GR103035 1 L
EUR 95

STAT-Q Rabbit AEC KIT for staining all Rabbit anti

NB314KLRC-20 20 ml kit
EUR 589
Description: STAT-Q Rabbit AEC KIT for staining all Rabbit antibodies, 350 plus slides

STAT-Q Rabbit DAB KIT for staining all Rabbit anti

NB314KLRD-20 20 ml kit
EUR 585
Description: STAT-Q Rabbit DAB KIT for staining all Rabbit antibodies, 350 plus slides

STAT-Q Mouse Adsorbed DAB KIT for staining Rat ant

NB314MAK-DAB 200-250 slides
EUR 695
Description: STAT-Q Mouse Adsorbed DAB KIT for staining Rat antibodies-on-Mouse tissues 250 plus slide kit

STAT-Q Rat Adsorbed AEC KIT for staining Mouse ant

NB314RAK-AEC 200-250 slide kit
EUR 695
Description: STAT-Q Rat Adsorbed AEC KIT for staining Mouse antibodies-on-Rat tissues 250 plus slides kit

STAT-Q Rat Adsorbed DAB KIT for staining Mouse ant

NB314RAK-DAB 200-250 slides
EUR 695
Description: STAT-Q Rat Adsorbed DAB KIT for staining Mouse antibodies-on-Rat tissues 250 plus slides kit

ProLite™ His-Tag Protein Gel Staining Kit *Green Fluorescence*

18010 10 Gels
EUR 412

MycoLight™ Rapid Fluorescence Gram-Positive Bacteria Staining Kit

22415 100 Tests
EUR 334

The expression of LC3-II, Beclin-1, and HMGB1 and the ratio of LC3-II / LC3-I increased in H / cardiomyocytes R-processed and subsequently appointed by TMZ pretreatment. However, transfection siHMGB1 exacerbate the effects of H / R in cardiomyocytes and suppresses the protective effects of TMZ on the H / R defective cardiomyocytes with increasing levels of LDH and apoptosis and reduced survival of cardiomyocytes.

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