Background: Following liver injury, mast cells (MC) migrate to the liver and is activated in patients with cholestasis. Inhibition of MC decreased mediator ductular reaction (DR) and liver fibrosis. TGF-β1 contributes to fibrosis and promote liver disease.

Objective: To demonstrate that MC induces reintroduction of cholestatic injury with TGF-β1.

Methods: Wild-type, KitW-sh (MC-deficient), and Mdr2 – / – mice lacking L-histidine decarboxylase injected with vehicle or PKH26-tagged murine MC by treatment with 0.01% DMSO or inhibitor of TGF-βR, LY2109761 (TGF-βRi, 10 M) three days before the bag. liver damage was assessed by H & E and serum chemistry. MC injected detected in the liver, spleen and lungs by immunofluorescence (IF). DR is measured by CK-19 immunohistochemistry (IHC) and F4 / 80 staining coupled with qPCR for IL-1β, IL-33 and F4 / 80; aging bile evaluated by IF or qPCR for p16, p18 and p21. Fibrosis was evaluated by Sirius Red / Fast Green staining and IF to SYP 9, desmin, and α-SMA. secretion of TGF-β1 / expression was measured by EIA and qPCR. Angiogenesis is detected by the IF to vonWillebrand Factor and VEGF-C qPCR. In vitro, MC TGF-β1 expression / secretion was measured after treatment of TGF-βRi; conditioned media is collected. Cholangiocytes and hepatic stellate cell (HSCs) were treated with conditioned medium and bile MC proliferation / aging is measured by MTS and qPCR; HSC activation was evaluated for α-SMA, SYP-9 and expression of collagen type-1a.

Results: MC injection recapitulation cholestatic liver injury is characterized by an increase in DR; fibrosis / TGF-β1 secretion; and angiogenesis. Injection MC-TGF-βRi reverse this parameter. In vitro, induces gall MC proliferation / activation of HSC aging and overturned by MC less TGF-β1.

Conclusion: Our new research shows that the reintroduction of cholestatic liver injury mimics MC and MC derived TGF-β1 may be a target in chronic cholestatic liver disease.

Relieves trimetazidine hypoxia / reoxygenation-induced apoptosis in neonatal rat cardiomyocytes through up-regulating the expression of HMGB1 to promote autophagy

Previous studies have shown the effects of trimetazidine (TMZ) on reducing cardiomyocyte hypoxia / reoxygenation (H / R) injury and the protective effect of autophagy on Ischemia-Reperfusion (I / R) injury to cells. However, whether TMZ protection mechanisms are also involved in autophagy remains unclear.

Our study introduces the role of HMGB1 to check TMZ on autophagy regulation on cardiomyocyte injury H / R. After cell extraction and identification by anti-α-actin staining, cardiomyocytes created hypoxia and reoxygenated, each for 3 hours, and then treated with various concentrations TMZ and transfected with siHMGB1. cell viability and apoptosis were measured by MTS method and flow cytometry, respectively. Autophagy- related factor expression (LC3-I, LC3-II, Beclin-1) and HMGB1 was detected by Western blot and qPCR. Lactate dehydrogenase (LDH) release was assessed by ELISA kit. Cardiomyocytes extracted. H / R lowering of cell viability and increase the level of LDH and cardiomyocyte apoptosis. TMZ had no effect on cardiomyocytes not treated, but reversed the adverse effects of H / R in cardiomyocytes.

Silver Stain kit
AR0171	BosterBio	1 kit (for 30 assays to stain the gel of 5 X8.5)	EUR 152

Silver Phosphate
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GK7224-10G	Glentham Life Sciences	10 g	EUR 70

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GK7224-250G	Glentham Life Sciences	250 g	EUR 373

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GK7224-25G	Glentham Life Sciences	25 g	EUR 94

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GK7224-50G	Glentham Life Sciences	50 g	EUR 142

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GK7224-5G	Glentham Life Sciences	5 g	EUR 56

isa silver
ISA34	Consort	ea	EUR 91

Silver nitrate
SB0479	Bio Basic	25g	EUR 142.22
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DAPI Staining Kit
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63R-SC001	Fitzgerald	100 ml	EUR 511
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Sterling Rapid Silver Stain Kit
NAT1106	National Diagnostics	1KIT	EUR 221

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55R-1541	Fitzgerald	250 assays	EUR 370
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K2035-50	Biovision	50 assays	EUR 379

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2332360	Atto	2unit	EUR 330
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55R-1291	Fitzgerald	25 assays	EUR 282
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55R-1293	Fitzgerald	25 assays	EUR 284
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55R-1294	Fitzgerald	25 assays	EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory

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55R-1295	Fitzgerald	25 assays	EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory

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55R-1296	Fitzgerald	25 assays	EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

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55R-1297	Fitzgerald	25 assays	EUR 296
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StemTAG Alkaline Phosphatase Staining Kit (Red)
CBA-300	Cell Biolabs	100 assays	EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

StemTAG Alkaline Phosphatase Staining Kit (Purple)
CBA-306	Cell Biolabs	100 assays	EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Alcian Blue (pH 2.5) Staining Kit
AFR-1	ScyTek Laboratories	250 ml ea.	EUR 208

Alcian Blue (pH 2.5) Staining Kit
AFR-2	ScyTek Laboratories	30 ml ea.	EUR 128

Lipid (Oil Red O) staining Kit
K580-24	Biovision		EUR 452

Gel-FAST? Gel Staining/Destaining Kit
K901-40	Biovision		EUR 175

CaspGLOW? Fluorescein Active Caspase Staining Kit
K180-100	Biovision		EUR 550

CaspGLOW? Fluorescein Active Caspase Staining Kit
K180-25	Biovision		EUR 234

Fluorescein Active Caspase-12 Staining Kit
K2046-25	ApexBio	25 assays	EUR 238

Fluorescein Active Caspase-2 Staining Kit
K2048-25	ApexBio	25 assays	EUR 238

Fluorescein Active Caspase-3 Staining Kit
K2049-25	ApexBio	25 assays	EUR 238

Fluorescein Active Caspase-8 Staining Kit
K2050-25	ApexBio	25 assays	EUR 238

The expression of LC3-II, Beclin-1, and HMGB1 and the ratio of LC3-II / LC3-I increased in H / cardiomyocytes R-processed and subsequently appointed by TMZ pretreatment. However, transfection siHMGB1 exacerbate the effects of H / R in cardiomyocytes and suppresses the protective effects of TMZ on the H / R defective cardiomyocytes with increasing levels of LDH and apoptosis and reduced survival of cardiomyocytes.