Background: Following liver injury, mast cells (MC) migrate to the liver and is activated in patients with cholestasis. Inhibition of MC decreased mediator ductular reaction (DR) and liver fibrosis. TGF-β1 contributes to fibrosis and promote liver disease.

Objective: To demonstrate that MC induces reintroduction of cholestatic injury with TGF-β1.

Methods: Wild-type, KitW-sh (MC-deficient), and Mdr2 – / – mice lacking L-histidine decarboxylase injected with vehicle or PKH26-tagged murine MC by treatment with 0.01% DMSO or inhibitor of TGF-βR, LY2109761 (TGF-βRi, 10 M) three days before the bag. liver damage was assessed by H & E and serum chemistry. MC injected detected in the liver, spleen and lungs by immunofluorescence (IF). DR is measured by CK-19 immunohistochemistry (IHC) and F4 / 80 staining coupled with qPCR for IL-1β, IL-33 and F4 / 80; aging bile evaluated by IF or qPCR for p16, p18 and p21. Fibrosis was evaluated by Sirius Red / Fast Green staining and IF to SYP 9, desmin, and α-SMA. secretion of TGF-β1 / expression was measured by EIA and qPCR. Angiogenesis is detected by the IF to vonWillebrand Factor and VEGF-C qPCR. In vitro, MC TGF-β1 expression / secretion was measured after treatment of TGF-βRi; conditioned media is collected. Cholangiocytes and hepatic stellate cell (HSCs) were treated with conditioned medium and bile MC proliferation / aging is measured by MTS and qPCR; HSC activation was evaluated for α-SMA, SYP-9 and expression of collagen type-1a.

Results: MC injection recapitulation cholestatic liver injury is characterized by an increase in DR; fibrosis / TGF-β1 secretion; and angiogenesis. Injection MC-TGF-βRi reverse this parameter. In vitro, induces gall MC proliferation / activation of HSC aging and overturned by MC less TGF-β1.

Conclusion: Our new research shows that the reintroduction of cholestatic liver injury mimics MC and MC derived TGF-β1 may be a target in chronic cholestatic liver disease.

Relieves trimetazidine hypoxia / reoxygenation-induced apoptosis in neonatal rat cardiomyocytes through up-regulating the expression of HMGB1 to promote autophagy

Previous studies have shown the effects of trimetazidine (TMZ) on reducing cardiomyocyte hypoxia / reoxygenation (H / R) injury and the protective effect of autophagy on Ischemia-Reperfusion (I / R) injury to cells. However, whether TMZ protection mechanisms are also involved in autophagy remains unclear.

Our study introduces the role of HMGB1 to check TMZ on autophagy regulation on cardiomyocyte injury H / R. After cell extraction and identification by anti-α-actin staining, cardiomyocytes created hypoxia and reoxygenated, each for 3 hours, and then treated with various concentrations TMZ and transfected with siHMGB1. cell viability and apoptosis were measured by MTS method and flow cytometry, respectively. Autophagy- related factor expression (LC3-I, LC3-II, Beclin-1) and HMGB1 was detected by Western blot and qPCR. Lactate dehydrogenase (LDH) release was assessed by ELISA kit. Cardiomyocytes extracted. H / R lowering of cell viability and increase the level of LDH and cardiomyocyte apoptosis. TMZ had no effect on cardiomyocytes not treated, but reversed the adverse effects of H / R in cardiomyocytes.

Silver benzoat
20-abx184359	Abbexa	EUR 258.00 EUR 356.00 EUR 230.00	10 g 25 g 5 g

Silver trifluoromethanesulfonate
20-abx184360	Abbexa	EUR 551.00 EUR 286.00	100 g 25 g

Silver carbonate
20-abx184771	Abbexa	EUR 439.00 EUR 244.00	100 g 25 g

Silver sulfate
GK0139-100G	Glentham Life Sciences	100 g	EUR 301

Silver sulfate
GK0139-25G	Glentham Life Sciences	25 g	EUR 138

Silver sulfadiazine
GK2380-10G	Glentham Life Sciences	10 g	EUR 78

Silver sulfadiazine
GK2380-25G	Glentham Life Sciences	25 g	EUR 126

Silver stearate
GK5303-10G	Glentham Life Sciences	10 g	EUR 363

Silver stearate
GK5303-1G	Glentham Life Sciences	1 g	EUR 94

Silver stearate
GK5303-5G	Glentham Life Sciences	5 g	EUR 221

Silver nitrate
GK7224-100G	Glentham Life Sciences	100 g	EUR 221

Silver nitrate
GK7224-10G	Glentham Life Sciences	10 g	EUR 70

Silver nitrate
GK7224-250G	Glentham Life Sciences	250 g	EUR 373

Silver nitrate
GK7224-25G	Glentham Life Sciences	25 g	EUR 94

Silver nitrate
GK7224-50G	Glentham Life Sciences	50 g	EUR 142

Silver nitrate
GK7224-5G	Glentham Life Sciences	5 g	EUR 56

Silver sulfadiazine
GK2380-5G	Glentham Life Sciences	5 g	EUR 59

Silver methanesulfonate
GX5020-25G	Glentham Life Sciences	25 g	EUR 174

Silver methanesulfonate
GX5020-5G	Glentham Life Sciences	5 g	EUR 71

Silver nitrate
SB0479	Bio Basic	25g	EUR 142.22

isa silver
ISA34	Consort	ea	EUR 91

?-Galactosidase Staining Kit
AKR-100	Cell Biolabs	1 kit	EUR 432
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

DAPI Staining Kit
abx097206-1Kit	Abbexa	1 Kit	EUR 230

PI Staining Kit
abx097208-1Kit	Abbexa	1 Kit	EUR 230

Silver Colloid (20nm)
63R-SC001	Fitzgerald	100 ml	EUR 511
Description: 20nm colloidal silver particles

Silver P-Toluenesulfonate
20-abx186559	Abbexa	EUR 439.00 EUR 230.00	25 g 5 g

Silver homologue Antibody
abx430963-200ul	Abbexa	200 ul	EUR 384

silver electrode, s7
SO65X	Consort	ea	EUR 175

calibration solution silver
ISC34	Consort	ea	EUR 91

silver/sulphide electrode
ISE34B	Consort	ea	EUR 327

Sterling Rapid Silver Stain Kit
NAT1106	National Diagnostics	1KIT	EUR 221

Alkaline Phosphatase Staining Kit
K2035-50	Biovision	50 assays	EUR 379

Beta Galactosidase Staining Kit
55R-1541	Fitzgerald	250 assays	EUR 370
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Beta-Galactosidase Staining Kit
K802-250	Biovision		EUR 294

Human Histochemistry Staining Kit
20-abx299039	Abbexa	EUR 871.00 EUR 1219.00 EUR 565.00	150 tests 500 tests 50 tests

Hoechst 33342 Staining Kit
abx097207-1Kit	Abbexa	1 Kit	EUR 230

Beta-Galactosidase Staining Kit
K2182-250	ApexBio	250 assays	EUR 293

2,4-Pentanedione, Silver Derivative
20-abx186186	Abbexa	EUR 300.00 EUR 1274.00 EUR 578.00	1 g 25 g 5 g

AE-1360 EzStain silver
2332360	Atto	2unit	EUR 330
Description: Si l ver for Pr ot ei n& DN A

Competent Cells, Silver Efficiency
BIO-85026	Bioline	2ml ( 10 x 200µl )	Ask for price

double silver electrode, s7
SP05X	Consort	ea	EUR 233

Silver Nitrate Solution (0.2%)
SNS125	ScyTek Laboratories	125 ml	EUR 72

Silver Nitrate Solution (0.2%)
SNS500	ScyTek Laboratories	500 ml	EUR 119

Silver Nitrate Solution (0.2%)
SNS999	ScyTek Laboratories	1000 ml	EUR 174

Silver Nitrate Solution (5%)
SNV125	ScyTek Laboratories	125 ml	EUR 101

Silver Nitrate Solution (5%)
SNV500	ScyTek Laboratories	500 ml	EUR 231

Silver Nitrate Solution (10%)
SNX009	ScyTek Laboratories	9 ml	EUR 61

Silver Nitrate Solution (10%)
SNX065	ScyTek Laboratories	65 ml	EUR 104

Silver Nitrate Solution (10%)
SNX125	ScyTek Laboratories	125 ml	EUR 149

Silver Nitrate Solution (10%)
SNX500	ScyTek Laboratories	500 ml	EUR 345

Silver Nitrate Solution (1%)
SNY125	ScyTek Laboratories	125 ml	EUR 101

Silver Nitrate Solution (1%)
SNY500	ScyTek Laboratories	500 ml	EUR 172

Frit Kit
FRIT-KIT	Next Advance	1each	EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

CaspGLOW Active Caspase Staining Kit
55R-1292	Fitzgerald	25 assays	EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

Live/Dead Cell Staining Kit
55R-1434	Fitzgerald	100 tests	EUR 638
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Live-Dead Cell Staining Kit
K501-100	Biovision		EUR 468

DNA Ploidy Analysis Staining Kit
K1439-1	Biovision		EUR 588

DNA Ploidy Analysis Staining Kit
K1439-5	Biovision		EUR 2002

Nerve Terminal Staining Kit V
70034	Biotium	1SET	EUR 509
Description: Minimum order quantity: 1 unit of 1SET

Blue-Color AP Staining Kit
AP100B-1	SBI	50 Assays	EUR 262

Dual- Color AP Staining Kit
AP100D-1	SBI	100 Assays	EUR 412

Red-Color AP Staining Kit
AP100R-1	SBI	50 Assays	EUR 262

Fluorescein Active Caspase Staining Kit
K2047-100	ApexBio	100 assays	EUR 572

Fluorescein Active Caspase Staining Kit
K2047-25	ApexBio	25 assays	EUR 238

Red Active Caspase Staining Kit
K2052-100	ApexBio	100 assays	EUR 572

Red Active Caspase Staining Kit
K2052-25	ApexBio	25 assays	EUR 238

Live-Dead Cell Staining Kit
K2081-100	ApexBio	100 staining	EUR 460

Column Packing Kit
PACK-KIT	Next Advance	1pack	EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

silver electrode, dj, 1m, bnc
SO66B	Consort	ea	EUR 214

Silver Nitrate Solution (2%), Acidulated
SNA030	ScyTek Laboratories	30 ml	EUR 88

Silver Nitrate Solution (2%), Acidulated
SNA500	ScyTek Laboratories	500 ml	EUR 303

Silver Nitrate Solution (0.5%), Acidulated
SNB125	ScyTek Laboratories	125 ml	EUR 98

Silver Nitrate Solution (0.5%), Acidulated
SNB500	ScyTek Laboratories	500 ml	EUR 231

PCR Mycoplasma Detection Kit
M034-Kit	TOKU-E	Kit	EUR 266

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit
CAS400A-KIT	SBI	1 kit (10 rxn)	EUR 1110

CaspGLOW Active Caspase 12 Staining Kit
55R-1291	Fitzgerald	25 assays	EUR 282
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit
55R-1293	Fitzgerald	25 assays	EUR 284
Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit
55R-1294	Fitzgerald	25 assays	EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit
55R-1295	Fitzgerald	25 assays	EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 9 Staining Kit
55R-1296	Fitzgerald	25 assays	EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase Staining Kit
55R-1297	Fitzgerald	25 assays	EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

Lipid (Oil Red O) staining Kit
K580-24	Biovision		EUR 452

Gel-FAST? Gel Staining/Destaining Kit
K901-40	Biovision		EUR 175

CaspGLOW? Fluorescein Active Caspase Staining Kit
K180-100	Biovision		EUR 550

CaspGLOW? Fluorescein Active Caspase Staining Kit
K180-25	Biovision		EUR 234

CaspGLOW? Red Active Caspase Staining Kit
K190-100	Biovision		EUR 523

CaspGLOW? Red Active Caspase Staining Kit
K190-25	Biovision		EUR 240

Human Type I Collagen Staining Kit
9044	Chondrex	1 kit	EUR 338.55
Description: Human Type I Collagen Staining Kit

Yeast Viability Staining Kit (Green/Red)
31063-1	Biotium	1kit	EUR 472
Description: Minimum order quantity: 1 unit of 1kit

StemTAG Alkaline Phosphatase Staining Kit (Red)
CBA-300	Cell Biolabs	100 assays	EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

StemTAG Alkaline Phosphatase Staining Kit (Purple)
CBA-306	Cell Biolabs	100 assays	EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Coomassie Brilliant Blue Gel Staining Kit
abx090653-1Kit	Abbexa	1 Kit	EUR 189

Hoechst 33342 / PI Double Staining Kit
abx097209-1Kit	Abbexa	1 Kit	EUR 244

Alcian Blue (pH 2.5) Staining Kit
AFR-1	ScyTek Laboratories	250 ml ea.	EUR 208

Alcian Blue (pH 2.5) Staining Kit
AFR-2	ScyTek Laboratories	30 ml ea.	EUR 128

Fluorescein Active Caspase-12 Staining Kit
K2046-100	ApexBio	100 assays	EUR 572

Fluorescein Active Caspase-12 Staining Kit
K2046-25	ApexBio	25 assays	EUR 238

Fluorescein Active Caspase-2 Staining Kit
K2048-100	ApexBio	100 assays	EUR 572

Fluorescein Active Caspase-2 Staining Kit
K2048-25	ApexBio	25 assays	EUR 238

Fluorescein Active Caspase-3 Staining Kit
K2049-100	ApexBio	100 assays	EUR 572

Fluorescein Active Caspase-3 Staining Kit
K2049-25	ApexBio	25 assays	EUR 238

Fluorescein Active Caspase-8 Staining Kit
K2050-100	ApexBio	100 assays	EUR 572

Fluorescein Active Caspase-8 Staining Kit
K2050-25	ApexBio	25 assays	EUR 238

Fluorescein Active Caspase-9 Staining Kit
K2051-100	ApexBio	100 assays	EUR 572

Fluorescein Active Caspase-9 Staining Kit
K2051-25	ApexBio	25 assays	EUR 238

Red Active Caspase-3 Staining Kit
K2053-100	ApexBio	100 assays	EUR 572

Red Active Caspase-3 Staining Kit
K2053-25	ApexBio	25 assays	EUR 238

Red Active Caspase-8 Staining Kit
K2054-100	ApexBio	100 assays	EUR 572

Red Active Caspase-8 Staining Kit
K2054-25	ApexBio	25 assays	EUR 238

Red Active Caspase-9 Staining Kit
K2055-100	ApexBio	100 assays	EUR 572

Red Active Caspase-9 Staining Kit
K2055-25	ApexBio	25 assays	EUR 238

Human FoxP3 Transcription Factor Staining Kit
OKTA00001	Aviva Systems Biology	KIT	EUR 454
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Human FoxP3 Transcription Factor Staining Kit
OKTA00004	Aviva Systems Biology	KIT	EUR 454
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV100PA-KIT	SBI	1 Kit	EUR 1132

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV105PA-KIT	SBI	1 Kit	EUR 1132

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV120PA-KIT	SBI	1 Kit	EUR 1132

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit
CASLV125PA-KIT	SBI	1 Kit	EUR 1132

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS700A-KIT	SBI	10 rxn	EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS720A-KIT	SBI	10 rxn	EUR 1132

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector
CAS740A-KIT	SBI	10 rxn	EUR 1132

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)
CAS510A-KIT	SBI	1 Kit	EUR 805

Oxidizer Solution (For Steiner's Silver Stain)
SOS125	ScyTek Laboratories	125 ml	EUR 76

Oxidizer Solution (For Steiner's Silver Stain)
SOS500	ScyTek Laboratories	500 ml	EUR 111

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV200PA-KIT	SBI	1 Kit	EUR 1132

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV205PA-KIT	SBI	1 Kit	EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV220PA-KIT	SBI	1 Kit	EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit
CASLV225PA-KIT	SBI	1 Kit	EUR 1132

PI / RNase Staining Buffer
20-abx090619	Abbexa	EUR 272.00 EUR 230.00	100 tests 50 tests

ProsiBlue Gel Staining Solution
P7300-050	GenDepot	500ml	EUR 174

Ponceau S Staining Solution
PW005	Bio Basic	100ml	EUR 69.14

Ponceau S Staining Solution
B7778-100000	ApexBio	100 g	EUR 163

Ponceau S Staining Solution
B7778-50000	ApexBio	50 g	EUR 126

EasyDip Slide Staining System
M900-12AS-ASSORTED	ScyTek Laboratories	1 Case(s)	EUR 176

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS750A-KIT	SBI	10 rxn	EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS770A-KIT	SBI	10 rxn	EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector
CAS790A-KIT	SBI	10 rxn	EUR 1132

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]
CAS9LIG-KIT	SBI	1 Kit	EUR 153

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN320A-KIT	SBI	1 Kit	EUR 4941

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)
PIN340iPS-KIT	SBI	1 Kit	EUR 4941

CaspGLOW Red Active Caspase 3 Staining Kit
55R-1298	Fitzgerald	25 assays	EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 8 Staining Kit
55R-1299	Fitzgerald	25 assays	EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 9 Staining Kit
55R-1300	Fitzgerald	25 assays	EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

EZCell? Intracellular Zinc Staining Kit (Cell-Based)
K525-50	Biovision		EUR 566

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit
K182-100	Biovision		EUR 523

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit
K182-25	Biovision		EUR 234

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit
K183-100	Biovision		EUR 550

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit
K183-25	Biovision		EUR 234

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)
K449-50	Biovision		EUR 544

Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based)
K458-50	Biovision		EUR 572

CaspGLOW? Red Active Caspase-3 Staining Kit
K193-100	Biovision		EUR 533

CaspGLOW? Red Active Caspase-3 Staining Kit
K193-25	Biovision		EUR 240

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit
K188-100	Biovision		EUR 550

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit
K188-25	Biovision		EUR 240

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit
K189-100	Biovision		EUR 550

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit
K189-25	Biovision		EUR 240

CaspGLOW? Red Active Caspase-8 Staining Kit
K198-100	Biovision		EUR 523

CaspGLOW? Red Active Caspase-8 Staining Kit
K198-25	Biovision		EUR 240

CaspGLOW? Red Active Caspase-9 Staining Kit
K199-100	Biovision		EUR 517

CaspGLOW? Red Active Caspase-9 Staining Kit
K199-25	Biovision		EUR 240

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit
K172-100	Biovision		EUR 523

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit
K172-25	Biovision		EUR 234

Type IX Collagen Staining Kit, Multi-Species
9012	Chondrex	1 kit	EUR 338.55
Description: Type IX Collagen Staining Kit

Sirius Red/Fast Green Collagen Staining Kit
9046	Chondrex	30-50 samples	EUR 137
Description: Sirius Red/Fast Green Collagen Staining Kit

Cellular Senescence Detection Kit (SA-?-Gal Staining)
CBA-230	Cell Biolabs	50 assays	EUR 519
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Cellular Senescence Detection Kit (SA-?-Gal Staining)
CBA-230-5	Cell Biolabs	5 x 50 assays	EUR 2051
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Protein Stain-EZ J Phosphoprotein Staining Kit
BSP043	Bio Basic	2x10minigel, 20prep	EUR 110.9

Blue Feulgen DNA Ploidy Analysis Staining Kit
DPK500	ScyTek Laboratories	1 kit(s)	EUR 356

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE620A-KIT	SBI	1 kit	EUR 2132

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE622A-KIT	SBI	1 kit	EUR 2132

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)
GE624A-KIT	SBI	1 kit	EUR 2132

CometAssay Single Cell Gel Electrophoresis Assay-Silver
4251-050-K	Biotechne	1 kit	EUR 570.8

Coomassie Blue Staining Destaining Solution
AR0140	BosterBio	1kit (for 10 assays to stain gels of 5 x 8.5cm)	EUR 70

Coomassie Blue Fast Staining Solution
AR0170	BosterBio	100mL(for 10 assays to stain gels of 5 x 8.5cm)	EUR 80

7-AAD Viability Staining Buffer
abx090617-100tests	Abbexa	100 tests	EUR 217

SafePinky DNA Gel Staining Solution
S1000-050	GenDepot	500ml	EUR 131

SafePinky DNA Gel Staining Solution
S1000-100	GenDepot	2X500ml	EUR 188

SafePinky DNA Gel Staining Solution
S1000-200	GenDepot	4X500ml	EUR 280

7-AAD Viability Staining Solution
EXB0026	ExBio	400 tests	EUR 76

Manual Staining System (12 Wells)
IMS001	ScyTek Laboratories	1 ea.	EUR 356

Stain Tray Slide Staining System
M920-1	ScyTek Laboratories	1 Clear	EUR 428

Stain Tray Slide Staining System
M920-2	ScyTek Laboratories	1 Black	EUR 445

vWF Acty. Kit
ABP-ACT-KIT	Abpcorp	12 x 8 microwells	EUR 428

vWF Ant. Kit
ABP-TOT-KIT	Abpcorp	12 x 8 microwells	EUR 394

Gelite™ Green Nucleic Acid Gel Staining Kit
17589	AAT Bioquest	1 Kit	EUR 219

Gelite™ Orange Nucleic Acid Gel Staining Kit
17594	AAT Bioquest	1 Kit	EUR 219

Cell Navigator™ Lysosome Staining Kit *NIR Fluorescence*
22652	AAT Bioquest	500 Tests	EUR 219

Cell Navigator™ Lysosome Staining Kit *Blue Fluorescence*
22655	AAT Bioquest	500 Tests	EUR 176

Cell Navigator™ Lysosome Staining Kit *Green Fluorescence*
22656	AAT Bioquest	500 Tests	EUR 176

Cell Navigator™ Lysosome Staining Kit *Orange Fluorescence*
22657	AAT Bioquest	500 Tests	EUR 176

Cell Navigator™ Lysosome Staining Kit *Red Fluorescence*
22658	AAT Bioquest	500 Tests	EUR 176

The expression of LC3-II, Beclin-1, and HMGB1 and the ratio of LC3-II / LC3-I increased in H / cardiomyocytes R-processed and subsequently appointed by TMZ pretreatment. However, transfection siHMGB1 exacerbate the effects of H / R in cardiomyocytes and suppresses the protective effects of TMZ on the H / R defective cardiomyocytes with increasing levels of LDH and apoptosis and reduced survival of cardiomyocytes.