Background: GATA binding protein 3 (GATA3) and miR-29b associated with colorectal cancer (CRC). The current study explored the relationship between GATA3 and regulation of miR-29b, and the mechanism of drug resistance two in CRC cells to oxaliplatin.
Methods: Apoptosis CRC cells caused by oxaliplatin in various doses was detected by flow cytometry. CRC cells separately transfected with excess and knockdown of GATA3, agomir miR-29b and antagomir, and treated by oxaliplatin for the detection of cell viability and apoptosis by cell couting Kit-8 (CCK-8) and flow cytometry. The expression levels of GATA3, caspase3 and cleaved caspase3 determined by Western blot, and the expression of miR-29b was detected by real-time quantitative polymerase chain reaction (qRT-PCR). animal experiments were conducted to test the changes of transplanted tumor xenografts in nude mice studies and observed by in vivo imaging. TUNEL staining performed to detect apoptosis of tumor cells.
Results: Both GATA3 and miR-29b agomir CRC inhibit the activity of cells, apoptosis is promoted and Cleaved caspase3 expression, and reduces cell resistance to chemotherapy drug oxaliplatin. Although GATA3 can up-regulate the expression of miR-29b, a tumor-suppression effects of GATA3 partially reversed by miR-29b antagomir. In vivo experiments showed that down-regulates expression of GATA3 promoted and the growth rate of transplanted tumor volume, while expressing GATA3 no significant effect on tumor growth. TUNEL staining results showed that knock down or overexpression of GATA3 not cause significant changes in the body of CRC cell apoptosis, while oxaliplatin treatment increases the number of apoptotic bodies.
Conclusion: GATA3 inhibit cell survival of CRC cells, promote apoptosis, and reduce oxaliplatin resistance of CRC cells through regulate miR-29b.
Overexpressed GATA3 enhances the sensitivity of colorectal cancer cells to oxaliplatin through regulating MiR-29b
Chloroquine Overcome Imatinib Resistance Combined with Imatinib in Gastrointestinal Stromal Tumors by Inhibiting Autophagy through the MAPK / ERK Pathway
Background: Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms of the gastrointestinal tract. However, up to 40-50% of GISTs develop secondary resistance after a median of 24 months of imatinib treatment. It has been reported that autophagy can promote cell survival and induces drug resistance GIST. Currently, the specific mechanism of autophagy in GISTs with imatinib resistance is not clear.
Materials and Methods: Cell-counting kit (CCK) -8 and flow cytometry method used for in vitro drug sensitivity testing and detection levels of autophagy. The detection rate of apoptosis is by flow cytometry with annexin V Kit. Western blotting was used to analyze the role of autophagy and apoptosis in GIST cells with CQ alone, imatinib alone, or in combination, and to analyze the expression of MAPK pathway.
In vitro results were confirmed by in vivo experiments using mouse models. Hematoxylin and eosin and immunohistochemical staining is used to detect pathological characteristics and immunophenotype transplanted tumor. Detection of KIT and PDGFRA gene mutations in imatinib-resistant GIST transplant performed by denaturing high-performance liquid chromatography (DHPLC) and direct sequencing. ERK and KIT expression and regulation of the level detected by Western blotting.
Description: A competitive ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A Sandwich ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A competitive ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Results: In vitro and in vivo experiments, the level of imatinib-resistant cell autophagy higher than normal cells; CQ combined with imatinib may promote apoptosis by blocking the imatinib-resistant cell autophagy. Meanwhile, we found that the levels of ERK phosphorylation may be associated with autophagy.
Gina
