Overexpressed GATA3 enhances the sensitivity of colorectal cancer cells to oxaliplatin through regulating MiR-29b

Overexpressed GATA3 enhances the sensitivity of colorectal cancer cells to oxaliplatin through regulating MiR-29b

Background: GATA binding protein 3 (GATA3) and miR-29b associated with colorectal cancer (CRC). The current study explored the relationship between GATA3 and regulation of miR-29b, and the mechanism of drug resistance two in CRC cells to oxaliplatin.


Methods: Apoptosis CRC cells caused by oxaliplatin in various doses was detected by flow cytometry. CRC cells separately transfected with excess and knockdown of GATA3, agomir miR-29b and antagomir, and treated by oxaliplatin for the detection of cell viability and apoptosis by cell couting Kit-8 (CCK-8) and flow cytometry. The expression levels of GATA3, caspase3 and cleaved caspase3 determined by Western blot, and the expression of miR-29b was detected by real-time quantitative polymerase chain reaction (qRT-PCR). animal experiments were conducted to test the changes of transplanted tumor xenografts in nude mice studies and observed by in vivo imaging. TUNEL staining performed to detect apoptosis of tumor cells.


Results:
Both GATA3 and miR-29b agomir CRC inhibit the activity of cells, apoptosis is promoted and Cleaved caspase3 expression, and reduces cell resistance to chemotherapy drug oxaliplatin. Although GATA3 can up-regulate the expression of miR-29b, a tumor-suppression effects of GATA3 partially reversed by miR-29b antagomir. In vivo experiments showed that down-regulates expression of GATA3 promoted and the growth rate of transplanted tumor volume, while expressing GATA3 no significant effect on tumor growth. TUNEL staining results showed that knock down or overexpression of GATA3 not cause significant changes in the body of CRC cell apoptosis, while oxaliplatin treatment increases the number of apoptotic bodies.


Conclusion: GATA3 inhibit cell survival of CRC cells, promote apoptosis, and reduce oxaliplatin resistance of CRC cells through regulate miR-29b.

 Overexpressed GATA3 enhances the sensitivity of colorectal cancer cells to oxaliplatin through regulating MiR-29b
Overexpressed GATA3 enhances the sensitivity of colorectal cancer cells to oxaliplatin through regulating MiR-29b

Chloroquine Overcome Imatinib Resistance Combined with Imatinib in Gastrointestinal Stromal Tumors by Inhibiting Autophagy through the MAPK / ERK Pathway

Background: Gastrointestinal stromal tumors (GISTs) are the most common mesenchymal neoplasms of the gastrointestinal tract. However, up to 40-50% of GISTs develop secondary resistance after a median of 24 months of imatinib treatment. It has been reported that autophagy can promote cell survival and induces drug resistance GIST. Currently, the specific mechanism of autophagy in GISTs with imatinib resistance is not clear.


Materials and Methods: Cell-counting kit (CCK) -8 and flow cytometry method used for in vitro drug sensitivity testing and detection levels of autophagy. The detection rate of apoptosis is by flow cytometry with annexin V Kit. Western blotting was used to analyze the role of autophagy and apoptosis in GIST cells with CQ alone, imatinib alone, or in combination, and to analyze the expression of MAPK pathway.

In vitro results were confirmed by in vivo experiments using mouse models. Hematoxylin and eosin and immunohistochemical staining is used to detect pathological characteristics and immunophenotype transplanted tumor. Detection of KIT and PDGFRA gene mutations in imatinib-resistant GIST transplant performed by denaturing high-performance liquid chromatography (DHPLC) and direct sequencing. ERK and KIT expression and regulation of the level detected by Western blotting.

Collagen Type IV protein

E62L004 1mg
EUR 1425.6

Collagen Type IV antibody

10R-7847 100 ug
EUR 386.4
Description: Mouse monoclonal Collagen Type IV antibody

Collagen Type IV antibody

10R-7977 100 ug
EUR 567.6
Description: Mouse monoclonal Collagen Type IV antibody

Collagen Type IV antibody

10R-C137a 1 mL
EUR 640.8
Description: Mouse monoclonal Collagen Type IV antibody

Collagen Type IV antibody

20R-CR024 250 uL
EUR 633.6
Description: Rabbit polyclonal Collagen Type IV antibody

Collagen Type IV antibody

70R-CR013x 100 µg
EUR 403.2
Description: Affinity purified Rabbit polyclonal Collagen Type IV antibody

Collagen Type IV Antibody

P1017-01m 0.1m
EUR 242.4

Collagen Type IV Antibody

P1017-1ml 1ml
EUR 685.2

Collagen Type IV antibody

CAF50522-100ug 100ug
EUR 312

Rat Collagen Type IV ELISA kit

E02C0047-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collagen Type IV ELISA kit

E02C0047-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collagen Type IV ELISA kit

E02C0047-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collagen Type IV ELISA kit

E02C0780-192T 192 tests
EUR 1524
Description: A Sandwich ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collagen Type IV ELISA kit

E02C0780-48 1 plate of 48 wells
EUR 624
Description: A Sandwich ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Collagen Type IV ELISA kit

E02C0780-96 1 plate of 96 wells
EUR 822
Description: A Sandwich ELISA for quantitative measurement of Rat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Collagen Type IV ELISA kit

E08C0780-192T 192 tests
EUR 1524
Description: A Sandwich ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Collagen Type IV ELISA kit

E08C0780-48 1 plate of 48 wells
EUR 624
Description: A Sandwich ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Collagen Type IV ELISA kit

E08C0780-96 1 plate of 96 wells
EUR 822
Description: A Sandwich ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Collagen Type IV ELISA kit

E08C0047-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Collagen Type IV ELISA kit

E08C0047-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Collagen Type IV ELISA kit

E08C0047-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Canine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Collagen Type IV ELISA kit

E07C0047-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Collagen Type IV ELISA kit

E07C0047-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Collagen Type IV ELISA kit

E07C0047-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Collagen Type IV ELISA kit

E07C0780-192T 192 tests
EUR 1524
Description: A Sandwich ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Collagen Type IV ELISA kit

E07C0780-48 1 plate of 48 wells
EUR 624
Description: A Sandwich ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Collagen Type IV ELISA kit

E07C0780-96 1 plate of 96 wells
EUR 822
Description: A Sandwich ELISA for quantitative measurement of Porcine Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Collagen Type IV ELISA kit

E06C0780-192T 192 tests
EUR 1524
Description: A Sandwich ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Collagen Type IV ELISA kit

E06C0780-48 1 plate of 48 wells
EUR 624
Description: A Sandwich ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Collagen Type IV ELISA kit

E06C0780-96 1 plate of 96 wells
EUR 822
Description: A Sandwich ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Collagen Type IV ELISA kit

E06C0047-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Collagen Type IV ELISA kit

E06C0047-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Collagen Type IV ELISA kit

E06C0047-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Goat Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Collagen Type IV ELISA kit

E01C0047-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Human Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Collagen Type IV ELISA kit

E01C0047-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Human Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Collagen Type IV ELISA kit

E01C0047-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Human Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Collagen Type IV ELISA kit

E03C0047-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Mouse Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Collagen Type IV ELISA kit

E03C0047-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Mouse Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Collagen Type IV ELISA kit

E03C0047-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Mouse Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Collagen Type IV ELISA kit

E01C0780-192T 192 tests
EUR 1524
Description: A Sandwich ELISA for quantitative measurement of Human Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Collagen Type IV ELISA kit

E01C0780-48 1 plate of 48 wells
EUR 624
Description: A Sandwich ELISA for quantitative measurement of Human Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Collagen Type IV ELISA kit

E01C0780-96 1 plate of 96 wells
EUR 822
Description: A Sandwich ELISA for quantitative measurement of Human Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Collagen Type IV ELISA kit

E03C0780-192T 192 tests
EUR 1524
Description: A Sandwich ELISA for quantitative measurement of Mouse Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Collagen Type IV ELISA kit

E03C0780-48 1 plate of 48 wells
EUR 624
Description: A Sandwich ELISA for quantitative measurement of Mouse Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Collagen Type IV ELISA kit

E03C0780-96 1 plate of 96 wells
EUR 822
Description: A Sandwich ELISA for quantitative measurement of Mouse Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Collagen Type IV ELISA KIT

ELA-E0180m 96 Tests
EUR 1038

Rabbit Collagen Type IV ELISA kit

E04C0780-192T 192 tests
EUR 1524
Description: A Sandwich ELISA for quantitative measurement of Rabbit Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Collagen Type IV ELISA kit

E04C0780-48 1 plate of 48 wells
EUR 624
Description: A Sandwich ELISA for quantitative measurement of Rabbit Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Collagen Type IV ELISA kit

E04C0780-96 1 plate of 96 wells
EUR 822
Description: A Sandwich ELISA for quantitative measurement of Rabbit Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Collagen Type IV ELISA kit

E04C0047-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Rabbit Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Collagen Type IV ELISA kit

E04C0047-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Rabbit Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Collagen Type IV ELISA kit

E04C0047-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Rabbit Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Collagen Type IV ELISA kit

E09C0047-192T 192 tests
EUR 1524
Description: A competitive ELISA for quantitative measurement of Monkey Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Collagen Type IV ELISA kit

E09C0047-48 1 plate of 48 wells
EUR 624
Description: A competitive ELISA for quantitative measurement of Monkey Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Collagen Type IV ELISA kit

E09C0047-96 1 plate of 96 wells
EUR 822
Description: A competitive ELISA for quantitative measurement of Monkey Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Collagen Type IV ELISA kit

E09C0780-192T 192 tests
EUR 1524
Description: A Sandwich ELISA for quantitative measurement of Monkey Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Collagen Type IV ELISA kit

E09C0780-48 1 plate of 48 wells
EUR 624
Description: A Sandwich ELISA for quantitative measurement of Monkey Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Collagen Type IV ELISA kit

E09C0780-96 1 plate of 96 wells
EUR 822
Description: A Sandwich ELISA for quantitative measurement of Monkey Collagen Type IV in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Collagen Type IV ELISA Kit

ELA-E0180Mo 96 Tests
EUR 1113.6

Rat Collagen Type IV (COL4) CLIA Kit

abx196379-96tests 96 tests
EUR 990

Rat Collagen Type IV (COL4) CLIA Kit

20-abx491198
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Pig Collagen Type IV (COL4) CLIA Kit

20-abx496628
  • EUR 10282.80
  • EUR 5472.00
  • EUR 1262.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Collagen Type IV (COL4) Antibody

abx231840-100ug 100 ug
EUR 577.2

Collagen Type IV (COL4) Antibody

abx231841-100ug 100 ug
EUR 577.2

Collagen Type IV (COL4) Antibody

abx231842-100ug 100 ug
EUR 610.8

Collagen Type IV (COL4) Antibody

abx231843-100ug 100 ug
EUR 577.2

Collagen Type IV (COL4) Antibody

abx231844-100ug 100 ug
EUR 577.2

Collagen Type IV (COL4) Antibody

abx411164-02mg 0.2 mg
EUR 994.8

Collagen Type IV (COL4) Antibody

abx411243-1ml 1 ml
EUR 794.4

Collagen Type IV (COL4) Antibody

abx411244-05ml 0.5 ml
EUR 844.8

Collagen Type IV (COL4) Antibody

abx411248-01ml 0.1 ml
EUR 844.8

Collagen Type IV (COL4) Antibody

abx020884-1ml 1 ml
EUR 1220.4

Collagen Type IV (COL4) Antibody

abx022754-05ml 0.5 ml
EUR 1328.4

Collagen Type IV (COL4) Antibody

abx022755-05ml 0.5 ml
EUR 1713.6

Collagen Type IV (COL4) Antibody

abx022756-05ml 0.5 ml
EUR 1554

Collagen Type IV (COL4) Antibody

abx022758-02mg 0.2 mg
EUR 1545.6

Collagen Type IV (COL4) Antibody

20-abx101468
  • EUR 477.60
  • EUR 159.60
  • EUR 1328.40
  • EUR 644.40
  • EUR 376.80
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug

Rat Collagen Type IV (COL4) ELISA Kit

abx256822-96tests 96 tests
EUR 904.8

Rat Collagen Type IV (COL4) ELISA Kit

20-abx155378
  • EUR 8684.40
  • EUR 4626.00
  • EUR 1074.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Pig Collagen Type IV (COL4) ELISA Kit

abx361367-96tests 96 tests
EUR 990

Pig Collagen Type IV (COL4) ELISA Kit

20-abx585246
  • EUR 9174.00
  • EUR 4888.80
  • EUR 1130.40
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Rat Collagen Type IV ELISA Kit (COL4)

RK03583 96 Tests
EUR 625.2

for Collagen Type IV (COL4)ELISA kit

SEA180Po-10x96wellstestplate 10x96-wells test plate
EUR 6117.62
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Collagen Type IV (COL4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

for Collagen Type IV (COL4)ELISA kit

SEA180Po-1x48wellstestplate 1x48-wells test plate
EUR 608.98
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Collagen Type IV (COL4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

for Collagen Type IV (COL4)ELISA kit

SEA180Po-1x96wellstestplate 1x96-wells test plate
EUR 818.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Collagen Type IV (COL4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

for Collagen Type IV (COL4)ELISA kit

SEA180Po-5x96wellstestplate 5x96-wells test plate
EUR 3323.45
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of for Collagen Type IV (COL4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

ELISA Kit for Collagen Type IV (COL4)

4-SEA180Po
  • EUR 6178.80
  • EUR 3264.00
  • EUR 819.60
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Collagen Type IV (COL4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Collagen Type IV (COL4) ELISA Kit

SEA180Ra-10x96wellstestplate 10x96-wells test plate
EUR 5552.14
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Collagen Type IV (COL4) in serum, plasma, tissue homogenates and other biological fluids.

Rat Collagen Type IV (COL4) ELISA Kit

SEA180Ra-1x48wellstestplate 1x48-wells test plate
EUR 562.42
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Collagen Type IV (COL4) in serum, plasma, tissue homogenates and other biological fluids.

Rat Collagen Type IV (COL4) ELISA Kit

SEA180Ra-1x96wellstestplate 1x96-wells test plate
EUR 752.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Collagen Type IV (COL4) in serum, plasma, tissue homogenates and other biological fluids.

Rat Collagen Type IV (COL4) ELISA Kit

SEA180Ra-5x96wellstestplate 5x96-wells test plate
EUR 3024.07
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Collagen Type IV (COL4) in serum, plasma, tissue homogenates and other biological fluids.

Rat Collagen Type IV (COL4) ELISA Kit

4-SEA180Ra
  • EUR 5612.40
  • EUR 2965.20
  • EUR 752.40
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Collagen Type IV (COL4) in samples from serum, plasma, tissue homogenates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat Collagen Type IV (COL4) ELISA Kit

EKN44434-48T 48T
EUR 370.02

Rat Collagen Type IV (COL4) ELISA Kit

EKN44434-5x96T 5x96T
EUR 2510.85

Rat Collagen Type IV (COL4) ELISA Kit

EKN44434-96T 96T
EUR 528.6

Rat Collagen Type IV (COL4) ELISA Kit

EKU03323-48T 48T
EUR 542.78

Rat Collagen Type IV (COL4) ELISA Kit

EKU03323-5x96T 5x96T
EUR 3683.15

Rat Collagen Type IV (COL4) ELISA Kit

EKU03323-96T 96T
EUR 775.4

Pig Collagen Type IV (COL4) ELISA Kit

EKN51846-48T 48T
EUR 373.38

Pig Collagen Type IV (COL4) ELISA Kit

EKN51846-5x96T 5x96T
EUR 2533.65

Pig Collagen Type IV (COL4) ELISA Kit

EKN51846-96T 96T
EUR 533.4

Pig Collagen Type IV (COL4) ELISA Kit

EKU10735-48T 48T
EUR 600.25

Pig Collagen Type IV (COL4) ELISA Kit

EKU10735-5x96T 5x96T
EUR 4073.13

Pig Collagen Type IV (COL4) ELISA Kit

EKU10735-96T 96T
EUR 857.5

Rat Collagen Type IV (COL4) ELISA Kit

NSL1551r 96 Tests
EUR 498

Rat COL4(Collagen Type IV) ELISA Kit

ELK1712-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Rat COL4(Collagen Type IV) ELISA Kit

ELK1712-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat COL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Collagen Type IV IHC Antibody

IW-MA1030 -
EUR 285

Human Collagen Type IV (COL4) CLIA Kit

abx196377-96tests 96 tests
EUR 990

Mouse Collagen Type IV (COL4) CLIA Kit

abx196378-96tests 96 tests
EUR 990

Human Collagen Type IV (COL4) CLIA Kit

20-abx190890
  • EUR 9493.20
  • EUR 5058.00
  • EUR 1167.60
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Collagen Type IV (COL4) CLIA Kit

20-abx491197
  • EUR 9567.60
  • EUR 5095.20
  • EUR 1177.20
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human Collagen Type IV (COL4)CLIA Kit

SCA180Hu-10x96wellstestplate 10x96-wells test plate
EUR 6777.36
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Collagen Type IV (COL4) in serum, plasma, tissue homogenates and other biological fluids.

Human Collagen Type IV (COL4)CLIA Kit

SCA180Hu-1x48wellstestplate 1x48-wells test plate
EUR 663.31
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Collagen Type IV (COL4) in serum, plasma, tissue homogenates and other biological fluids.

Human Collagen Type IV (COL4)CLIA Kit

SCA180Hu-1x96wellstestplate 1x96-wells test plate
EUR 896.16
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Collagen Type IV (COL4) in serum, plasma, tissue homogenates and other biological fluids.

Human Collagen Type IV (COL4)CLIA Kit

SCA180Hu-5x96wellstestplate 5x96-wells test plate
EUR 3672.72
Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Collagen Type IV (COL4) in serum, plasma, tissue homogenates and other biological fluids.

Human Collagen Type IV (COL4) CLIA Kit

4-SCA180Hu
  • EUR 6837.60
  • EUR 3673.20
  • EUR 896.40
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Double-antibody Sandwich chemiluminescent immunoassay for detection of Human Collagen Type IV (COL4)Serum, plasma, tissue homogenates and other biological fluids

Human Collagen Type IV (COL4) CLIA Kit

EKU08857-48T 48T
EUR 666.82

Human Collagen Type IV (COL4) CLIA Kit

EKU08857-5x96T 5x96T
EUR 4524.85

Human Collagen Type IV (COL4) CLIA Kit

EKU08857-96T 96T
EUR 952.6

Human Collagen Type IV (COL4) CLIA Kit

EKN49968-48T 48T
EUR 414.89

Human Collagen Type IV (COL4) CLIA Kit

EKN49968-5x96T 5x96T
EUR 2815.33

Human Collagen Type IV (COL4) CLIA Kit

EKN49968-96T 96T
EUR 592.7

Goat Collagen Type IV(COL4) ELISA Kit

NSL0896Gt 96T
EUR 528

Human Collagen Type IV Protein

abx060774-05mg 0.5 mg
EUR 1496.4

anti- Collagen Type IV antibody

FNab01844 100µg
EUR 606.3
Description: Antibody raised against Collagen Type IV

Collagen Type IV (COL4A1) Antibody

20-abx013045
  • EUR 376.80
  • EUR 117.60
  • EUR 477.60
  • EUR 594.00
  • 100 ug
  • 10 ug
  • 200 ug
  • 300 µg

Human Collagen Type IV (COL4) ELISA Kit

20-abx151127
  • EUR 7970.40
  • EUR 4250.40
  • EUR 990.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Mouse Collagen Type IV (COL4) ELISA Kit

abx255284-96tests 96 tests
EUR 848.4

Human Collagen Type IV (COL4) ELISA Kit

abx252254-96tests 96 tests
EUR 848.4

Sheep Collagen Type IV (COL4) ELISA Kit

abx364200-96tests 96 tests
EUR 1111.2

Mouse Collagen Type IV (COL4) ELISA Kit

20-abx153851
  • EUR 7970.40
  • EUR 4250.40
  • EUR 990.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests

Human COL4(Collagen Type IV) ELISA Kit

EH2867 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Homo sapiens;Sensitivity: 0.469 ng/ml

Mouse COL4(Collagen Type IV) ELISA Kit

EM0944 96T
EUR 628.92
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Mus ;Sensitivity: 0.938 ng/ml

Human Collagen Type IV ELISA Kit (COL4)

RK01155 96 Tests
EUR 625.2

Mouse Collagen Type IV ELISA Kit (COL4)

RK02700 96 Tests
EUR 625.2

Human Collagen Type IV (COL4) ELISA Kit

SEA180Hu-10x96wellstestplate 10x96-wells test plate
EUR 5128.02
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type IV (COL4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Collagen Type IV (COL4) ELISA Kit

SEA180Hu-1x48wellstestplate 1x48-wells test plate
EUR 527.48
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type IV (COL4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Collagen Type IV (COL4) ELISA Kit

SEA180Hu-1x96wellstestplate 1x96-wells test plate
EUR 702.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type IV (COL4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Collagen Type IV (COL4) ELISA Kit

SEA180Hu-5x96wellstestplate 5x96-wells test plate
EUR 2799.54
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Human Collagen Type IV (COL4) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Human Collagen Type IV (COL4) ELISA Kit

4-SEA180Hu
  • EUR 5188.80
  • EUR 2739.60
  • EUR 703.20
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Human Collagen Type IV (COL4) in samples from serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids with no significant corss-reactivity with analogues from other species.

Mouse Collagen Type IV (COL4) ELISA Kit

SEA180Mu-10x96wellstestplate 10x96-wells test plate
EUR 5269.39
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Collagen Type IV (COL4) in serum, plasma and other biological fluids.

Mouse Collagen Type IV (COL4) ELISA Kit

SEA180Mu-1x48wellstestplate 1x48-wells test plate
EUR 539.12
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Collagen Type IV (COL4) in serum, plasma and other biological fluids.

Mouse Collagen Type IV (COL4) ELISA Kit

SEA180Mu-1x96wellstestplate 1x96-wells test plate
EUR 718.75
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Collagen Type IV (COL4) in serum, plasma and other biological fluids.

Mouse Collagen Type IV (COL4) ELISA Kit

SEA180Mu-5x96wellstestplate 5x96-wells test plate
EUR 2874.38
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Collagen Type IV (COL4) in serum, plasma and other biological fluids.

Mouse Collagen Type IV (COL4) ELISA Kit

4-SEA180Mu
  • EUR 5330.40
  • EUR 2815.20
  • EUR 718.80
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Collagen Type IV (COL4) in samples from serum, plasma and other biological fluids with no significant corss-reactivity with analogues from other species.

Human Collagen Type IV (COL4) ELISA Kit

EKN44432-48T 48T
EUR 341.04

Human Collagen Type IV (COL4) ELISA Kit

EKN44432-5x96T 5x96T
EUR 2314.2

Human Collagen Type IV (COL4) ELISA Kit

EKN44432-96T 96T
EUR 487.2

Mouse Collagen Type IV (COL4) ELISA Kit

EKN44433-48T 48T
EUR 350.42

Mouse Collagen Type IV (COL4) ELISA Kit

EKN44433-5x96T 5x96T
EUR 2377.85

Mouse Collagen Type IV (COL4) ELISA Kit

EKN44433-96T 96T
EUR 500.6

Human COL4(Collagen Type IV) ELISA Kit

EKF57575-48T 48T
EUR 396.9

Human COL4(Collagen Type IV) ELISA Kit

EKF57575-5x96T 5x96T
EUR 2693.25

Human COL4(Collagen Type IV) ELISA Kit

EKF57575-96T 96T
EUR 567

Human Collagen Type IV (COL4) ELISA Kit

EKU03321-48T 48T
EUR 500.5

Human Collagen Type IV (COL4) ELISA Kit

EKU03321-5x96T 5x96T
EUR 3396.25

Human Collagen Type IV (COL4) ELISA Kit

EKU03321-96T 96T
EUR 715

Mouse Collagen Type IV (COL4) ELISA Kit

EKU03322-48T 48T
EUR 514.08

Mouse Collagen Type IV (COL4) ELISA Kit

EKU03322-5x96T 5x96T
EUR 3488.4

Mouse Collagen Type IV (COL4) ELISA Kit

EKU03322-96T 96T
EUR 734.4

Mouse COL4(Collagen Type IV) ELISA Kit

EKF58219-48T 48T
EUR 396.9

Mouse COL4(Collagen Type IV) ELISA Kit

EKF58219-5x96T 5x96T
EUR 2693.25

Mouse COL4(Collagen Type IV) ELISA Kit

EKF58219-96T 96T
EUR 567

Human Collagen Type IV (COL4) ELISA Kit

EKU11878-48T 48T
EUR 550.34

Human Collagen Type IV (COL4) ELISA Kit

EKU11878-5x96T 5x96T
EUR 3734.45

Human Collagen Type IV (COL4) ELISA Kit

EKU11878-96T 96T
EUR 786.2

Human Collagen Type IV (COL4) ELISA Kit

EKN52989-48T 48T
EUR 342.3

Human Collagen Type IV (COL4) ELISA Kit

EKN52989-5x96T 5x96T
EUR 2322.75

Human Collagen Type IV (COL4) ELISA Kit

EKN52989-96T 96T
EUR 489

Human Collagen Type IV (COL4)ELISA Kit

NSL0065Hu 96 wells
EUR 468

Mouse Collagen Type IV (COL4) ELISA Kit

NSL1716m 96 Test
EUR 528

Mouse COL4(Collagen Type IV) ELISA Kit

ELK1665-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse COL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Mouse COL4(Collagen Type IV) ELISA Kit

ELK1665-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse COL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human COL4(Collagen Type IV) ELISA Kit

ELK2331-48T 48T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human COL4(Collagen Type IV) ELISA Kit

ELK2331-96T 96T Ask for price
Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL4 in the samples is then determined by comparing the OD of the samples to the standard curve.

Human Type-IV collagen ELISA Kit

DEIA1122 96T
EUR 992.4
Description: The Human Type-IV collagen ELISA kit is designed to detect and quantify the level of human Type-IV collagen in serum.

Rabbit Collagen Type IV (COL4) ELISA Kit

abx363419-96tests 96 tests
EUR 990

Monkey Collagen Type IV (COL4) ELISA Kit

abx359585-96tests 96 tests
EUR 990

Rabbit Collagen Type IV (COL4) ELISA Kit

EKU11877-48T 48T
EUR 542.78

Rabbit Collagen Type IV (COL4) ELISA Kit

EKU11877-5x96T 5x96T
EUR 3683.15

Rabbit Collagen Type IV (COL4) ELISA Kit

EKU11877-96T 96T
EUR 775.4

Bovine Collagen Type IV (COL4) ELISA Kit

EKU11879-48T 48T
EUR 600.25

Bovine Collagen Type IV (COL4) ELISA Kit

EKU11879-5x96T 5x96T
EUR 4073.13

Bovine Collagen Type IV (COL4) ELISA Kit

EKU11879-96T 96T
EUR 857.5

Rabbit Collagen Type IV (COL4) ELISA Kit

EKN52988-48T 48T
EUR 337.82

Rabbit Collagen Type IV (COL4) ELISA Kit

EKN52988-5x96T 5x96T
EUR 2292.35

Rabbit Collagen Type IV (COL4) ELISA Kit

EKN52988-96T 96T
EUR 482.6

Bovine Collagen Type IV (COL4) ELISA Kit

EKN52990-48T 48T
EUR 373.38

Bovine Collagen Type IV (COL4) ELISA Kit

EKN52990-5x96T 5x96T
EUR 2533.65


Results
: In vitro and in vivo experiments, the level of imatinib-resistant cell autophagy higher than normal cells; CQ combined with imatinib may promote apoptosis by blocking the imatinib-resistant cell autophagy. Meanwhile, we found that the levels of ERK phosphorylation may be associated with autophagy.

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