Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma

Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma

Objective: LRRC59 (leucine-rich repeat-containing protein 59) is a ribosome-binding protein which also interacts with the fibroblast growth factor. limited investigation reveals the possibility of LRRC59 role in aggressive phenotype of breast cancer. However, if LRRC59 contribute to the development of lung cancer is still unclear.


Materials and Methods: In this study, a software-based survival analysis TCGA online (GEPIA2) was used to estimate the prognostic value LRRC59 mRNA expression levels for lung cancer. Cells Counting Kit-8 assay, colony-forming assay, cell cycle analysis, and transwell test used to assess the biological function LRRC59 in lung cancer cells. Then, 94 lung adenocarcinoma (LUAD) patient tissue were collected to test the level LRRC59 expression by tissue microarray (TMA) based immunohistochemical staining (IHC). Kaplan-Meier univariate and multivariate Cox regression analysis was performed to evaluate the prognostic value of protein expression in LUAD LRRC59.


Results: High Level of LRRC59 mRNA was significantly associated with poorer survival for lung adenocarcinoma, but not for lung squamous cell carcinoma. ShRNA knockdown of LRRC59 by apparently inhibiting cell proliferation and colony formation in both H1299 and A549 cells. G1 / S phase arrest caused by depletion LRRC59 observed in A549 and H1299 cells. In addition, silencing LRRC59 decreased migrative cells and invasive ability. In addition, the TMA-based IHC showed that it was highly expressed in LRRC59 LUAD network and closely associated with lymph node metastasis (P <0.001), TNM stage (P <0.001), and histological differentiation (P = 0.007). Further multivariate analysis showed that excessive LRRC59 an independent prognostic factor in LUAD.


Conclusion: LRRC59 can serve as novel biomarkers and therapeutic targets for clinical practice LUAD.

 Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma
Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma

LncRNA MIR4435-2HG inhibit the progression of osteoarthritis through sponges mir-510-3p

Osteoarthritis (OA) is a joint disorder diarthrodial which can have several causes. Long non-coding RNA (lncRNAs) participated in several diseases, including OA. Recently reported that microRNA lncRNA 4435-2HG (MIR4435-2HG) is downregulated in OA tissue; However, the biological role during the progression of OA MIR4435-2HG still unclear. In this study, interleukin (IL) -1β used to build an in vitro model of OA. protein expression of matrix metallopeptidase (MMP) 1, MMP13, collagen II, interleukin (IL) -17A, p65, phosphorylated (p) -p65, IκB and p-IκB in CHON-001 cells were detected by western blotting.

Gene expression of IL-17A, and miR-510-3p MIR4435-2HG network or CHON-001 cells was measured by reverse transcription-quantitative PCR and western blotting, respectively. Cells Counting Kit-8 assay and immunofluorescence staining were used to investigate cell proliferation, and cell apoptosis was detected by flow cytometry. The relationship between MIR4435-2HG, miR-510-3p and IL-17A was investigated using a dual luciferase assay report. MIR4435-2HG and excess miR-510-3p CHON-transfected into 001 cells.

The results show that excess miR4435-2HG significantly increase proliferation and inhibit apoptosis CHON-001 cells. In addition, miR-510-3p identified as a downstream target MIR4435-2HG, and miR-510-3p directly targeted IL-17A. The results of this study indicate that MIR4435-2HG can mediate the development of OA by disabling NF-kB signaling pathway.

Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
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SafePinky DNA Gel Staining Solution

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SafePinky DNA Gel Staining Solution

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SafePinky DNA Gel Staining Solution

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Gelite™ Green Nucleic Acid Gel Staining Kit

17589 1 Kit
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Gelite™ Orange Nucleic Acid Gel Staining Kit

17594 1 Kit
EUR 219

Prosi Silver Staining Kit for 25 mini gel

S3200-020 1kit
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DAB Enhancer for ampliying DAB stains in IHC staining, 60ml

NB308 60 ml
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DAB Enhancer for ampliying DAB stains in IHC staining, 30ml

NB308-30 30 ml
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SafePinky DNA Gel Staining Solution(10,000X) In Water

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SafePinky DNA Gel Staining Solution(10,000X) In Water

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SafePinky DNA Gel Staining Solution(10,000X) In Water

S1001-100 1ml
EUR 435

SafePinky DNA Gel Staining Solution(10,000X) In Water

S1001-500 5x1ml
EUR 1643

Safe E-Red DNA Gel Staining Solution(1000x)

S1002-001 1ml
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Safe E-Red DNA Gel Staining Solution(1000x)

S1002-005 5x1ml
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Safe E-Red DNA Gel Staining Solution(1000x)

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EUR 300

Safe E-Red DNA Gel Staining Solution(1000x)

S1002-020 20ml
EUR 496

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P141-ALL - Ask for price

Leukemia - ALL Exosome RNA

P241-ALL - Ask for price

?-Galactosidase Staining Kit

AKR-100 1 kit
EUR 432
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 230

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 230

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012 10 ml
EUR 185

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012-L 10x10ml
EUR 1587

Gel-Bright LED Gel Illuminator

E90003 1EA
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Description: Minimum order quantity: 1 unit of 1EA

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 379

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 370
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Beta-Galactosidase Staining Kit

K802-250
EUR 294

Human Histochemistry Staining Kit

20-abx299039
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Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 230

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 325.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 293

Quick Gel Extraction Kit

20-abx098085
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  • 200 rxns
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Gel DNA Recovery Kit

GP05 5 preps Ask for price

Gel DNA Recovery Kit

GP100 100 preps
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GP200 200 preps
EUR 189

Gel DNA Recovery Kit

GP50 50 preps
EUR 94

AmbiClean Kit (PCR & Gel)

GV05 5 preps Ask for price

AmbiClean Kit (PCR & Gel)

GV100 100 preps
EUR 128

AmbiClean Kit (PCR & Gel)

GV200 200 preps
EUR 189

AmbiClean Kit (PCR & Gel)

GV50 50 preps
EUR 91

Gel Cutter

KS071012-11 12
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Gel Filter

KS071012-13 12
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Gel Tray

2394250 4unit
EUR 270
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Gel Tray

2398194 2unit
EUR 354

TC Gel

CP048-005 500 g
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TC Gel

CP048-010 1 Kg
EUR 347

Frit Kit

FRIT-KIT 1each
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CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 638
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Live-Dead Cell Staining Kit

K501-100
EUR 468

DNA Ploidy Analysis Staining Kit

K1439-1
EUR 588

DNA Ploidy Analysis Staining Kit

K1439-5
EUR 2002

Nerve Terminal Staining Kit V

70034 1SET
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Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 262

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AP100D-1 100 Assays
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AP100R-1 50 Assays
EUR 262

Fluorescein Active Caspase Staining Kit

K2047-100 100 assays
EUR 572

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 238

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K2052-100 100 assays
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K2052-25 25 assays
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Live-Dead Cell Staining Kit

K2081-100 100 staining
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Column Packing Kit

PACK-KIT 1pack
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PCR Mycoplasma Detection Kit

M034-Kit Kit
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Gel Extraction Kit (100 preps)

9K-006-0001 100preps
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9K-006-0002 200preps, 200prep
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SDS-PAGE Gel Preparation Kit

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SDS-PAGE Gel Preparation Kit

abx090646-1Kit 1 Kit
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ISOLATE II PCR & Gel Kit

BIO-52058 10 preps Ask for price

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BIO-52060 250 preps Ask for price

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P-1007 100 Samples
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GEL Purification Mini Kit (50prep)

FAGPK-001 50 preps
EUR 120

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FAGPK-001-1 200 preps
EUR 169

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FAGPK-001-2 300 preps
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FAMGK-001 50 preps
EUR 126

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FAMGK-001-1 200 preps
EUR 187

Zenoquick Gel DNA Recovery Kit

Z3001-050 50 preps
EUR 199

Zenoquick Gel DNA Recovery Kit

Z3001-200 4x50 preps
EUR 631

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
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2322112 5unit
EUR 257
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2322117 5unit
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Aluminium Phosphate Gel

VAdv-Ly0001 250 mL
EUR 1386
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VAdv-Ly0003 250 mL
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ViSafe RED Gel

S430 500 µl
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S430L 5x500 µl
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S435 500 µl
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Ketanserin (Vulketan Gel)

E1KS2232 50mg
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Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
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CaspGLOW Active Caspase 12 Staining Kit

55R-1291 25 assays
EUR 282
Description: Caspase 12 Staining Kit for use activity in the research laboratory

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55R-1293 25 assays
EUR 284
Description: Caspase 2 Staining Kit for use activity in the research laboratory

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55R-1294 25 assays
EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory

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55R-1295 25 assays
EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory

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55R-1296 25 assays
EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

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55R-1297 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

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K580-24
EUR 452

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-100
EUR 550

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K180-25
EUR 234

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K190-100
EUR 523

CaspGLOW? Red Active Caspase Staining Kit

K190-25
EUR 240

Human Type I Collagen Staining Kit

9044 1 kit
EUR 338.55
Description: Human Type I Collagen Staining Kit

Yeast Viability Staining Kit (Green/Red)

31063-1 1kit
EUR 472
Description: Minimum order quantity: 1 unit of 1kit

StemTAG Alkaline Phosphatase Staining Kit (Red)

CBA-300 100 assays
EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

StemTAG Alkaline Phosphatase Staining Kit (Purple)

CBA-306 100 assays
EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 244

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 208

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 128

Fluorescein Active Caspase-12 Staining Kit

K2046-100 100 assays
EUR 572

Fluorescein Active Caspase-12 Staining Kit

K2046-25 25 assays
EUR 238

Fluorescein Active Caspase-2 Staining Kit

K2048-100 100 assays
EUR 572

Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
EUR 238

Fluorescein Active Caspase-3 Staining Kit

K2049-100 100 assays
EUR 572

Fluorescein Active Caspase-3 Staining Kit

K2049-25 25 assays
EUR 238

Fluorescein Active Caspase-8 Staining Kit

K2050-100 100 assays
EUR 572

Fluorescein Active Caspase-8 Staining Kit

K2050-25 25 assays
EUR 238

Fluorescein Active Caspase-9 Staining Kit

K2051-100 100 assays
EUR 572

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 238

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 572

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 238

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 572

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 238

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 572

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 238

Human FoxP3 Transcription Factor Staining Kit

OKTA00001 KIT
EUR 454
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Human FoxP3 Transcription Factor Staining Kit

OKTA00004 KIT
EUR 454
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132

PI / RNase Staining Buffer

20-abx090619
  • EUR 272.00
  • EUR 230.00
  • 100 tests
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Ponceau S Staining Solution

PW005 100ml
EUR 69.14

Ponceau S Staining Solution

B7778-100000 100 g
EUR 163

Ponceau S Staining Solution

B7778-50000 50 g
EUR 126

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 176

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1132

Cas9 SmartNuclease Extra Ligation Kit [includes 5x ligation buffer (10 ul) and Fast ligase (2.5ul)]

CAS9LIG-KIT 1 Kit
EUR 153

PinPoint-FC 293T Platform Kit for Targeted Gene Insertion (includes PIN320A-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN320A-KIT 1 Kit
EUR 4941

Gel and PCR DNA Purification kit

K1455-250 250 Preps
EUR 480

Gel and PCR DNA Purification kit

K1455-50 50 Preps
EUR 206

FastPure Gel DNA Extraction Mini Kit

DC301-01 100 rxn
EUR 129

LumiPure DNA Gel Extraction Kit, 50

23793 50 preps
EUR 75

DNA Gel Extraction Kit - 250 Columns

31030-250 1kit
EUR 545
Description: Minimum order quantity: 1 unit of 1kit

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31030-50 1kit
EUR 155
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Protein Extraction Kit from Gel Slices

P519-20 - Ask for price

Protein Extraction Kit from Gel Slices

P519-80 - Ask for price

MicroElute GEL/PCR Purification Kit (50prep)

FAEPK-001 50 preps
EUR 131

MicroElute GEL/PCR Purification Kit (200prep)

FAEPK-001-1 200 preps
EUR 208

MicroElute GEL/PCR Purification Kit (300prep)

FAEPK-001-2 300 preps
EUR 229

GEL/PCR Purification Mini Kit (100prep)

FAGCK-001 100 preps
EUR 129

GEL/PCR Purification Mini Kit (300prep)

FAGCK-001-1 300 preps
EUR 172

PinPoint-FC Murine iPSC Platform Kit for Targeted Gene Insertion (includes PIN340iPS-1, PIN200A-1, PIN510A-1 & PIN600A-1)

PIN340iPS-KIT 1 Kit
EUR 4941

CaspGLOW Red Active Caspase 3 Staining Kit

55R-1298 25 assays
EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 8 Staining Kit

55R-1299 25 assays
EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 9 Staining Kit

55R-1300 25 assays
EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

EZCell? Intracellular Zinc Staining Kit (Cell-Based)

K525-50
EUR 566

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-100
EUR 523

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-25
EUR 234

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-100
EUR 550

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-25
EUR 234

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

K449-50
EUR 544

Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based)

K458-50
EUR 572

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-100
EUR 533

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-25
EUR 240

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-100
EUR 550

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-25
EUR 240

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-100
EUR 550

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-25
EUR 240

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-100
EUR 523

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-25
EUR 240

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-100
EUR 517

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-25
EUR 240

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-100
EUR 523

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-25
EUR 234

Type IX Collagen Staining Kit, Multi-Species

9012 1 kit
EUR 338.55
Description: Type IX Collagen Staining Kit

Sirius Red/Fast Green Collagen Staining Kit

9046 30-50 samples
EUR 137
Description: Sirius Red/Fast Green Collagen Staining Kit

In addition, excess miR4435-2HG inhibit the development of OA, indicating that miR4435-2HG can be considered as a potential therapeutic target in OA.

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