Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma

Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma

Objective: LRRC59 (leucine-rich repeat-containing protein 59) is a ribosome-binding protein which also interacts with the fibroblast growth factor. limited investigation reveals the possibility of LRRC59 role in aggressive phenotype of breast cancer. However, if LRRC59 contribute to the development of lung cancer is still unclear.


Materials and Methods: In this study, a software-based survival analysis TCGA online (GEPIA2) was used to estimate the prognostic value LRRC59 mRNA expression levels for lung cancer. Cells Counting Kit-8 assay, colony-forming assay, cell cycle analysis, and transwell test used to assess the biological function LRRC59 in lung cancer cells. Then, 94 lung adenocarcinoma (LUAD) patient tissue were collected to test the level LRRC59 expression by tissue microarray (TMA) based immunohistochemical staining (IHC). Kaplan-Meier univariate and multivariate Cox regression analysis was performed to evaluate the prognostic value of protein expression in LUAD LRRC59.


Results: High Level of LRRC59 mRNA was significantly associated with poorer survival for lung adenocarcinoma, but not for lung squamous cell carcinoma. ShRNA knockdown of LRRC59 by apparently inhibiting cell proliferation and colony formation in both H1299 and A549 cells. G1 / S phase arrest caused by depletion LRRC59 observed in A549 and H1299 cells. In addition, silencing LRRC59 decreased migrative cells and invasive ability. In addition, the TMA-based IHC showed that it was highly expressed in LRRC59 LUAD network and closely associated with lymph node metastasis (P <0.001), TNM stage (P <0.001), and histological differentiation (P = 0.007). Further multivariate analysis showed that excessive LRRC59 an independent prognostic factor in LUAD.


Conclusion: LRRC59 can serve as novel biomarkers and therapeutic targets for clinical practice LUAD.

 Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma
Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma

LncRNA MIR4435-2HG inhibit the progression of osteoarthritis through sponges mir-510-3p

Osteoarthritis (OA) is a joint disorder diarthrodial which can have several causes. Long non-coding RNA (lncRNAs) participated in several diseases, including OA. Recently reported that microRNA lncRNA 4435-2HG (MIR4435-2HG) is downregulated in OA tissue; However, the biological role during the progression of OA MIR4435-2HG still unclear. In this study, interleukin (IL) -1β used to build an in vitro model of OA. protein expression of matrix metallopeptidase (MMP) 1, MMP13, collagen II, interleukin (IL) -17A, p65, phosphorylated (p) -p65, IκB and p-IκB in CHON-001 cells were detected by western blotting.

Gene expression of IL-17A, and miR-510-3p MIR4435-2HG network or CHON-001 cells was measured by reverse transcription-quantitative PCR and western blotting, respectively. Cells Counting Kit-8 assay and immunofluorescence staining were used to investigate cell proliferation, and cell apoptosis was detected by flow cytometry. The relationship between MIR4435-2HG, miR-510-3p and IL-17A was investigated using a dual luciferase assay report. MIR4435-2HG and excess miR-510-3p CHON-transfected into 001 cells.

The results show that excess miR4435-2HG significantly increase proliferation and inhibit apoptosis CHON-001 cells. In addition, miR-510-3p identified as a downstream target MIR4435-2HG, and miR-510-3p directly targeted IL-17A. The results of this study indicate that MIR4435-2HG can mediate the development of OA by disabling NF-kB signaling pathway.

Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
EUR 226.8

Coomassie Brilliant Blue Gel Staining Kit

abx090653-100l 100 µl
EUR 125

Coomassie Brilliant Blue Gel Staining Kit

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Coomassie Brilliant Blue Gel Staining Kit

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ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

Geliteâ„¢ Green Nucleic Acid Gel Staining Kit

17589 1 Kit
EUR 227
Description: Gelite™ Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels.

Geliteâ„¢ Green Nucleic Acid Gel Staining Kit

17589-1Kit 1 Kit
EUR 222
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Geliteâ„¢ Orange Nucleic Acid Gel Staining Kit

17594 1 Kit
EUR 227
Description: Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film.

Geliteâ„¢ Orange Nucleic Acid Gel Staining Kit

17594-1Kit 1 Kit
EUR 222
Description: Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film.

EnoGeneEXBlueTM One Step Rapid Gel Staining kit

EGU002-100 100ml
EUR 60

EnoGeneEXBlueTM One Step Rapid Gel Staining kit

EGU002-500 500ml
EUR 90

Prosi Silver Staining Kit for 25 mini gel

S3200-020 1kit
EUR 433.2

SafePinky DNA Gel Staining Solution

S1000-050 500ml
EUR 157.2

SafePinky DNA Gel Staining Solution

S1000-100 2X500ml
EUR 225.6

SafePinky DNA Gel Staining Solution

S1000-200 4X500ml
EUR 336

Phosphorylated protein gel fluorescent staining kit

E1WP329 250mL / 5 gels
EUR 300

dsGreen Gel Staining Solution, 10000×, 50 uL

A0010 50 uL
EUR 62.4

Safe E-Red DNA Gel Staining Solution(1000x)

S1002-001 1ml
EUR 133.2

Safe E-Red DNA Gel Staining Solution(1000x)

S1002-005 5x1ml
EUR 271.2

Safe E-Red DNA Gel Staining Solution(1000x)

S1002-010 10ml
EUR 360

Safe E-Red DNA Gel Staining Solution(1000x)

S1002-020 20ml
EUR 595.2

ProLiteâ„¢ His-Tag Protein Gel Staining Kit *Green Fluorescence*

18010 10 Gels
EUR 421
Description: The polyhistidine tag (His-Tag) is widely used for protein purification, detection, and immobilization.

ProLiteâ„¢ His-Tag Protein Gel Staining Kit *Green Fluorescence*

18010-10Gels 10 Gels
EUR 412
Description: The polyhistidine tag (His-Tag) is widely used for protein purification, detection, and immobilization.

SafePinky DNA Gel Staining Solution(10,000X) In Water

S1001-025 250ul
EUR 193.2

SafePinky DNA Gel Staining Solution(10,000X) In Water

S1001-050 2x250ul
EUR 296.4

SafePinky DNA Gel Staining Solution(10,000X) In Water

S1001-100 1ml
EUR 522

SafePinky DNA Gel Staining Solution(10,000X) In Water

S1001-500 5x1ml
EUR 1971.6

SafeGlow Green DNA Gel Staining Solution(10,000X) In Water

S1002-025 250ul
EUR 82

SafeGlow Green DNA Gel Staining Solution(10,000X) In Water

S1002-050 2x250ul
EUR 123

SafeGlow Green DNA Gel Staining Solution(10,000X) In Water

S1002-100 1ml
EUR 205

SafeGlow Green DNA Gel Staining Solution(10,000X) In Water

S1002-500 5x1ml
EUR 990

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 276

HE Staining Kit

E-IR-R117-100mL 100mL
EUR 240

HE Staining Kit

E-IR-R117-10mL 10mL
EUR 35

HE Staining Kit

E-IR-R117-50mL 50mL
EUR 150

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DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

TRAP Staining Kit

KT-008 1 kit
EUR 747

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abx097206-100l 100 µl
EUR 162.5

DAPI Staining Kit

abx097206-1ml 1 ml Ask for price

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Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 276

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EUR 162.5

Hoechst 33342 Staining Kit

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Cell Cycle Staining Kit

KTA2020-100T 100 T
EUR 119
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-50T 50 T
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Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit

KTA2020-each each Ask for price

Live NucRed staining kit

EGY069 >100T
EUR 210

SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)

BMU105-EN-250mL2 250 mL×2
EUR 59
Description: Protein Gel Staining

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EGY068 >100T
EUR 210

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EGY070 >100T
EUR 210

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 494.4

STAT Double Staining ragent for Double staining Mo

NB330DB 25 ml
EUR 595
Description: STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides

STAT Double Staining reagent for Double staining o

NB326DB 25 ml
EUR 595
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STAT Double Staining Reagent for Double staining o

NB327DB 25 ml
EUR 595
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Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 500
Description: Live/Dead Cell Staining Kit for use in the research laboratory

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AP100R-1 50 Assays
EUR 314.4

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AP100B-1 50 Assays
EUR 203

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 390.96

β-Galactosidase Staining Kit

AKR-100 1 kit
EUR 345

Live-Dead Cell Staining Kit

GWB-AXR262 100 stainings Ask for price

Live-Dead Cell Staining Kit

K501-100 each
EUR 561.6

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 552

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K2081-1000 1000T
EUR 112

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EUR 68

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 300
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Nerve Terminal Staining Kit I

70030-1 KT
EUR 605

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70034 1SET
EUR 605
Description: N/A

Nerve Terminal Staining Kit V

70034-1 KT
EUR 605

Purple-Color AP Staining Kit

AP100P-1 100 Assays
EUR 235

Lipid (Oil Red O) staining Kit

K580-24 each
EUR 542.4

Stains-all

03943-1 1g
EUR 71
Description: 7423-31-6

Stains-all

03943-5 5g
EUR 244
Description: 7423-31-6

Stains-All

D6359 1g
EUR 126.82

Stains-all

581275 1.0g
EUR 230

Stains-All

GL8815-1 1
EUR 63.3

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GL8815-5 5
EUR 221.5

Stains-All

T16939-10mg 10mg Ask for price
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Stains-All

HY-D0987 200mg
EUR 129.6

Human Histochemistry Staining Kit

20-abx299039
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  • 500 tests
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Beta-Galactosidase Staining Kit

GWB-AXR372 200 assays. Ask for price

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 274

Beta-Galactosidase Staining Kit

K802-250 each
EUR 352.8

Nerve Terminal Staining Kit IIA

70031-1 1SET
EUR 322
Description: N/A

Nerve Terminal Staining Kit IIB

70031-1-1 KT
EUR 395

Nerve Terminal Staining Kit III

70032-1 KT
EUR 513

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 418
Description: Detects activated Caspases by FACS, FL or plate reader

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 285.6

DAPI staining kit (ready to use) 

EGY0621 10mL
EUR 85

DAPI staining kit (ready to use) 

EGY0622 50mL
EUR 185

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 127.28

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 63

DNA Ploidy Analysis Staining Kit

K1439-1 each
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5 each
EUR 2402.4

Propidium Iodide (PI) Staining Kit

abx097208-100l 100 µl
EUR 162.5

Propidium Iodide (PI) Staining Kit

abx097208-1ml 1 ml Ask for price

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Stayrightâ„¢ Purple HRP Staining Kit

45905 5 mL
EUR 112
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45905-5mL 5 mL
EUR 109
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45906 50 mL
EUR 341
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45906-50mL 50 mL
EUR 334
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Hematoxylin and Eosin Staining Kit

K1142-1000 2×500mL
EUR 62
Description: Hematoxylin and Eosin Staining,one of the most widely used staining technique in histopathology

TRAP Staining Kit with Osteoplates

KT-651 1 kit
EUR 1003

Hochst33342/PI Double Staining Kit

E37F032-100-tests 100tests
EUR 66.67
Description: Double staining kit

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 292.8

Calcein AM/PI Double Staining Kit

E-CK-A354-each each Ask for price
Description: Viability/Cytotoxicity

STAT-Q Goat AEC KIT for staining all Goat primary

NB314KLGC-20 20 ml kit
EUR 589
Description: STAT-Q Goat AEC KIT for staining all Goat primary antibodies, 350 plus slides

STAT-Q Goat DAB KIT for staining all Goat primary

NB314KLGD-20 20 ml kit
EUR 589
Description: STAT-Q Goat DAB KIT for staining all Goat primary antibodies, 350 plus slides

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 613
Description: Caspase Staining Kit for use activity in the research laboratory

Hoechst 33342 staining kit (ready to use) 

EGY0631 10mL
EUR 50

Hoechst 33342 staining kit (ready to use) 

EGY0632 50mL
EUR 160

Hoechst 33258 staining kit (ready to use) 

EGY0641 10mL
EUR 50

Hoechst 33258 staining kit (ready to use) 

EGY0642 50mL
EUR 145

Protein Stain-EZ J Phosphoprotein Staining Kit

BSP043 2x10minigel, 20prep
EUR 133.08

STAT-Q Rabbit AEC KIT for staining all Rabbit anti

NB314KLRC-20 20 ml kit
EUR 589
Description: STAT-Q Rabbit AEC KIT for staining all Rabbit antibodies, 350 plus slides

STAT-Q Rabbit DAB KIT for staining all Rabbit anti

NB314KLRD-20 20 ml kit
EUR 585
Description: STAT-Q Rabbit DAB KIT for staining all Rabbit antibodies, 350 plus slides

Calcein-AM/PI Double Staining Kit

E-CK-A354-100Assays 100 Assays
EUR 100

Calcein-AM/PI Double Staining Kit

E-CK-A354-2000Assays 2000 Assays
EUR 600

Calcein-AM/PI Double Staining Kit

E-CK-A354-500Assays 500 Assays
EUR 300

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 610
Description: Detects activated Caspases-3 by FACS, FL or plate reader

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 285.6

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 490
Description: Detects activated Caspases-8 by FACS, FL or plate reader

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 285.6

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 610
Description: Detects activated Caspases-9 by FACS, FL or plate reader

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 285.6

Calcein-AM/PI Double Staining Kit

E37F030-500-tests 500tests
EUR 266.67
Description: Double staining kit

Calcein-AM/PI Double Staining Kit

E37F031-1000-tests 1000tests
EUR 526.67
Description: Double staining kit

Alkaline Phosphatase Red Staining kit

TBS2080 50 tests
EUR 89

Four color suit nuclear staining Kit

EGY071 100T
EUR 1190

Alkaline Phosphatase Blue Staining kit

TBS2085 50 tests
EUR 89

Yeast Viability Staining Kit (Red/Blue)

31063-2-1 KT
EUR 475

Foxp3/Transcription Factor Staining Kit

E-CK-A108-20Assays 20 Assays
EUR 75

Fluorescein Active Caspase Staining Kit

K2047-100 100 assays
EUR 610
Description: Detects activated Caspases by FACS, FL or plate reader

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 285.6

Foxp3/Transcription Factor Staining Kit

E-CK-A108-each each Ask for price

Yeast Viability Staining Kit (Green/Red)

31063-1 1kit
EUR 475
Description: N/A

Yeast Viability Staining Kit (Green/Red)

31063-1-1 KT
EUR 475

Coplin Staining Jar

08415-3 3sets
EUR 84

Live and Dead Cell Double Staining Kit

KTA1001-100T 100 T
EUR 79
Description: Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells.

Live and Dead Cell Double Staining Kit

KTA1001-2000T 2000 T
EUR 239
Description: Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells.

Live and Dead Cell Double Staining Kit

KTA1001-500T 500 T
EUR 139
Description: Abbkine Live and Dead Cell Double Staining Kit provides a convenient assay to evaluate the viability of cells.

CaspGLOW Active Caspase 12 Staining Kit

55R-1291 25 assays
EUR 338.4
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit

55R-1293 25 assays
EUR 580
Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit

55R-1294 25 assays
EUR 613
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit

55R-1295 25 assays
EUR 613
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 9 Staining Kit

55R-1296 25 assays
EUR 613
Description: Caspase 9 Staining Kit for use activity in the research laboratory

Senescence β-Galactosidase Staining Kit

KTA3030-100T 100 T
EUR 119
Description: Senescence β-galactosidase staining kit provides a simple and convenient operation method for staining detection of senescent cells or tissues; This kit only stains senescent cells, and does not stain pre-senescence cells (senescent cells), quiescent cells (static cells), immortal cells (immortal cells) or tumor cells.

Senescence β-Galactosidase Staining Kit

KTA3030-each each Ask for price

Senescence β-Galactosidase Staining Kit

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Cell Staining Buffer

E-CK-A107-100mL 100 mL
EUR 20

Cell Staining Buffer

E-CK-A107-200mL 200 mL
EUR 35

Cell Staining Buffer

E-CK-A107-500mL 500 mL
EUR 60

Cell Staining Buffer

E16FXP005 500 ml
EUR 40
Description: Available in various conjugation types.

DNA Staining Reagent

abx299713-100g 100 µg Ask for price

DNA Staining Reagent

abx299713-20g 20 µg
EUR 300

DNA Staining Reagent

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Cell Staining Buffer

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Nuclear 7-AAD fluorescent staining kit

EGY066 >100T
EUR 195

CaspGLOW Red Active Caspase Staining Kit

55R-1297 25 assays
EUR 580
Description: Caspase Staining Kit for use activity in the research laboratory

CaspGLOW? Red Active Caspase Staining Kit

K190-100 each
EUR 627.6

CaspGLOW? Red Active Caspase Staining Kit

K190-25 each
EUR 288

Trypan Blue Staining Cell Assay Kit

APB0003-10 5ml
EUR 0.1

Fluorescein Active Caspase-2 Staining Kit

K2048-100 100 assays
EUR 610
Description: Detects activated Caspase-2 by FACS, FL or plate reader

Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
EUR 285.6

Fluorescein Active Caspase-3 Staining Kit

K2049-100 100 assays
EUR 508
Description: Detects activated Caspase-3 by FACS, FL or plate reader

Fluorescein Active Caspase-3 Staining Kit

K2049-25 25 assays
EUR 285.6

Fluorescein Active Caspase-8 Staining Kit

K2050-100 100 assays
EUR 610
Description: Detects activated Caspase-8 by FACS, FL or plate reader

Fluorescein Active Caspase-8 Staining Kit

K2050-25 25 assays
EUR 285.6

Fluorescein Active Caspase-9 Staining Kit

K2051-100 100 assays
EUR 442
Description: Detects activated Caspase-9 by FACS, FL or plate reader

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 285.6

Yeast Viability Staining Kit (Far-Red/Red)

31063-3-1 KT
EUR 475

Propidium iodide staining kit (ready to use) 

EGY0651 5mL
EUR 90

Propidium iodide staining kit (ready to use) 

EGY0652 10mL
EUR 125

Propidium iodide staining kit (ready to use) 

EGY0653 20mL
EUR 240

DNA STAINING AGENT (1ML)

NOVELJUICE each
EUR 80

Fluorescein Active Caspase-12 Staining Kit

K2046-100 100 assays
EUR 571
Description: Detects activated Caspase-12 by FACS, FL or plate reader

Fluorescein Active Caspase-12 Staining Kit

K2046-25 25 assays
EUR 285.6

In addition, excess miR4435-2HG inhibit the development of OA, indicating that miR4435-2HG can be considered as a potential therapeutic target in OA.

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