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Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma

Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma

Objective: LRRC59 (leucine-rich repeat-containing protein 59) is a ribosome-binding protein which also interacts with the fibroblast growth factor. limited investigation reveals the possibility of LRRC59 role in aggressive phenotype of breast cancer. However, if LRRC59 contribute to the development of lung cancer is still unclear.


Materials and Methods: In this study, a software-based survival analysis TCGA online (GEPIA2) was used to estimate the prognostic value LRRC59 mRNA expression levels for lung cancer. Cells Counting Kit-8 assay, colony-forming assay, cell cycle analysis, and transwell test used to assess the biological function LRRC59 in lung cancer cells. Then, 94 lung adenocarcinoma (LUAD) patient tissue were collected to test the level LRRC59 expression by tissue microarray (TMA) based immunohistochemical staining (IHC). Kaplan-Meier univariate and multivariate Cox regression analysis was performed to evaluate the prognostic value of protein expression in LUAD LRRC59.


Results: High Level of LRRC59 mRNA was significantly associated with poorer survival for lung adenocarcinoma, but not for lung squamous cell carcinoma. ShRNA knockdown of LRRC59 by apparently inhibiting cell proliferation and colony formation in both H1299 and A549 cells. G1 / S phase arrest caused by depletion LRRC59 observed in A549 and H1299 cells. In addition, silencing LRRC59 decreased migrative cells and invasive ability. In addition, the TMA-based IHC showed that it was highly expressed in LRRC59 LUAD network and closely associated with lymph node metastasis (P <0.001), TNM stage (P <0.001), and histological differentiation (P = 0.007). Further multivariate analysis showed that excessive LRRC59 an independent prognostic factor in LUAD.


Conclusion: LRRC59 can serve as novel biomarkers and therapeutic targets for clinical practice LUAD.

Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma
Overexpression of LRRC59 Is Associated with Poor Prognosis and Promotes Cell Proliferation and Invasion in Lung Adenocarcinoma

LncRNA MIR4435-2HG inhibit the progression of osteoarthritis through sponges mir-510-3p

Osteoarthritis (OA) is a joint disorder diarthrodial which can have several causes. Long non-coding RNA (lncRNAs) participated in several diseases, including OA. Recently reported that microRNA lncRNA 4435-2HG (MIR4435-2HG) is downregulated in OA tissue; However, the biological role during the progression of OA MIR4435-2HG still unclear. In this study, interleukin (IL) -1β used to build an in vitro model of OA. protein expression of matrix metallopeptidase (MMP) 1, MMP13, collagen II, interleukin (IL) -17A, p65, phosphorylated (p) -p65, IκB and p-IκB in CHON-001 cells were detected by western blotting.

Gene expression of IL-17A, and miR-510-3p MIR4435-2HG network or CHON-001 cells was measured by reverse transcription-quantitative PCR and western blotting, respectively. Cells Counting Kit-8 assay and immunofluorescence staining were used to investigate cell proliferation, and cell apoptosis was detected by flow cytometry. The relationship between MIR4435-2HG, miR-510-3p and IL-17A was investigated using a dual luciferase assay report. MIR4435-2HG and excess miR-510-3p CHON-transfected into 001 cells.

The results show that excess miR4435-2HG significantly increase proliferation and inhibit apoptosis CHON-001 cells. In addition, miR-510-3p identified as a downstream target MIR4435-2HG, and miR-510-3p directly targeted IL-17A. The results of this study indicate that MIR4435-2HG can mediate the development of OA by disabling NF-kB signaling pathway.

Coomassie Brilliant Blue Gel Staining Kit
abx090653-100l 100 µl
EUR 125

Coomassie Brilliant Blue Gel Staining Kit
abx090653-1Kit 1 Kit
EUR 226.8

Coomassie Brilliant Blue Gel Staining Kit
abx090653-1ml 1 ml Ask for price

Coomassie Brilliant Blue Gel Staining Kit
abx090653-200l 200 µl Ask for price

Coomassie Brilliant Blue Gel Staining Kit
MBS355543-100mL 100mL
EUR 170

Coomassie Brilliant Blue Gel Staining Kit
MBS355543-5x100mL 5x100mL
EUR 460

ProsiBlue Gel Staining Solution
P7300-050 500ml
EUR 102

Geliteâ„¢ Green Nucleic Acid Gel Staining Kit
17589 1 Kit
EUR 227
Description: Gelite™ Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels.

Geliteâ„¢ Green Nucleic Acid Gel Staining Kit
17589-1Kit 1 Kit
EUR 222
Description: Gelite™ Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels.

Geliteâ„¢ Orange Nucleic Acid Gel Staining Kit
17594 1 Kit
EUR 227
Description: Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film.

Geliteâ„¢ Orange Nucleic Acid Gel Staining Kit
17594-1Kit 1 Kit
EUR 222
Description: Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film.

EnoGeneEXBlueTM One Step Rapid Gel Staining kit
EGU002-100 100ml
EUR 60

EnoGeneEXBlueTM One Step Rapid Gel Staining kit
EGU002-500 500ml
EUR 90

SafePinky DNA Gel Staining Solution
S1000-050 500ml
EUR 68

SafePinky DNA Gel Staining Solution
S1000-100 2X500ml
EUR 110

SafePinky DNA Gel Staining Solution
S1000-200 4X500ml
EUR 180

Prosi Silver Staining Kit for 25 mini gel
S3200-020 1kit
EUR 433.2

Phosphorylated protein gel fluorescent staining kit
E1WP329 250mL / 5 gels
EUR 300

Safe E-Red DNA Gel Staining Solution(1000x)
S1002-001 1ml
EUR 133.2

Safe E-Red DNA Gel Staining Solution(1000x)
S1002-005 5x1ml
EUR 271.2

Safe E-Red DNA Gel Staining Solution(1000x)
S1002-010 10ml
EUR 360

Safe E-Red DNA Gel Staining Solution(1000x)
S1002-020 20ml
EUR 595.2

ProLiteâ„¢ His-Tag Protein Gel Staining Kit *Green Fluorescence*
18010 10 Gels
EUR 421
Description: The polyhistidine tag (His-Tag) is widely used for protein purification, detection, and immobilization.

ProLiteâ„¢ His-Tag Protein Gel Staining Kit *Green Fluorescence*
18010-10Gels 10 Gels
EUR 412
Description: The polyhistidine tag (His-Tag) is widely used for protein purification, detection, and immobilization.

SuperKine Protein Gel Fast Staining Solution
MBS9719198-2x250mL 2x250mL
EUR 115

SafePinky DNA Gel Staining Solution(10,000X) In Water
S1001-025 250ul
EUR 85

SafePinky DNA Gel Staining Solution(10,000X) In Water
S1001-050 2x250ul
EUR 128

SafePinky DNA Gel Staining Solution(10,000X) In Water
S1001-100 1ml
EUR 220

SafePinky DNA Gel Staining Solution(10,000X) In Water
S1001-500 5x1ml
EUR 990

DAB Enhancer for ampliying and darkening DAB stains in IHC staining, 60 ml (Large volume)
NB308 60 ml
EUR 435

DAB Enhancer for ampliying and darkening DAB stains in IHC staining, 30 ml (Small volume)
NB308-30 30 ml
EUR 285

SafeGlow Green DNA Gel Staining Solution(10,000X) In Water
S1002-025 250ul
EUR 82

SafeGlow Green DNA Gel Staining Solution(10,000X) In Water
S1002-050 2x250ul
EUR 123

SafeGlow Green DNA Gel Staining Solution(10,000X) In Water
S1002-100 1ml
EUR 205

SafeGlow Green DNA Gel Staining Solution(10,000X) In Water
S1002-500 5x1ml
EUR 990

HE Staining Kit
E-IR-R117-100mL 100mL
EUR 240

HE Staining Kit
E-IR-R117-10mL 10mL
EUR 35

HE Staining Kit
E-IR-R117-50mL 50mL
EUR 150

HE Staining Kit
E-IR-R117-each each Ask for price

PI Staining Kit
abx097208-1Kit 1 Kit
EUR 276

HE staining Kit
MBS2567140-100mL 100mL
EUR 255

HE staining Kit
MBS2567140-10mL 10mL
EUR 120

HE staining Kit
MBS2567140-50mL 50mL
EUR 195

HE staining Kit
MBS2567140-5x100mL 5x100mL
EUR 1120

PI Staining Kit
MBS826722-01mL 0.1mL
EUR 140

PI Staining Kit
MBS826722-5x01mL 5x0.1mL
EUR 550

DAPI Staining Kit
abx097206-100l 100 µl
EUR 162.5

DAPI Staining Kit
abx097206-1Kit 1 Kit
EUR 276

DAPI Staining Kit
abx097206-1ml 1 ml Ask for price

DAPI Staining Kit
abx097206-200l 200 µl Ask for price

TRAP Staining Kit
KT-008 1 kit
EUR 747

DAPI Staining Kit
MBS826728-01mL 0.1mL
EUR 140

DAPI Staining Kit
MBS826728-5x01mL 5x0.1mL
EUR 550

Silver Staining Kit
ML123-1KT 1 unit
EUR 201.16
Description: Silver Staining Kit

SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)
BMU105-EN-250mL2 250 mL×2
EUR 59
Description: Protein Gel Staining

SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)
BMU105-EN-each each Ask for price

SuperKine™ Protein Gel Fast Staining Solution (Coomassie Blue)
BMU105-EN-Null Null Ask for price

ELVIS ID Staining Kit
SK-ELVIS-100 100 Tests Ask for price

ELVIS ID Staining Kit
SK-ELVIS-1000 1000 Tests Ask for price

ELVIS ID Staining Kit
SK-ELVIS-200 200 Tests Ask for price

ELVIS ID Staining Kit
SK-ELVIS-500 500 Tests Ask for price

Hoechst 33342 Staining Kit
abx097207-100l 100 µl
EUR 162.5

Hoechst 33342 Staining Kit
abx097207-1Kit 1 Kit
EUR 276

Hoechst 33342 Staining Kit
abx097207-1ml 1 ml Ask for price

Hoechst 33342 Staining Kit
abx097207-200l 200 µl Ask for price

Hoechst 33342 Staining Kit
MBS826727-01mL 0.1mL
EUR 140

Hoechst 33342 Staining Kit
MBS826727-5x01mL 5x0.1mL
EUR 550

Cell Cycle Staining Kit
KTA2020-100T 100 T
EUR 119
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit
KTA2020-50T 50 T
EUR 69
Description: Abbkine Cell Cycle Staining Kit provides a convenient and accurate determination of the percentage of cells in each phase of the cell cycle.

Cell Cycle Staining Kit
KTA2020-each each Ask for price

Cell Cycle Staining Kit
MBS9718793-100Tests 100Tests
EUR 225

Cell Cycle Staining Kit
MBS9718793-500Tests 500Tests
EUR 355

Cell Cycle Staining Kit
MBS9718793-50Tests 50Tests
EUR 195

Cell Cycle Staining Kit
MBS9718793-5x500Tests 5x500Tests
EUR 1580

Live NucRed staining kit
EGY069 >100T
EUR 210

Live NucRed staining kit
MBS8584964-100Tests 100Tests
EUR 310

Live NucRed staining kit
MBS8584964-5x100Tests 5x100Tests
EUR 1405

Live NucGreen staining kit
EGY068 >100T
EUR 210

Dead NucGreen staining kit
EGY070 >100T
EUR 210

Live NucGreen staining kit
MBS8584963-100Tests 100Tests
EUR 310

Live NucGreen staining kit
MBS8584963-5x100Tests 5x100Tests
EUR 1405

Dead NucGreen staining kit
MBS8584965-100Tests 100Tests
EUR 310

Dead NucGreen staining kit
MBS8584965-5x100Tests 5x100Tests
EUR 1405

AO/PI Double Staining Kit
K2238-100 100T
EUR 40
Description: Cell Biology|Cell Apoptosis

AO/PI Double Staining Kit
K2238-500 500T
EUR 120
Description: Cell Biology|Cell Apoptosis

Dual- Color AP Staining Kit
AP100D-1 100 Assays
EUR 494.4

CellSurface FLOW Staining Kit
MBS668926-1Kit 1Kit
EUR 200

CellSurface FLOW Staining Kit
MBS668926-5x1Kit 5x1Kit
EUR 900

Live/Dead Cell Staining Kit
55R-1434 100 tests
EUR 500
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Red-Color AP Staining Kit
AP100R-1 50 Assays
EUR 314.4

CometAssay Silver Staining Kit
4254-200-K 200 Tests
EUR 390.96

β-Galactosidase Staining Kit
AKR-100 1 kit
EUR 288
Description: Excepted Quantities

Blue-Color AP Staining Kit
AP100B-1 50 Assays
EUR 203

Beta Galactosidase Staining Kit
55R-1541 250 assays
EUR 300
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Stains-all
03943-1 1g
EUR 81
Description: 7423-31-6

Stains-all
03943-5 5g
EUR 277.56
Description: 7423-31-6

Stains-all
581275 1.0g
EUR 230

Stains-All
D6359 1g
EUR 126.82

Stains-All
HY-D0987 200mg
EUR 129.6

Stains-All
GL8815 1g
EUR 202.91

Stains-All
GL8815-1 1
EUR 63.3

Stains-All
GL8815-5 5
EUR 221.5

Stains-All
MBS5755562-5mg 5mg
EUR 145

Stains-All
MBS5755562-5x5mg 5x5mg
EUR 500

Stains-All
T16939-10mg 10mg Ask for price
Description: Stains-All

Stains-All
T16939-1g 1g Ask for price
Description: Stains-All

Stains-All
T16939-1mg 1mg Ask for price
Description: Stains-All

Stains-All
T16939-50mg 50mg Ask for price
Description: Stains-All

Stains-All
T16939-5mg 5mg Ask for price
Description: Stains-All

Live-Dead Cell Staining Kit
GWB-AXR262 100 stainings Ask for price

Live-Dead Cell Staining Kit
K2081-100 100 staining
EUR 552

Live-Dead Cell Staining Kit
K2081-1000 1000T
EUR 124.8
Description: Tools Kit|Cell Staining Kit#Kits|Tools Kit#Kits

Live-Dead Cell Staining Kit
K2081-500 500T
EUR 76
Description: Tools Kit|Cell Staining Kit#Kits|Tools Kit#Kits

Live-Dead Cell Staining Kit
K501-100 each
EUR 561.6

Nerve Terminal Staining Kit I
70030-1 KT
EUR 605

Nerve Terminal Staining Kit V
70034 1SET
EUR 605
Description: N/A

Nerve Terminal Staining Kit V
70034-1 KT
EUR 605

Purple-Color AP Staining Kit
AP100P-1 100 Assays
EUR 235

Human Histochemistry Staining Kit
20-abx299039
  • Ask for price
  • Ask for price
  • Ask for price
  • 50 tests
  • 150 tests
  • 500 tests

Beta-Galactosidase Staining Kit
GWB-AXR372 200 assays. Ask for price

Beta-Galactosidase Staining Kit
K2182-250 250 assays
EUR 422.4
Description: Tools Kit|Molecular Biology Kit#Kits|Tools Kit#Kits

Alkaline Phosphatase Staining Kit
K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit
K802-250 each
EUR 352.8

beta-Galactosidase Staining Kit
MBS168046-1Kit 1Kit
EUR 505

beta-Galactosidase Staining Kit
MBS168046-5x1Kit 5x1Kit
EUR 2320

Lipid (Oil Red O) staining Kit
K580-24 each
EUR 542.4

Nerve Terminal Staining Kit IIA
70031-1 1SET
EUR 322
Description: N/A

Nerve Terminal Staining Kit IIB
70031-1-1 KT
EUR 395

Nerve Terminal Staining Kit III
70032-1 KT
EUR 513

Red Active Caspase Staining Kit
K2052-100 100 assays
EUR 646.4
Description: Kits|Apoptosis Kit#Apoptosis Kit|Caspase Kit#Kits

Red Active Caspase Staining Kit
K2052-25 25 assays
EUR 285.6

QUANTOM Total Cell Staining Kit
LBQ13501 each
EUR 537.92

Alcian Blue (pH 2.5) Staining Kit
AFR-1 250 ml ea.
EUR 127.28

Alcian Blue (pH 2.5) Staining Kit
AFR-2 30 ml ea.
EUR 63

DAPI staining kit (ready to use) 
EGY0621 10mL
EUR 85

DAPI staining kit (ready to use) 
EGY0622 50mL
EUR 185

DAPI staining kit (ready to use) 
MBS8584952-10mL 10mL
EUR 200

DAPI staining kit (ready to use) 
MBS8584952-5x10mL 5x10mL
EUR 915

DAPI staining kit (ready to use) 
MBS8584953-50mL 50mL
EUR 285

DAPI staining kit (ready to use) 
MBS8584953-5x50mL 5x50mL
EUR 1300

Propidium Iodide (PI) Staining Kit
abx097208-100l 100 µl
EUR 162.5

Propidium Iodide (PI) Staining Kit
abx097208-1ml 1 ml Ask for price

Propidium Iodide (PI) Staining Kit
abx097208-200l 200 µl Ask for price

DNA Ploidy Analysis Staining Kit
K1439-1 each
EUR 705.6

DNA Ploidy Analysis Staining Kit
K1439-5 each
EUR 2402.4

QUANTOM Viable Cell Staining Kit
LBQ13502 each
EUR 670.35

Stayrightâ„¢ Purple HRP Staining Kit
45905 5 mL
EUR 112
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit
45905-5mL 5 mL
EUR 109
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit
45906 50 mL
EUR 341
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit
45906-50mL 50 mL
EUR 334
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Hematoxylin and Eosin Staining Kit
K1142-100 100mL
EUR 40
Description: Protein & Immunology|Immunofluorescence & Immunohistochemistry

Hematoxylin and Eosin Staining Kit
K1142-1000 2×500mL
EUR 62
Description: Hematoxylin and Eosin Staining，one of the most widely used staining technique in histopathology

Hematoxylin and Eosin Staining Kit
K1142-500 500mL
EUR 72
Description: Protein & Immunology|Immunofluorescence & Immunohistochemistry

Live-Dead Bacterial Staining Kit
K2239-100 100T
EUR 300.8
Description: Cell Biology|Cell Proliferation & Toxicity

Live-Dead Bacterial Staining Kit
K2239-20 20T
EUR 78.4
Description: Cell Biology|Cell Proliferation & Toxicity

Hochst33342/PI Double Staining Kit
E37F032-100-tests 100tests
EUR 67
Description: Double staining kit

TRAP Staining Kit with Osteoplates
KT-651 1 kit
EUR 1003

Hochst33342/PI Double Staining Kit
MBS8579846-100Tests 100Tests
EUR 170

Hochst33342/PI Double Staining Kit
MBS8579846-5x100Tests 5x100Tests
EUR 735

Calcein AM/PI Double Staining Kit
E-CK-A354-100Assays 100 Assays
EUR 100
Description: Viability/Cytotoxicity

Calcein AM/PI Double Staining Kit
E-CK-A354-2000Assays 2000 Assays
EUR 600
Description: Viability/Cytotoxicity

Calcein AM/PI Double Staining Kit
E-CK-A354-500Assays 500 Assays
EUR 300
Description: Viability/Cytotoxicity

Calcein AM/PI Double Staining Kit
E-CK-A354-each each Ask for price
Description: Viability/Cytotoxicity

Hoechst 33342 / PI Double Staining Kit
abx097209-1Kit 1 Kit
EUR 292.8

Hoechst 33342/PI Double Staining Kit
K2237-100 100T
EUR 40
Description: Cell Biology|Cell Apoptosis

Hoechst 33342/PI Double Staining Kit
K2237-500 500T
EUR 96
Description: Cell Biology|Cell Apoptosis

Hoechst 33342/PI Double Staining Kit
MBS826730-01mL 0.1mL
EUR 160

Hoechst 33342/PI Double Staining Kit
MBS826730-5x01mL 5x0.1mL
EUR 625

EzWay Protein-Silver Staining Kit
K14040D 20A
EUR 188
Description: https://komabiotech.co.kr/labiskoma/datasheet/K14040D-korean.pdf

Protein Stain-EZ J Phosphoprotein Staining Kit
BSP043 2x10minigel, 20prep
EUR 133.08

CaspGLOW Active Caspase Staining Kit
55R-1292 25 assays
EUR 613
Description: Caspase Staining Kit for use activity in the research laboratory

Red Active Caspase-3 Staining Kit
K2053-100 100 assays
EUR 940.8
Description: Kits|Apoptosis Kit#Apoptosis Kit|Caspase Kit#Kits

Red Active Caspase-3 Staining Kit
K2053-25 25 assays
EUR 285.6

Red Active Caspase-8 Staining Kit
K2054-100 100 assays
EUR 755.2
Description: Kits|Apoptosis Kit#Apoptosis Kit|Caspase Kit#Kits

Red Active Caspase-8 Staining Kit
K2054-25 25 assays
EUR 285.6

Red Active Caspase-9 Staining Kit
K2055-100 100 assays
EUR 940.8
Description: Kits|Apoptosis Kit#Apoptosis Kit|Caspase Kit#Kits

Red Active Caspase-9 Staining Kit
K2055-25 25 assays
EUR 285.6

Calcein-AM/PI Double Staining Kit
E37F030-500-tests 500tests
EUR 267
Description: Double staining kit

Calcein-AM/PI Double Staining Kit
E37F031-1000-tests 1000tests
EUR 527
Description: Double staining kit

Calcein-AM/PI Double Staining Kit
MBS2570579-100Assays 100Assays
EUR 215

Calcein-AM/PI Double Staining Kit
MBS2570579-2000Assays 2000Assays
EUR 550

Calcein-AM/PI Double Staining Kit
MBS2570579-500Assays 500Assays
EUR 340

Calcein-AM/PI Double Staining Kit
MBS2570579-5x2000Assays 5x2000Assays
EUR 2515

Calcein-AM/PI Double Staining Kit
MBS8579844-500Tests 500Tests
EUR 305

Calcein-AM/PI Double Staining Kit
MBS8579844-5x500Tests 5x500Tests
EUR 1340

Calcein-AM/PI Double Staining Kit
MBS8579845-1000Tests 1000Tests
EUR 575

Calcein-AM/PI Double Staining Kit
MBS8579845-5x1000Tests 5x1000Tests
EUR 2580

Alkaline Phosphatase Red Staining kit
TBS2080 50 tests
EUR 89

Hoechst 33342 staining kit (ready to use) 
EGY0631 10mL
EUR 50

Hoechst 33342 staining kit (ready to use) 
EGY0632 50mL
EUR 160

In addition, excess miR4435-2HG inhibit the development of OA, indicating that miR4435-2HG can be considered as a potential therapeutic target in OA.

Gina

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