Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux

Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux

Background: Cerebral ischemia-reperfusion (I / R) injury can cause severe dysfunction, and treatment difficult. It is reported that the nucleotide-binding domain and a leucine-rich repeat protein family of 3 (NLRP3) inflammasome-mediated cell pyroptosis an important part of the I / R injury of the brain and can inhibit the activation of autophagy pyroptosis in some tissue injury. Our previous research found that the protective effect of bone marrow mesenchymal stem cells (BMSCs) in brain I / R injury may be associated with the regulation of autophagy.

Recent research has shown that exosomes secreted from BMSCs (BMSC-Exos) may play an important role in effective biological performance BMSCs and BMSC-Exos protective mechanism associated with the activation of autophagy and remission of inflammation, but have not been reported in studies of injury I / R brain. We aimed to investigate the effects of BMSC-Exos on I / R injury of the brain and determine whether the mechanisms associated with the regulation pyroptosis and autophagic flux.

Methods: PC12 cells were subjected to a shortage of oxygen-glucose / reoxygenation (OGD / R) to induce brain I / R in vitro and cocultured with BMSC-Exos. cell viability was determined by CCK-8 kit and lactate dehydrogenase (LDH) detection. Scanning electron microscopy (SEM), Hoechst 33 342 / propidium iodide (PI) double staining, 2 ‘, 7’-dichlorodihydrofluorescein diacetate assay, immunofluorescence, Western blot and enzyme-linked immunosorbent assay (ELISA) was used to detect cell pyroptosis. Furthermore, transmission electron microscopy (TEM), GFP-RFP-LC3 adenovirus transfection, and Western blot used to detect autophagic flux and its influence on pyroptosis.

Finally, coimmunoprecipitation used to detect the binding interaction between NLRP3 and LC3. Results: BMSC-Exos increased cell viability in OGD / R inhibitory effect of BMSC-Exos on pyroptosis is comparable to NLRP3 inhibitor NLRP3 MCC950 and reversed by excess. Furthermore, BMSC-Exos promoted autophagic flux through AMP-activated kinase (AMPK) / mammalian target of rapamycin on the track, whereas chloroquine, AMPK silencing, and compound C block the inhibitory effects on pyroptosis. Conclusion: BMSC-Exos can protect PC12 cells against injury OGD / R by the weakening of the NLRP3 inflammasome-mediated pyroptosis to promote the AMPK-dependent autophagic flux.

 Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux
Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux

Effect of Morinda citrifolia (Noni) on Cell Viability of Stem Cells and Osteogenesis spheroids

Background and purpose: Morinda citrifolia (Noni) has been widely used in herbal medicine to treat and prevent various diseases. We undertook this study to evaluate the effects of Noni extract on morphology maintenance, increase cellular viability, and improved osteogenesis of stem cell spheroids.

Materials and Methods: We cultured stem cell spheroids made with gingival-derived stem cells in the presence of Noni extract at concentrations of 10, 100 and 200 ng / mL. We do the analysis of changes in cell morphology and cell viability. We did a test alkaline phosphatase activity using a kit and test the mineralization using anthraquinone dye to evaluate osteogenesis of stem cell spheroids with the addition of Noni extract.

Trap 101

B7084-10 10 mg
EUR 373

Trap 101

B7084-50 50 mg
EUR 1363

Rat(TRAP)

QY-E11743 96T
EUR 374

TRAP-5

H-1408.0005 5.0mg
EUR 576
Description: Sum Formula: C30H50N8O7; CAS# [141685-53-2] net

TRAP-5

H-1408.0025 25.0mg
EUR 2207
Description: Sum Formula: C30H50N8O7; CAS# [141685-53-2] net

TRAP-7

H-2234.0005 5.0mg
EUR 539
Description: Sum Formula: C39H63N11O10; CAS# [145229-76-1] net

TRAP-7

H-2234.0025 25.0mg
EUR 2061
Description: Sum Formula: C39H63N11O10; CAS# [145229-76-1] net

TRAP-14

H-8105.0001 1.0mg
EUR 151
Description: Sum Formula: C81H118N20O23; CAS# [137339-65-2] net

TRAP-14

H-8105.0005 5.0mg
EUR 515
Description: Sum Formula: C81H118N20O23; CAS# [137339-65-2] net

TRAP-6

H-8365.0005 5.0mg
EUR 273
Description: Sum Formula: C34H56N10O9; CAS# [141136-83-6] net

TRAP-6

H-8365.0025 25.0mg
EUR 998
Description: Sum Formula: C34H56N10O9; CAS# [141136-83-6] net

TRAP-6

H-7764.0500 0.5mg
EUR 48
Description: Sum Formula: C34H56N10O9; CAS# [141136-83-6] net

TRAP-6

H-7764.1000 1.0mg
EUR 60
Description: Sum Formula: C34H56N10O9; CAS# [141136-83-6] net

TRAP-6

A8675-5 5 mg
EUR 173
Description: Peptide fragment (residues 42-47) of protease-activated receptor 1 (PAR1) that acts as a PAR1 agonist. Stimulates platelet aggregation (EC50 = 0.8 ?M), promotes intracellular Ca2+ mobilization and induces rapid phosphodiesterase 3A (PDE3A) phosphorylation

TRAP Peptide

6911P 0.05 mg
EUR 164.75
Description: (CT) TRAP peptide

TRAP -6

ABP-TRA-1 5,0mg vial
EUR 170

TRAP antibody

70R-12535 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal TRAP antibody

TRAP Antibody

6911-002mg 0.02 mg
EUR 171.82
Description: TRAP Antibody: TRAP, also known as uteroferrin, is an iron containing, glycosylated, acid phosphatase. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. Along with the related protein ACP2, TRAP mediates the removal of mannose 6-phosphate residues from proteins targeted to lysosomes. TRAP is present in brain at low levels, but is expressed at a much higher level in liver.

TRAP Antibody

6911-01mg 0.1 mg
EUR 436.42
Description: TRAP Antibody: TRAP, also known as uteroferrin, is an iron containing, glycosylated, acid phosphatase. It is the most basic of the acid phosphatases and is the only form not inhibited by L(+)-tartrate. Along with the related protein ACP2, TRAP mediates the removal of mannose 6-phosphate residues from proteins targeted to lysosomes. TRAP is present in brain at low levels, but is expressed at a much higher level in liver.

?-Galactosidase Staining Kit

AKR-100 1 kit
EUR 432
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 230

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 230

Sheep TRAP ELISA Kit

EST0544 96Tests
EUR 521

Rat TRAP ELISA Kit

ERT0544 96Tests
EUR 521

Monkey TRAP ELISA Kit

EMKT0544 96Tests
EUR 521

Mouse TRAP ELISA Kit

EMT0544 96Tests
EUR 521

Porcine TRAP ELISA Kit

EPT0544 96Tests
EUR 521

Goat TRAP ELISA Kit

EGTT0544 96Tests
EUR 521

Human TRAP ELISA Kit

EHT0544 96Tests
EUR 521

Bovine TRAP ELISA Kit

EBT0544 96Tests
EUR 521

Chicken TRAP ELISA Kit

ECKT0544 96Tests
EUR 521

Canine TRAP ELISA Kit

ECT0544 96Tests
EUR 521

Mouse TRAP ELISA Kit

ELI-03023m 96 Tests
EUR 865

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 230

Human Histochemistry Staining Kit

20-abx299039
  • EUR 871.00
  • EUR 1219.00
  • EUR 565.00
  • 150 tests
  • 500 tests
  • 50 tests

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 325.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 293

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 370
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 379

Beta-Galactosidase Staining Kit

K802-250
EUR 294

Anti-TRAP antibody

STJ190098 200 µl
EUR 197
Description: Unconjugated Mouse monoclonal to TRAP (2H2)

Polyclonal TRAP Antibody

APR10538G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TRAP . This antibody is tested and proven to work in the following applications:

CD40 Ligand/TRAP

PR29002 50 ug
EUR 491

[Cit5]-TRAP-5

5-00062 4 x 5mg Ask for price

[Tyr1]-TRAP-7

5-00339 4 x 5mg Ask for price

Scrambled TRAP Fragment

5-01910 4 x 5mg Ask for price

TRAP-14 amide

5-02018 4 x 1mg Ask for price

TRAP-5 amide

5-02019 4 x 5mg Ask for price

TRAP-14 Peptide

20-abx266463
  • EUR 551.00
  • EUR 926.00
  • EUR 398.00
  • 10 mg
  • 25 mg
  • 5 mg

TRAP Conjugated Antibody

C36331 100ul
EUR 397

TRAP Conjugated Antibody

C38129 100ul
EUR 397

(Tyr1)-TRAP-7

H-1674.0001 1.0mg
EUR 126
Description: Sum Formula: C45H67N11O10; CAS# [149440-16-4] net

(Tyr1)-TRAP-7

H-1674.0005 5.0mg
EUR 418
Description: Sum Formula: C45H67N11O10; CAS# [149440-16-4] net

TRAP-6 amide

H-2936.0005 5.0mg
EUR 115
Description: Sum Formula: C34H57N11O8; CAS# [141923-40-2] net

TRAP-6 amide

H-2936.0025 25.0mg
EUR 368
Description: Sum Formula: C34H57N11O8; CAS# [141923-40-2] net

TRAP-5 amide

H-2938.0025 25.0mg
EUR 539
Description: Sum Formula: C30H51N9O6; CAS# [141923-41-3] net

TRAP-5 amide

H-2938.0100 100.0mg
EUR 1554
Description: Sum Formula: C30H51N9O6; CAS# [141923-41-3] net

TRAP-14 amide

H-6032.0001 1.0mg
EUR 212
Description: Sum Formula: C81H119N21O22; CAS# [141923-36-6] net

TRAP-14 amide

H-6032.0005 5.0mg
EUR 756
Description: Sum Formula: C81H119N21O22; CAS# [141923-36-6] net

TRAP Conjugated Antibody

C44711 100ul
EUR 397

anti-TRAP alpha

YF-PA14790 50 ul
EUR 363
Description: Mouse polyclonal to TRAP alpha

anti-TRAP alpha

YF-PA14791 50 ug
EUR 363
Description: Mouse polyclonal to TRAP alpha

Guinea Pig TRAP ELISA Kit

EGT0544 96Tests
EUR 521

Cell Navigator™ Lysosome Staining Kit *Green Fluorescence with 405 nm Excitation*

22651 500 Tests
EUR 219

Cell Navigator™ Mitochondrion Staining Kit *Orange Fluorescence with 405 nm Excitation*

22673 500 Assays
EUR 219

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132

ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody

EXOAB-KIT-1 25 ul each
EUR 627

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2132

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132

Nerve Terminal Staining Kit V

70034 1SET
EUR 509
Description: Minimum order quantity: 1 unit of 1SET

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 238

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 572

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 238

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 460

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 262

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 412

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 262

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 638
Description: Live/Dead Cell Staining Kit for use in the research laboratory

DNA Ploidy Analysis Staining Kit

K1439-1
EUR 588

DNA Ploidy Analysis Staining Kit

K1439-5
EUR 2002

Live-Dead Cell Staining Kit

K501-100
EUR 468

Anti-TRAP Monoclonal Antibody

M03277-1 100ug
EUR 397
Description: Rabbit Monoclonal TRAP Antibody. Validated in IP, IF, IHC, ICC, WB and tested in Human, Mouse, Rat.

CD40 Ligand/TRAP (Soluble)

PR27033 50 ug
EUR 507

[Phe1, Ser2]-TRAP-6

5-00263 4 x 5mg Ask for price

TRAP-6 (2-6)

5-02020 4 x 5mg Ask for price

TRAP-5 amide Peptide

20-abx265852
  • EUR 356.00
  • EUR 537.00
  • EUR 286.00
  • 10 mg
  • 25 mg
  • 5 mg

Scrambled TRAP Fragment Peptide

20-abx265858
  • EUR 356.00
  • EUR 537.00
  • EUR 286.00
  • 10 mg
  • 25 mg
  • 5 mg

[Tyr1]-TRAP-7 Peptide

20-abx265889
  • EUR 356.00
  • EUR 537.00
  • EUR 286.00
  • 10 mg
  • 25 mg
  • 5 mg

[Cit5]-TRAP-5 Peptide

20-abx265945
  • EUR 384.00
  • EUR 592.00
  • EUR 300.00
  • 10 mg
  • 25 mg
  • 5 mg

(DL-Isoser1)-TRAP-6

H-1944.0001 1.0mg
EUR 273
Description: Sum Formula: C34H56N10O9; CAS# [150242-29-8] net

(DL-Isoser1)-TRAP-6

H-1944.0005 5.0mg
EUR 998
Description: Sum Formula: C34H56N10O9; CAS# [150242-29-8] net

(Phe1,Ser2)-TRAP-6

H-5996.0005 5.0mg
EUR 297
Description: Sum Formula: C34H56N10O9; CAS# [374898-11-0] net

(Phe1,Ser2)-TRAP-6

H-5996.0025 25.0mg
EUR 1093
Description: Sum Formula: C34H56N10O9; CAS# [374898-11-0] net

TRAP-6 (2-6)

H-8325.0005 5.0mg
EUR 273
Description: Sum Formula: C31H51N9O7; CAS# [141136-84-7] net

TRAP-6 (2-6)

H-8325.0025 25.0mg
EUR 998
Description: Sum Formula: C31H51N9O7; CAS# [141136-84-7] net

Anti-TRAP alpha (1A12)

YF-MA10883 100 ug
EUR 363
Description: Mouse monoclonal to TRAP alpha

Anti-TRAP alpha (1C11)

YF-MA15622 100 ug
EUR 363
Description: Mouse monoclonal to TRAP alpha

ACP5/TRAP, human recombinant

P1055-10
EUR 164

ACP5/TRAP, human recombinant

P1055-50
EUR 588

CD40Ligand/TRAP, human recombinant

4014-10
EUR 137

CD40Ligand/TRAP, human recombinant

4014-1000
EUR 1626

CD40Ligand/TRAP, human recombinant

4014-50
EUR 256

CD40Ligand/TRAP, murine recombinant

4015-100
EUR 642

CD40Ligand/TRAP, murine recombinant

4015-1000
EUR 3149

CD40Ligand/TRAP, murine recombinant

4015-25
EUR 245

CD40Ligand/TRAP, murine recombinant

4015-500
EUR 1953

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

Screen Quest™ 10X cell staining buffer with Phenol Red Plus™

36300 10 Plates
EUR 132

PCR Mycoplasma Detection Kit

M034-Kit Kit
EUR 266

Human Type I Collagen Staining Kit

9044 1 kit
EUR 338.55
Description: Human Type I Collagen Staining Kit

Yeast Viability Staining Kit (Green/Red)

31063-1 1kit
EUR 472
Description: Minimum order quantity: 1 unit of 1kit

StemTAG Alkaline Phosphatase Staining Kit (Red)

CBA-300 100 assays
EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

StemTAG Alkaline Phosphatase Staining Kit (Purple)

CBA-306 100 assays
EUR 461
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
EUR 189

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 244

Human FoxP3 Transcription Factor Staining Kit

OKTA00001 KIT
EUR 454
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Human FoxP3 Transcription Factor Staining Kit

OKTA00004 KIT
EUR 454
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Fluorescein Active Caspase-12 Staining Kit

K2046-25 25 assays
EUR 238

Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
EUR 238

Fluorescein Active Caspase-3 Staining Kit

K2049-25 25 assays
EUR 238

Fluorescein Active Caspase-8 Staining Kit

K2050-25 25 assays
EUR 238

Fluorescein Active Caspase-9 Staining Kit

K2051-100 100 assays
EUR 572

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 238

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 572

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 238

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 572

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 238

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 572

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 238

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 208

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 128

CaspGLOW Active Caspase 12 Staining Kit

55R-1291 25 assays
EUR 282
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit

55R-1293 25 assays
EUR 284
Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit

55R-1294 25 assays
EUR 296
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit

55R-1295 25 assays
EUR 296
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 9 Staining Kit

55R-1296 25 assays
EUR 296
Description: Caspase 9 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase Staining Kit

55R-1297 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

Genorise® Alkaline Phosphatase Staining Kit

GR107045 100 tests
EUR 289

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-100
EUR 550

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-25
EUR 234

CaspGLOW? Red Active Caspase Staining Kit

K190-100
EUR 523

CaspGLOW? Red Active Caspase Staining Kit

K190-25
EUR 240

Lipid (Oil Red O) staining Kit

K580-24
EUR 452

Gel-FAST? Gel Staining/Destaining Kit

K901-40
EUR 175

sCD40 Ligand, TRAP, rHusCD40L, human

RC214-17 50ug
EUR 152.66

Polyclonal TRAP Antibody (N-term)

APR05766G 0.1ml
EUR 484
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TRAP (N-term). This antibody is tested and proven to work in the following applications:

N-10 Region of TRAP

5-01576 4 x 5mg Ask for price

soluble CD40 Ligand / TRAP Protein

20-abx260376
  • EUR 230.00
  • EUR 1859.00
  • EUR 328.00
  • 10 ug
  • 1 mg
  • 50 ug

soluble CD40 Ligand / TRAP Protein

20-abx260744
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 20 ug
  • 5 ug

Soluble CD40 Ligand / TRAP Protein

20-abx261655
  • EUR 3418.00
  • EUR 328.00
  • EUR 230.00
  • 1 mg
  • 25 ug
  • 5 ug

TRAP-6 (2-6) Peptide

20-abx265781
  • EUR 328.00
  • EUR 495.00
  • EUR 272.00
  • 10 mg
  • 25 mg
  • 5 mg

[Phe1,Ser2]-TRAP-6 Peptide

20-abx265794
  • EUR 328.00
  • EUR 495.00
  • EUR 272.00
  • 10 mg
  • 25 mg
  • 5 mg

Ponceau S Staining Solution

PW005 100ml
EUR 69.14

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 174

PI / RNase Staining Buffer

20-abx090619
  • EUR 272.00
  • EUR 230.00
  • 100 tests
  • 50 tests

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 176

Ponceau S Staining Solution

B7778-100000 100 g
EUR 163

Ponceau S Staining Solution

B7778-50000 50 g
EUR 126

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 58

Live or Dead™ Fixable Dead Cell Staining Kit *Blue Fluorescence with 405 nm Excitation*

22500 200 Tests
EUR 176

Live or Dead™ Fixable Dead Cell Staining Kit *Green Fluorescence with 405 nm Excitation*

22501 200 Tests
EUR 176

Live or Dead™ Fixable Dead Cell Staining Kit *Orange Fluorescence with 405 nm Excitation*

22502 200 Tests
EUR 176

Cas9 Protein and T7 gRNA SmartNuclease Synthesis Kit

CAS400A-KIT 1 kit (10 rxn)
EUR 1110

AXYPET STARTER KIT 1 AP-20, AP-200 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS

AP-STR-KIT-1 1/pk
EUR 355
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

AXYPET STARTER KIT 2 AP-10, AP-100 & AP-1000 WITH ADDITIONAL FREE RACKS OF AXYGEN PIPETTE TIPS

AP-STR-KIT-2 1/pk
EUR 367
Description: Corning and Axygen Liquid Handling Equipment; Axypet Pipettors and Motopet Pipet Controller

CMV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV100PA-KIT 1 Kit
EUR 1132

CMV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV105PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-T2A-Puro SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV120PA-KIT 1 Kit
EUR 1132

MSCV-hspCas9-EF1-GFP SmartNuclease Lentivector Plasmid + LentiStarter Packaging Kit

CASLV125PA-KIT 1 Kit
EUR 1132

Multiplex gRNA Kit + EF1-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS700A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CAG-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS720A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + CMV-T7-hspCas9-H1-gRNA linearized SmartNuclease vector

CAS740A-KIT 10 rxn
EUR 1132

T7 gRNA SmartNuclease Synthesis Kit (includes CAS510A-1 & T7 IVT synthesis reagents)

CAS510A-KIT 1 Kit
EUR 805

Cas9 Nickase: CMV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV200PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: CMV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV205PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-T2A-Puro SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV220PA-KIT 1 Kit
EUR 1132

Cas9 Nickase: MSCV-hspCas9(D10A)-EF1-GFP SmartNickase Lentivector Plasmid + LentiStarter Packaging Kit

CASLV225PA-KIT 1 Kit
EUR 1132

Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit

CSB-E13415h-24T 1 plate of 24 wells
EUR 165
Description: Quantitativesandwich ELISA kit for measuring Human Tartrate Resistant Acid Phosphatase (TRAP) in samples from serum, plasma, tissue homogenates. A new trial version of the kit, which allows you to test the kit in your application at a reasonable price.

Human Tartrate Resistant Acid Phosphatase(TRAP)ELISA Kit

1-CSB-E13415h
  • EUR 900.00
  • EUR 5476.00
  • EUR 2900.00
  • 1 plate of 96 wells
  • 10 plates of 96 wells each
  • 5 plates of 96 wells each
Description: Quantitativesandwich ELISA kit for measuring Human Tartrate Resistant Acid Phosphatase(TRAP) in samples from serum, plasma, tissue homogenates. Now available in a cost efficient pack of 5 plates of 96 wells each, conveniently packed along with the other reagents in 5 separate kits.

Bovine Tartrate Resistant Acid Phosphatase (TRAP) ELISA Kit

QY-E60105 96T
EUR 426

Canine tartrate-resistant acid phosphatase (TRAP) ELISA Kit

QY-E70105 96T
EUR 426

Rabbit tartrate-resistant acid phosphatase (TRAP))ELISA Kit

QY-E30017 96T
EUR 374

Multiplex gRNA Kit + Cas9 Nickase: EF1-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS750A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CAG-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS770A-KIT 10 rxn
EUR 1132

Multiplex gRNA Kit + Cas9 Nickase: CMV-T7-hspCas9-nickase-H1-gRNA linearized SmartNickase vector

CAS790A-KIT 10 rxn
EUR 1132

Type IX Collagen Staining Kit, Multi-Species

9012 1 kit
EUR 338.55
Description: Type IX Collagen Staining Kit

Sirius Red/Fast Green Collagen Staining Kit

9046 30-50 samples
EUR 137
Description: Sirius Red/Fast Green Collagen Staining Kit

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230 50 assays
EUR 519
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Results: The cells formed spheroids well applied, and the addition of Noni in 10, 100 and 200 concentration ng / mL did not produce significant morphological changes. Quantitative values ​​for the survival of the mobile on day 3 showed that the values ​​of absorbance at 450 nm is 0.314 ± 0.013, 0.318 ± 0.008, 0.304 ± 0.000 and 0.300 ± 0.011 for Noni at 0, 10, 100 and 200 concentration ng / mL , each.

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