Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux

Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux

Background: Cerebral ischemia-reperfusion (I / R) injury can cause severe dysfunction, and treatment difficult. It is reported that the nucleotide-binding domain and a leucine-rich repeat protein family of 3 (NLRP3) inflammasome-mediated cell pyroptosis an important part of the I / R injury of the brain and can inhibit the activation of autophagy pyroptosis in some tissue injury. Our previous research found that the protective effect of bone marrow mesenchymal stem cells (BMSCs) in brain I / R injury may be associated with the regulation of autophagy.

Recent research has shown that exosomes secreted from BMSCs (BMSC-Exos) may play an important role in effective biological performance BMSCs and BMSC-Exos protective mechanism associated with the activation of autophagy and remission of inflammation, but have not been reported in studies of injury I / R brain. We aimed to investigate the effects of BMSC-Exos on I / R injury of the brain and determine whether the mechanisms associated with the regulation pyroptosis and autophagic flux.

Methods: PC12 cells were subjected to a shortage of oxygen-glucose / reoxygenation (OGD / R) to induce brain I / R in vitro and cocultured with BMSC-Exos. cell viability was determined by CCK-8 kit and lactate dehydrogenase (LDH) detection. Scanning electron microscopy (SEM), Hoechst 33 342 / propidium iodide (PI) double staining, 2 ‘, 7’-dichlorodihydrofluorescein diacetate assay, immunofluorescence, Western blot and enzyme-linked immunosorbent assay (ELISA) was used to detect cell pyroptosis. Furthermore, transmission electron microscopy (TEM), GFP-RFP-LC3 adenovirus transfection, and Western blot used to detect autophagic flux and its influence on pyroptosis.

Finally, coimmunoprecipitation used to detect the binding interaction between NLRP3 and LC3. Results: BMSC-Exos increased cell viability in OGD / R inhibitory effect of BMSC-Exos on pyroptosis is comparable to NLRP3 inhibitor NLRP3 MCC950 and reversed by excess. Furthermore, BMSC-Exos promoted autophagic flux through AMP-activated kinase (AMPK) / mammalian target of rapamycin on the track, whereas chloroquine, AMPK silencing, and compound C block the inhibitory effects on pyroptosis. Conclusion: BMSC-Exos can protect PC12 cells against injury OGD / R by the weakening of the NLRP3 inflammasome-mediated pyroptosis to promote the AMPK-dependent autophagic flux.

 Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux
Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux

Effect of Morinda citrifolia (Noni) on Cell Viability of Stem Cells and Osteogenesis spheroids

Background and purpose: Morinda citrifolia (Noni) has been widely used in herbal medicine to treat and prevent various diseases. We undertook this study to evaluate the effects of Noni extract on morphology maintenance, increase cellular viability, and improved osteogenesis of stem cell spheroids.

Materials and Methods: We cultured stem cell spheroids made with gingival-derived stem cells in the presence of Noni extract at concentrations of 10, 100 and 200 ng / mL. We do the analysis of changes in cell morphology and cell viability. We did a test alkaline phosphatase activity using a kit and test the mineralization using anthraquinone dye to evaluate osteogenesis of stem cell spheroids with the addition of Noni extract.

Cell Navigator® Lysosome Staining Kit *Green Fluorescence with 405 nm Excitation*

22651 500 Tests
EUR 222

PI Staining Kit

abx097208-1Kit 1 Kit
EUR 276

Cell Navigator® Lysosome Staining Kit *Green Fluorescence with 405 nm Excitation*

22651-500Tests 500 Tests
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

DAPI Staining Kit

abx097206-1Kit 1 Kit
EUR 276

Cell Navigator® Mitochondrion Staining Kit *Orange Fluorescence with 405 nm Excitation*

22673 500 Assays
EUR 222

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Blue Fluorescence with 405 nm Excitation*

22500-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Cell Navigator® Mitochondrion Staining Kit *Orange Fluorescence with 405 nm Excitation*

22673-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Green Fluorescence with 405 nm Excitation*

22501-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Orange Fluorescence with 405 nm Excitation*

22502-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Live or Dead™ Fixable Dead Cell Staining Kit *Blue Fluorescence with 405 nm Excitation*

22500 200 Tests
EUR 166

Live or Dead™ Fixable Dead Cell Staining Kit *Green Fluorescence with 405 nm Excitation*

22501 200 Tests
EUR 166

Screen Questâ„¢ 10X cell staining buffer with Phenol Red Plusâ„¢

36300-10mL 10 mL
EUR 109
Description: Ready-to-use buffer that is optimized for fluorescence cell imaing.

Live or Dead™ Fixable Dead Cell Staining Kit *Orange Fluorescence with 405 nm Excitation*

22502 200 Tests
EUR 166

?-Galactosidase Staining Kit

AKR-100 1 kit
EUR 518.4
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 276

Screen Quest™ 10X cell staining buffer with Phenol Red Plus™

36300 10 Plates
EUR 109

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 390.96

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 494.4

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 444
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 314.4

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 765.6
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 203

Human Histochemistry Staining Kit

20-abx299039
  • EUR 1045.20
  • EUR 1462.80
  • EUR 678.00
  • 150 tests
  • 500 tests
  • 50 tests

Beta-Galactosidase Staining Kit

K802-250 each
EUR 352.8

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 454.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 351.6

Live-Dead Cell Staining Kit

K501-100 each
EUR 561.6

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 552

Purple-Color AP Staining Kit

AP100P-1 100 Assays
EUR 235

Nerve Terminal Staining Kit V

70034 1SET
EUR 610.8
Description: Minimum order quantity: 1 unit of 1SET

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 686.4

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 285.6

STAT Double Staining ragent for Double staining Mo

NB330DB 25 ml
EUR 595
Description: STAT Double Staining ragent for Double staining Mouse and Sheep antibodies, 450 Plus slides

STAT Double Staining reagent for Double staining o

NB326DB 25 ml
EUR 595
Description: STAT Double Staining reagent for Double staining of Mouse and Rabbit antibodies, 450 plus slides

STAT Double Staining Reagent for Double staining o

NB327DB 25 ml
EUR 595
Description: STAT Double Staining Reagent for Double staining of Mouse and Goat antibodies, 450 plus slides

DNA Ploidy Analysis Staining Kit

K1439-1 each
EUR 705.6

DNA Ploidy Analysis Staining Kit

K1439-5 each
EUR 2402.4

Lipid (Oil Red O) staining Kit

K580-24 each
EUR 542.4

Alcian Blue (pH 2.5) Staining Kit

AFR-1 250 ml ea.
EUR 249.6

Alcian Blue (pH 2.5) Staining Kit

AFR-2 30 ml ea.
EUR 153.6

Human FoxP3 Transcription Factor Staining Kit

OKTA00001 KIT
EUR 544.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Human FoxP3 Transcription Factor Staining Kit

OKTA00004 KIT
EUR 544.8
Description: Description of target: ;Species reactivity: ;Application: FC;Assay info: ;Sensitivity:

Stayrightâ„¢ Purple HRP Staining Kit

45905-5mL 5 mL
EUR 109
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

Stayrightâ„¢ Purple HRP Staining Kit

45906-50mL 50 mL
EUR 334
Description: 3,3'-Diaminobenzidine (DAB) has been applied for decades as the most commonly used IHC chromogen because it is inexpensive and sensitive for routine applications.

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 355.2
Description: Caspase Staining Kit for use activity in the research laboratory

Hoechst 33342 / PI Double Staining Kit

abx097209-1Kit 1 Kit
EUR 292.8

Human Type I Collagen Staining Kit

9044 1 kit
EUR 406.26
Description: Human Type I Collagen Staining Kit

Red Active Caspase-3 Staining Kit

K2053-100 100 assays
EUR 686.4

Red Active Caspase-3 Staining Kit

K2053-25 25 assays
EUR 285.6

Red Active Caspase-8 Staining Kit

K2054-100 100 assays
EUR 686.4

Red Active Caspase-8 Staining Kit

K2054-25 25 assays
EUR 285.6

Red Active Caspase-9 Staining Kit

K2055-100 100 assays
EUR 686.4

Red Active Caspase-9 Staining Kit

K2055-25 25 assays
EUR 285.6

Stayright™ Purple HRP Staining Kit

45905 5 mL
EUR 109

Stayright™ Purple HRP Staining Kit

45906 50 mL
EUR 334

Fluorescein Active Caspase Staining Kit

K2047-100 100 assays
EUR 686.4

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 285.6

Yeast Viability Staining Kit (Green/Red)

31063-1 1kit
EUR 566.4
Description: Minimum order quantity: 1 unit of 1kit

CaspGLOW Active Caspase 12 Staining Kit

55R-1291 25 assays
EUR 338.4
Description: Caspase 12 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 2 Staining Kit

55R-1293 25 assays
EUR 340.8
Description: Caspase 2 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 3 Staining Kit

55R-1294 25 assays
EUR 355.2
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 8 Staining Kit

55R-1295 25 assays
EUR 355.2
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Active Caspase 9 Staining Kit

55R-1296 25 assays
EUR 355.2
Description: Caspase 9 Staining Kit for use activity in the research laboratory

Stain Tray Slide Staining System

M920-1 1 Clear
EUR 513.6

Stain Tray Slide Staining System

M920-2 1 Black
EUR 534

CaspGLOW Red Active Caspase Staining Kit

55R-1297 25 assays
EUR 355.2
Description: Caspase Staining Kit for use activity in the research laboratory

CaspGLOW? Red Active Caspase Staining Kit

K190-100 each
EUR 627.6

CaspGLOW? Red Active Caspase Staining Kit

K190-25 each
EUR 288

Coomassie Brilliant Blue Gel Staining Kit

abx090653-1Kit 1 Kit
EUR 226.8

Disposable Slide Staining Trays

MIC5070 PK4
EUR 90

Fluorescein Active Caspase-2 Staining Kit

K2048-100 100 assays
EUR 686.4

Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
EUR 285.6

Fluorescein Active Caspase-3 Staining Kit

K2049-100 100 assays
EUR 686.4

Fluorescein Active Caspase-3 Staining Kit

K2049-25 25 assays
EUR 285.6

Fluorescein Active Caspase-8 Staining Kit

K2050-100 100 assays
EUR 686.4

Fluorescein Active Caspase-8 Staining Kit

K2050-25 25 assays
EUR 285.6

Fluorescein Active Caspase-9 Staining Kit

K2051-100 100 assays
EUR 686.4

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 285.6

Fluorescein Active Caspase-12 Staining Kit

K2046-100 100 assays
EUR 686.4

Fluorescein Active Caspase-12 Staining Kit

K2046-25 25 assays
EUR 285.6

Protein Stain-EZ J Phosphoprotein Staining Kit

BSP043 2x10minigel, 20prep
EUR 133.08

StemTAG Alkaline Phosphatase Staining Kit (Red)

CBA-300 100 assays
EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Genorise® Alkaline Phosphatase Staining Kit

GR107045 100 tests
EUR 289

Prosi Silver Staining Kit for 25 mini gel

S3200-020 1kit
EUR 433.2

CaspGLOW Red Active Caspase 3 Staining Kit

55R-1298 25 assays
EUR 355.2
Description: Caspase 3 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 8 Staining Kit

55R-1299 25 assays
EUR 355.2
Description: Caspase 8 Staining Kit for use activity in the research laboratory

CaspGLOW Red Active Caspase 9 Staining Kit

55R-1300 25 assays
EUR 355.2
Description: Caspase 9 Staining Kit for use activity in the research laboratory

Geliteâ„¢ Green Nucleic Acid Gel Staining Kit

17589-1Kit 1 Kit
EUR 222
Description: Gelite™ Green is a sensitive fluorescent nucleic acid gel stain for detecting nucleic acids in agarose and polyacrylamide gels.

Sirius Red/Fast Green Collagen Staining Kit

9046 30-50 samples
EUR 164.4
Description: Sirius Red/Fast Green Collagen Staining Kit

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-100 each
EUR 639.6

CaspGLOW? Red Active Caspase-3 Staining Kit

K193-25 each
EUR 288

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-100 each
EUR 620.4

CaspGLOW? Red Active Caspase-9 Staining Kit

K199-25 each
EUR 288

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-100 each
EUR 627.6

CaspGLOW? Red Active Caspase-8 Staining Kit

K198-25 each
EUR 288

Geliteâ„¢ Orange Nucleic Acid Gel Staining Kit

17594-1Kit 1 Kit
EUR 222
Description: Gelite™ Orange is an extremely sensitive nucleic acid gel stain for detecting DNA or RNA in gels using a standard 300 nm UV transilluminator and Polaroid 667 black-and-white print film.

StemTAG Alkaline Phosphatase Staining Kit (Purple)

CBA-306 100 assays
EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-25 each
EUR 280.8

Type IX Collagen Staining Kit, Multi-Species

9012 1 kit
EUR 406.26
Description: Type IX Collagen Staining Kit

Blue Feulgen DNA Ploidy Analysis Staining Kit

DPK500 1 kit(s)
EUR 427.2

PI / RNase Staining Buffer

20-abx090619
  • EUR 326.40
  • EUR 276.00
  • 100 tests
  • 50 tests

Gel-FAST? Gel Staining/Destaining Kit

K901-40 each
EUR 210

Gelite™ Green Nucleic Acid Gel Staining Kit

17589 1 Kit
EUR 222

Live-or-Dye NuCFix Red Staining Kit (200 assays)

32010 1kit
EUR 326.4
Description: Minimum order quantity: 1 unit of 1kit

Gelite™ Orange Nucleic Acid Gel Staining Kit

17594 1 Kit
EUR 222

Cell Navigator® Live Cell Tubulin Staining Kit

23170 100 Slides
EUR 222

Cell Navigator® Live Cell Tubulin Staining Kit

23171 300 Slides
EUR 446

BRAND(TM) STAINING TRAY FOR 25 SLIDES

BR471400-5EA PK5
EUR 58.1

BRAND(TM) STAINING TRAY FOR 20 SLIDES

BR474305-2EA PK2
EUR 43.2

EZCell? Intracellular Zinc Staining Kit (Cell-Based)

K525-50 each
EUR 679.2

Cell Navigator® Live Cell Tubulin Staining Kit

23170-100Slides 100 Slides
EUR 222
Description: Cell Navigator® Live Cell Tubulin Staining Kit provides a robust method to fluorescently image tubulins in live cells with Tubulite™ Red.

Cell Navigator® Live Cell Tubulin Staining Kit

23171-300Slides 300 Slides
EUR 446
Description: Cell Navigator® Live Cell Tubulin Staining Kit provides a robust method to fluorescently image tubulins in live cells with Tubulite™ Red.

Ponceau S Staining Solution

B7778-100000 100 g
EUR 195.6

Ponceau S Staining Solution

B7778-50000 50 g
EUR 151.2

SDS-PAGE Staining Solution

GR103068 100 mL
EUR 58

Ponceau S Staining Solution

PW005 100ml
EUR 82.97

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-25 each
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-100 each
EUR 627.6

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-25 each
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-25 each
EUR 288

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-25 each
EUR 288

EasyDip Slide Staining System

M900-12AS-ASSORTED 1 Case(s)
EUR 211.2

Lysosomal Cytotoxicity Dual Staining Kit (Cell-Based)

K458-50 each
EUR 686.4

Autophagy/Cytotoxicity Dual Staining Kit (Cell-Based)

K449-50 each
EUR 652.8

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-100 each
EUR 627.6

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-25 each
EUR 280.8

PAP PEN FOR IMMUNO STAINING

Z377821-1EA EACH
EUR 171.03

Nalgene Box Staining 13x13x5cm

BOX1040 EACH
EUR 48

OxiSelect DNA Double-Strand Break (DSB) Staining Kit

STA-321 100 assays
EUR 790.8
Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.

Staining Trough+Lid Rectangular

MIC6008 EACH
EUR 27.6

ProsiBlue Gel Staining Solution

P7300-050 500ml
EUR 208.8

Wheaton staining dishes 3 EA

S5017-3EA PK3
EUR 135.6

CaspGLOW™ Green Active Caspase-3/7 Staining Kit

K2030-25 25 assays
EUR 272.4

Staining Jar PP + Lid 10 Slide

MIC6002 EACH
EUR 67.2

Staining Jar W Lid 115x115x77mm

MIC6016 PK10
EUR 428.4

Manual Staining System (12 Wells)

IMS001 1 ea.
EUR 427.2

Cell Navigator® Lysosome Staining Kit *NIR Fluorescence*

22652 500 Tests
EUR 222

Cell Navigator® Lysosome Staining Kit *Red Fluorescence*

22658 500 Tests
EUR 166

Cell Navigator® Lysosome Staining Kit *Blue Fluorescence*

22655 500 Tests
EUR 166

Cell Navigator® Lysosome Staining Kit *NIR Fluorescence*

22652-500Tests 500 Tests
EUR 222
Description: Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

Cell Navigator® Lysosome Staining Kit *Red Fluorescence*

22658-500Tests 500 Tests
EUR 166
Description: Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

Cell Navigator® Lysosome Staining Kit *Green Fluorescence*

22656 500 Tests
EUR 166

Cell Navigator® Lysosome Staining Kit *Blue Fluorescence*

22655-500Tests 500 Tests
EUR 166
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

7-AAD Viability Staining Buffer

abx090617-100tests 100 tests
EUR 260.4

Simport EasyDip Staining Jar Blue

HIS0273 PK6
EUR 117.6

Simport EasyDip Staining Jar Pink

HIS0275 PK6
EUR 117.6

Cell Navigator® Lysosome Staining Kit *Orange Fluorescence*

22657 500 Tests
EUR 166

Cell Navigator® Lysosome Staining Kit *Green Fluorescence*

22656-500Tests 500 Tests
EUR 166
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Simport EasyDip Staining Jar Green

HIS0274 PK6
EUR 117.6

Simport EasyDip Staining Jar White

HIS0276 PK6
EUR 110.4

Simport EasyDip Staining Rack Grey

HIS0278 PK6
EUR 128.4

Cell Navigator® Lysosome Staining Kit *Orange Fluorescence*

22657-500Tests 500 Tests
EUR 166
Description: Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230 50 assays
EUR 622.8
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230-5 5 x 50 assays
EUR 2461.2
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Live-or-Dye UV/448 Fixable Viability Staining Kit (200 assays)

32002 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 405/452 Fixable Viability Staining Kit (200 assays)

32003 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 488/515 Fixable Viability Staining Kit (200 assays)

32004 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 568/583 Fixable Viability Staining Kit (200 assays)

32005 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 594/614 Fixable Viability Staining Kit (200 assays)

32006 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 640/662 Fixable Viability Staining Kit (200 assays)

32007 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 750/777 Fixable Viability Staining Kit (200 assays)

32008 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

Live-or-Dye 405/545 Fixable Viability Staining Kit (200 assays)

32009 1kit
EUR 339.6
Description: Minimum order quantity: 1 unit of 1kit

7-AAD Viability Staining Solution

EXB0026 400 tests
EUR 91.2

Simport EasyDip Staining Jar Yellow

HIS0277 PK6
EUR 110.4

SafePinky DNA Gel Staining Solution

S1000-050 500ml
EUR 157.2

SafePinky DNA Gel Staining Solution

S1000-100 2X500ml
EUR 225.6

SafePinky DNA Gel Staining Solution

S1000-200 4X500ml
EUR 336

Cell Navigator® Mitochondrion Staining Kit *Red Fluorescence*

22668 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *NIR Fluorescence*

22670 500 Assays
EUR 222

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD)

STA-327 96 assays
EUR 790.8
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

MemBriteâ„¢ Fix-ST 650/665 Cell Surface Staining Kit, 500 Reactions

30101 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 650/665 Cell Surface Staining Kit, 100 Reactions

30101-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 667/685 Cell Surface Staining Kit, 500 Reactions

30102 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 667/685 Cell Surface Staining Kit, 100 Reactions

30102-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 681/698 Cell Surface Staining Kit, 500 Reactions

30103 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 681/698 Cell Surface Staining Kit, 100 Reactions

30103-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 755/777 Cell Surface Staining Kit, 500 Reactions

30104 1KIT
EUR 772.8
Description: Minimum order quantity: 1 unit of 1KIT

MemBriteâ„¢ Fix-ST 755/777 Cell Surface Staining Kit, 100 Reactions

30104-T 1KIT
EUR 266.4
Description: Minimum order quantity: 1 unit of 1KIT

Cell Navigator® Mitochondrion Staining Kit *Blue Fluorescence*

22665 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *Red Fluorescence*

22668-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Cell Navigator® Mitochondrion Staining Kit *NIR Fluorescence*

22670-500Assays 500 Assays
EUR 222
Description: Mitochondria are membrane-enclosed organelles found in most eukaryotic cells.

Mouse to Mouse HRP Staining System

MTM003 400 Slides
EUR 433.2

Cell Navigator® Mitochondrion Staining Kit *Green Fluorescence*

22666 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *Orange Fluorescence*

22667 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *Blue Fluorescence*

22665-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Cell Navigator® Mitochondrion Staining Kit *Green Fluorescence*

22666-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Results: The cells formed spheroids well applied, and the addition of Noni in 10, 100 and 200 concentration ng / mL did not produce significant morphological changes. Quantitative values ​​for the survival of the mobile on day 3 showed that the values ​​of absorbance at 450 nm is 0.314 ± 0.013, 0.318 ± 0.008, 0.304 ± 0.000 and 0.300 ± 0.011 for Noni at 0, 10, 100 and 200 concentration ng / mL , each.

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