Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux

Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux

Background: Cerebral ischemia-reperfusion (I / R) injury can cause severe dysfunction, and treatment difficult. It is reported that the nucleotide-binding domain and a leucine-rich repeat protein family of 3 (NLRP3) inflammasome-mediated cell pyroptosis an important part of the I / R injury of the brain and can inhibit the activation of autophagy pyroptosis in some tissue injury. Our previous research found that the protective effect of bone marrow mesenchymal stem cells (BMSCs) in brain I / R injury may be associated with the regulation of autophagy.

Recent research has shown that exosomes secreted from BMSCs (BMSC-Exos) may play an important role in effective biological performance BMSCs and BMSC-Exos protective mechanism associated with the activation of autophagy and remission of inflammation, but have not been reported in studies of injury I / R brain. We aimed to investigate the effects of BMSC-Exos on I / R injury of the brain and determine whether the mechanisms associated with the regulation pyroptosis and autophagic flux.

Methods: PC12 cells were subjected to a shortage of oxygen-glucose / reoxygenation (OGD / R) to induce brain I / R in vitro and cocultured with BMSC-Exos. cell viability was determined by CCK-8 kit and lactate dehydrogenase (LDH) detection. Scanning electron microscopy (SEM), Hoechst 33 342 / propidium iodide (PI) double staining, 2 ‘, 7’-dichlorodihydrofluorescein diacetate assay, immunofluorescence, Western blot and enzyme-linked immunosorbent assay (ELISA) was used to detect cell pyroptosis. Furthermore, transmission electron microscopy (TEM), GFP-RFP-LC3 adenovirus transfection, and Western blot used to detect autophagic flux and its influence on pyroptosis.

Finally, coimmunoprecipitation used to detect the binding interaction between NLRP3 and LC3. Results: BMSC-Exos increased cell viability in OGD / R inhibitory effect of BMSC-Exos on pyroptosis is comparable to NLRP3 inhibitor NLRP3 MCC950 and reversed by excess. Furthermore, BMSC-Exos promoted autophagic flux through AMP-activated kinase (AMPK) / mammalian target of rapamycin on the track, whereas chloroquine, AMPK silencing, and compound C block the inhibitory effects on pyroptosis. Conclusion: BMSC-Exos can protect PC12 cells against injury OGD / R by the weakening of the NLRP3 inflammasome-mediated pyroptosis to promote the AMPK-dependent autophagic flux.

 Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux
Exosomes Secreted From Bone Marrow Mesenchymal Stem Cells Attenuate Oxygen-Glucose Deprivation/Reoxygenation-Induced Pyroptosis in PC12 Cells by Promoting AMPK-Dependent Autophagic Flux

Effect of Morinda citrifolia (Noni) on Cell Viability of Stem Cells and Osteogenesis spheroids

Background and purpose: Morinda citrifolia (Noni) has been widely used in herbal medicine to treat and prevent various diseases. We undertook this study to evaluate the effects of Noni extract on morphology maintenance, increase cellular viability, and improved osteogenesis of stem cell spheroids.

Materials and Methods: We cultured stem cell spheroids made with gingival-derived stem cells in the presence of Noni extract at concentrations of 10, 100 and 200 ng / mL. We do the analysis of changes in cell morphology and cell viability. We did a test alkaline phosphatase activity using a kit and test the mineralization using anthraquinone dye to evaluate osteogenesis of stem cell spheroids with the addition of Noni extract.

?-Galactosidase Staining Kit

AKR-100 1 kit
EUR 432
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

TRAP antibody

22406-100ul 100ul
EUR 390

TRAP Antibody

25505-100ul 100ul
EUR 390

TRAP antibody

70R-12535 100 ul
EUR 457
Description: Affinity purified Rabbit polyclonal TRAP antibody

TRAP-14

5-02017 4 x 5mg Ask for price

Trap 101

B7084-10 10 mg
EUR 373

Trap 101

B7084-50 50 mg
EUR 1363

TRAP -6

ABP-TRA-1 5,0mg vial
EUR 170

TRAP-6

A8675-5 5 mg
EUR 173
Description: Peptide fragment (residues 42-47) of protease-activated receptor 1 (PAR1) that acts as a PAR1 agonist. Stimulates platelet aggregation (EC50 = 0.8 ?M), promotes intracellular Ca2+ mobilization and induces rapid phosphodiesterase 3A (PDE3A) phosphorylation

TRAP-6

H-7764.0500 0.5mg
EUR 48
Description: Sum Formula: C34H56N10O9; CAS# [141136-83-6] net

TRAP-6

H-7764.1000 1.0mg
EUR 60
Description: Sum Formula: C34H56N10O9; CAS# [141136-83-6] net

TRAP-14

H-8105.0001 1.0mg
EUR 151
Description: Sum Formula: C81H118N20O23; CAS# [137339-65-2] net

TRAP-14

H-8105.0005 5.0mg
EUR 515
Description: Sum Formula: C81H118N20O23; CAS# [137339-65-2] net

TRAP-6

H-8365.0005 5.0mg
EUR 273
Description: Sum Formula: C34H56N10O9; CAS# [141136-83-6] net

TRAP-6

H-8365.0025 25.0mg
EUR 998
Description: Sum Formula: C34H56N10O9; CAS# [141136-83-6] net

TRAP-6

HY-P0078 10mM/1mL
EUR 322

TRAP-5

H-1408.0005 5.0mg
EUR 576
Description: Sum Formula: C30H50N8O7; CAS# [141685-53-2] net

TRAP-5

H-1408.0025 25.0mg
EUR 2207
Description: Sum Formula: C30H50N8O7; CAS# [141685-53-2] net

TRAP-7

H-2234.0005 5.0mg
EUR 539
Description: Sum Formula: C39H63N11O10; CAS# [145229-76-1] net

TRAP-7

H-2234.0025 25.0mg
EUR 2061
Description: Sum Formula: C39H63N11O10; CAS# [145229-76-1] net

Rat(TRAP)

QY-E11743 96T
EUR 374

Human TRAP ELISA Kit

EHT0544 96Tests
EUR 521

Bovine TRAP ELISA Kit

EBT0544 96Tests
EUR 521

Chicken TRAP ELISA Kit

ECKT0544 96Tests
EUR 521

Canine TRAP ELISA Kit

ECT0544 96Tests
EUR 521

Goat TRAP ELISA Kit

EGTT0544 96Tests
EUR 521

Mouse TRAP ELISA Kit

ELI-03023m 96 Tests
EUR 865

Sheep TRAP ELISA Kit

EST0544 96Tests
EUR 521

Porcine TRAP ELISA Kit

EPT0544 96Tests
EUR 521

Rat TRAP ELISA Kit

ERT0544 96Tests
EUR 521

Monkey TRAP ELISA Kit

EMKT0544 96Tests
EUR 521

Mouse TRAP ELISA Kit

EMT0544 96Tests
EUR 521

CometAssay Silver Staining Kit

4254-200-K 200 Tests
EUR 325.8

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 370
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

Hoechst 33342 Staining Kit

abx097207-1Kit 1 Kit
EUR 230
  • Shipped within 5-10 working days.

Human Histochemistry Staining Kit

20-abx299039
  • EUR 871.00
  • EUR 1219.00
  • EUR 565.00
  • 150 tests
  • 500 tests
  • 50 tests
  • Shipped within 1 week.

Beta-Galactosidase Staining Kit

K802-250
EUR 294

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 293

Alkaline Phosphatase Staining Kit

K2035-50 50 assays
EUR 379

Guinea Pig TRAP ELISA Kit

EGT0544 96Tests
EUR 521

Scrambled TRAP Fragment

5-01910 4 x 5mg Ask for price

TRAP-14 amide

5-02018 4 x 1mg Ask for price

TRAP-5 amide

5-02019 4 x 5mg Ask for price

[Cit5]-TRAP-5

5-00062 4 x 5mg Ask for price

[Tyr1]-TRAP-7

5-00339 4 x 5mg Ask for price

Polyclonal TRAP Antibody

APR10538G 0.1 mg
EUR 659
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human TRAP . This antibody is tested and proven to work in the following applications:

TRAP-14 Peptide

20-abx266463
  • EUR 551.00
  • EUR 926.00
  • EUR 398.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.

TRAP Conjugated Antibody

C44711 100ul
EUR 397

TRAP Conjugated Antibody

C36331 100ul
EUR 397

TRAP Conjugated Antibody

C38129 100ul
EUR 397

TRAP-14 amide

H-6032.0001 1.0mg
EUR 212
Description: Sum Formula: C81H119N21O22; CAS# [141923-36-6] net

TRAP-14 amide

H-6032.0005 5.0mg
EUR 756
Description: Sum Formula: C81H119N21O22; CAS# [141923-36-6] net

(Tyr1)-TRAP-7

H-1674.0001 1.0mg
EUR 126
Description: Sum Formula: C45H67N11O10; CAS# [149440-16-4] net

(Tyr1)-TRAP-7

H-1674.0005 5.0mg
EUR 418
Description: Sum Formula: C45H67N11O10; CAS# [149440-16-4] net

TRAP-6 amide

H-2936.0005 5.0mg
EUR 115
Description: Sum Formula: C34H57N11O8; CAS# [141923-40-2] net

TRAP-6 amide

H-2936.0025 25.0mg
EUR 368
Description: Sum Formula: C34H57N11O8; CAS# [141923-40-2] net

TRAP-5 amide

H-2938.0025 25.0mg
EUR 539
Description: Sum Formula: C30H51N9O6; CAS# [141923-41-3] net

TRAP-5 amide

H-2938.0100 100.0mg
EUR 1554
Description: Sum Formula: C30H51N9O6; CAS# [141923-41-3] net

CD40 Ligand/TRAP

PR29002 50 ug
EUR 491

Anti-TRAP antibody

STJ190098 200 µl
EUR 197
Description: Unconjugated Mouse monoclonal to TRAP (2H2)

anti-TRAP alpha

YF-PA14790 50 ul
EUR 363
Description: Mouse polyclonal to TRAP alpha

anti-TRAP alpha

YF-PA14791 50 ug
EUR 363
Description: Mouse polyclonal to TRAP alpha

Cell Navigator™ Lysosome Staining Kit *Green Fluorescence with 405 nm Excitation*

22651 500 Tests
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

Cell Navigator™ Mitochondrion Staining Kit *Orange Fluorescence with 405 nm Excitation*

22673 500 Assays
EUR 219
  • R-phrase: R20, R21, R22
  • H-Phrase: H303, H313, H333
  • Symbol for dangerous compounds: Xn
  • UNSPEC Code: 12352200

AAVS1 Safe Harbor Targeting Vector 2.0 - All-Purpose Donor (AAVS1-SA-puro-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE620A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - GOI Knock-in Donor (AAVS1-SA-puro-EF1-MCS), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE622A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

AAVS1 Safe Harbor Targeting Vector 2.0 - Reporter Knock-in Donor (AAVS1-SA-puro-MCS-GFP), Complete Kit with CAS601A-1 (Cas9 SmartNuclease AAVS1-gRNA Targeting Vector) and GE640PR-1 (Junction PCR Primer Mix to confirm AAVS1 integration site)

GE624A-KIT 1 kit
EUR 2132
  • Category: Gene Editing

ExoAb Antibody Kit (CD9, CD63, CD81, Hsp70 antibodies, rabbit anti-human) with goat anti-rabbit HRP secondary antibody

EXOAB-KIT-1 25 ul each
EUR 627
  • Category: Exosomes

Frit Kit

FRIT-KIT 1each
EUR 124
Description: Kit to create frits in capillaries. Includes formamide, Kasil-1, Kasil-1624 and a cleaving tool.

Nerve Terminal Staining Kit V

70034 1SET
EUR 509
Description: Minimum order quantity: 1 unit of 1SET

CaspGLOW Active Caspase Staining Kit

55R-1292 25 assays
EUR 296
Description: Caspase Staining Kit for use activity in the research laboratory

Live/Dead Cell Staining Kit

55R-1434 100 tests
EUR 638
Description: Live/Dead Cell Staining Kit for use in the research laboratory

Blue-Color AP Staining Kit

AP100B-1 50 Assays
EUR 262
  • Category: Stem Cells

Dual- Color AP Staining Kit

AP100D-1 100 Assays
EUR 412
  • Category: Stem Cells

Red-Color AP Staining Kit

AP100R-1 50 Assays
EUR 262
  • Category: Stem Cells

Live-Dead Cell Staining Kit

K501-100
EUR 468

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 238

Red Active Caspase Staining Kit

K2052-100 100 assays
EUR 572

Red Active Caspase Staining Kit

K2052-25 25 assays
EUR 238

Live-Dead Cell Staining Kit

K2081-100 100 staining
EUR 460

DNA Ploidy Analysis Staining Kit

K1439-1
EUR 588

DNA Ploidy Analysis Staining Kit

K1439-5
EUR 2002

Column Packing Kit

PACK-KIT 1pack
EUR 1035
Description: Column packing kit for pressure cells. Includes: HPREG regulator, TBNG10 tubing, CAP-75 capillary, and STRB5X2 stir bar.

TRAP-6 (2-6)

5-02020 4 x 5mg Ask for price

[Phe1, Ser2]-TRAP-6

5-00263 4 x 5mg Ask for price

CD40Ligand/TRAP, human recombinant

4014-10
EUR 137

CD40Ligand/TRAP, human recombinant

4014-1000
EUR 1626

CD40Ligand/TRAP, human recombinant

4014-50
EUR 256

CD40Ligand/TRAP, murine recombinant

4015-100
EUR 642

CD40Ligand/TRAP, murine recombinant

4015-1000
EUR 3149

CD40Ligand/TRAP, murine recombinant

4015-25
EUR 245

CD40Ligand/TRAP, murine recombinant

4015-500
EUR 1953

TRAP-5 amide Peptide

20-abx265852
  • EUR 356.00
  • EUR 537.00
  • EUR 286.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.

Scrambled TRAP Fragment Peptide

20-abx265858
  • EUR 356.00
  • EUR 537.00
  • EUR 286.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.

[Tyr1]-TRAP-7 Peptide

20-abx265889
  • EUR 356.00
  • EUR 537.00
  • EUR 286.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.

[Cit5]-TRAP-5 Peptide

20-abx265945
  • EUR 384.00
  • EUR 592.00
  • EUR 300.00
  • 10 mg
  • 25 mg
  • 5 mg
  • Shipped within 5-10 working days.

(Phe1,Ser2)-TRAP-6

H-5996.0005 5.0mg
EUR 297
Description: Sum Formula: C34H56N10O9; CAS# [374898-11-0] net

(Phe1,Ser2)-TRAP-6

H-5996.0025 25.0mg
EUR 1093
Description: Sum Formula: C34H56N10O9; CAS# [374898-11-0] net

Results: The cells formed spheroids well applied, and the addition of Noni in 10, 100 and 200 concentration ng / mL did not produce significant morphological changes. Quantitative values ​​for the survival of the mobile on day 3 showed that the values ​​of absorbance at 450 nm is 0.314 ± 0.013, 0.318 ± 0.008, 0.304 ± 0.000 and 0.300 ± 0.011 for Noni at 0, 10, 100 and 200 concentration ng / mL , each.

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