Ischaemic preconditioning-induced serum exosomes protect against myocardial ischaemia/reperfusion injury in rats by activating the PI3K/AKT signalling pathway

Ischaemic preconditioning-induced serum exosomes protect against myocardial ischaemia/reperfusion injury in rats by activating the PI3K/AKT signalling pathway

Ischemia / reperfusion (I / R) injury can cause severe arrhythmias and myocardial damage worsens. Exosomes are small membrane vesicles that play a protective role of injury I / R myocardium. This study aims to explore the protective effects of ischemic preconditioning (IPC) induced serum exosomes (IPC-Ex) on the injury I / R myocardium in rats and the underlying mechanism. serum exosomes derived from rats IPC and quantified using bicinchoninic acid assay kit. IPC-Exo (50 mg) injected into the myocardium infarction immediately after the ligation. Rats were randomly divided into Sham, I / R, IPC-Ex + I / R, I / R + LY294002, and I / R + group IPC-Ex + LY294002. hemodynamic parameters measured by physiological recording.

Transthoracic echocardiography is used to detect heart function. Serum levels of creatine kinase-MB isomers, lactate dehydrogenase, aspartate transaminase, tumor necrosis factor-alpha, interleukin (IL) -1β, and IL-10 were detected by enzyme-linked immunosorbent assay. Triphenyl tetrazolium chloride staining was used to measure the size of myocardial infarction. Apoptosis in myocardial tissue was detected by TUNEL staining. Western blotting was used to detect the level of PI3K / AKT and proteins associated with apoptosis.

The results of our study indicate that treatment with IPC-Ex improved heart function and reduce the production of inflammatory factors, cardiomyocyte apoptosis, and myocardial infarct size. In addition, the IPC-Ex treatment promoted protein expression of Bcl-2, p-PI3K and p-AKT but inhibited the caspase-3 and Bax. However, treatment with LY294002 significantly reverse the increase in p-PI3K and p-AKT levels, hemodynamic improvement, and decreased production of inflammatory factor and apoptosis in group I / R + IPC IPC-Exo-Exo-induced. Taken together, our results suggest that the IPC-Ex can reduce the injury I / R via activating PI3K / AKT signaling pathway.

 Ischaemic preconditioning-induced serum exosomes protect against myocardial ischaemia/reperfusion injury in rats by activating the PI3K/AKT signalling pathway
Ischaemic preconditioning-induced serum exosomes protect against myocardial ischaemia/reperfusion injury in rats by activating the PI3K/AKT signalling pathway

Tanshinone IIA ameliorates the development of CAD Through Heart Settings H9c2 Cell Proliferation and Apoptosis by miR-133a-3p / EGFR Axis

Background: Coronary artery disease (CAD) leads to the world of death, serious threat to human health. Tanshinone IIA (Tan IIA), which can be extracted from Danshen, applied in the treatment of cardiovascular and cerebrovascular diseases. MicroRNAs (miRNAs, Mirs) plays an important role in cell proliferation and apoptosis of cells of the cardiovascular system. The purpose of this study was to explore the role of Tan IIA in CAD in vitro and the underlying molecular mechanisms.


Methods: Real-time polymerase chain reaction (RT-PCR) and Western blot used to detect miRNA / mRNA and protein, respectively. target genes miR-133a-3p searched at TargetScan and targeting relationships verified by dual-luciferase reporter assay. Cell proliferation was determined using cells Counting Kit-8 (CCK-8) and Edu labeling. Cell apoptosis was detected by flow cytometry and TUNEL staining.


Results: In this study, lower levels of miR-133a-3p and epidermal growth factor receptor that is higher (EGFR, the target of miR-133a-3p) level found in H2O2-induced cell H9c2. In addition, Tan IIA-regulated miR-133a-3p and downregulated the expression of EGFR. In addition, Tan IIA promoted cell proliferation and reduced apoptosis and increased G0 / G1, which is reversed with inhibitors of miR-133a-3p, while siRNA-EGFR eliminate the effects caused by miR-133a-3p in H2O2-induced cell H9c2.

Cell Navigator® Lysosome Staining Kit *Red Fluorescence*

22658-500Tests 500 Tests
EUR 166
Description: Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

Cell Navigator® Lysosome Staining Kit *Green Fluorescence*

22656 500 Tests
EUR 166

Cell Navigator® Lysosome Staining Kit *Blue Fluorescence*

22655-500Tests 500 Tests
EUR 166
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Cell Navigator® Lysosome Staining Kit *Orange Fluorescence*

22657 500 Tests
EUR 166

Cell Navigator® Lysosome Staining Kit *Green Fluorescence*

22656-500Tests 500 Tests
EUR 166
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Cell Navigator® Lysosome Staining Kit *Orange Fluorescence*

22657-500Tests 500 Tests
EUR 166
Description: Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Red Fluorescence*

22603-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *NIR Fluorescence*

22605-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Cell Navigator® Cell Plasma Membrane Staining Kit *Red Fluorescence*

22681 500 Tests
EUR 222

Cell Navigator® Lysosome Staining Kit *Deep Red Fluorescence*

22659 500 Tests
EUR 166

Cell Navigator® Mitochondrion Staining Kit *Red Fluorescence*

22668 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *NIR Fluorescence*

22670 500 Assays
EUR 222

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Blue Fluorescence*

22600-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.

Cell Navigator® Cell Plasma Membrane Staining Kit *Green Fluorescence*

22682 500 Tests
EUR 222

Cell Navigator® Cell Plasma Membrane Staining Kit *Red Fluorescence*

22681-500Tests 500 Tests
EUR 222
Description: The Cell Navigator® Plasma Membrane Stain Kit provides a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins.

Live or Dead™ Fixable Dead Cell Staining Kit *Red Fluorescence*

22603 200 Tests
EUR 166

Live or Dead™ Fixable Dead Cell Staining Kit *NIR Fluorescence*

22605 200 Tests
EUR 166

Cell Navigator® Lysosome Staining Kit *Deep Red Fluorescence*

22659-500Tests 500 Tests
EUR 166
Description: Lysosomes are cellular organelles which contain acid hydrolase enzymes to break up waste materials and cellular debris.

Cell Navigator® Mitochondrion Staining Kit *Blue Fluorescence*

22665 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *Red Fluorescence*

22668-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Cell Navigator® Mitochondrion Staining Kit *NIR Fluorescence*

22670-500Assays 500 Assays
EUR 222
Description: Mitochondria are membrane-enclosed organelles found in most eukaryotic cells.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Green Fluorescence*

22601-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.

Live or Dead™ Fixable Dead Cell Staining Kit *Blue Fluorescence*

22600 200 Tests
EUR 166

Cell Navigator® Mitochondrion Staining Kit *Green Fluorescence*

22666 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *Orange Fluorescence*

22667 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *Blue Fluorescence*

22665-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Cell Navigator® Mitochondrion Staining Kit *Green Fluorescence*

22666-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Cell Navigator® Cell Plasma Membrane Staining Kit *Orange Fluorescence*

22680 500 Tests
EUR 222

Cell Navigator® Cell Plasma Membrane Staining Kit *Orange Fluorescence*

22680-500Tests 500 Tests
EUR 222
Description: The Cell Navigator® Plasma Membrane Stain Kit provides a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins.

Cell Navigator® Cell Plasma Membrane Staining Kit *Green Fluorescence*

22682-500Tests 500 Tests
EUR 222
Description: The Cell Navigator® Plasma Membrane Stain Kit provides a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Orange Fluorescence*

22602-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.

Live or Dead™ Fixable Dead Cell Staining Kit *Green Fluorescence*

22601 200 Tests
EUR 166

Live or Dead™ Fixable Dead Cell Staining Kit *Orange Fluorescence*

22602 200 Tests
EUR 166

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Deep Red Fluorescence*

22604-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.

Cell Navigator® Mitochondrion Staining Kit *Orange Fluorescence*

22667-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Cell Navigator® TMR Ceramide Golgi Staining Kit *Red Fluorescence*

22752 100 Tests
EUR 318

Live or Dead™ Fixable Dead Cell Staining Kit *Deep Red Fluorescence*

22604 200 Tests
EUR 166

Cell Navigator® Mitochondrion Staining Kit *Deep Red Fluorescence*

22669 500 Assays
EUR 222

Cell Navigator® Mitochondrion Staining Kit *Deep Red Fluorescence*

22669-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Cell Navigator® TMR Ceramide Golgi Staining Kit *Red Fluorescence*

22752-100Tests 100 Tests
EUR 318
Description: The Golgi apparatus is a complex of vesicles and folded membranes within the cytoplasm of most eukaryotic cells, involved in secretion and intracellular transport.

Cell Navigator® NBD Ceramide Golgi Staining Kit *Green Fluorescence*

22750 100 Tests
EUR 222

Cell Navigator® NBD Ceramide Golgi Staining Kit *Green Fluorescence*

22750-100Tests 100 Tests
EUR 222
Description: The Golgi apparatus is a complex of vesicles and folded membranes within the cytoplasm of most eukaryotic cells, involved in secretion and intracellular transport.

Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Red Fluorescence*

22636 100 Tests
EUR 334

Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Blue Fluorescence*

22634 100 Tests
EUR 334

Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Green Fluorescence*

22635 100 Tests
EUR 334

Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Blue Fluorescence*

22634-100Tests 100 Tests
EUR 334
Description: The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae.

Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Red Fluorescence*

22636-100Tests 100 Tests
EUR 334
Description: The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae.

Cell Navigator® Live Cell Endoplasmic Reticulum (ER) Staining Kit *Green Fluorescence*

22635-100Tests 100 Tests
EUR 334
Description: The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae.

Cell Navigator® Lysosome Staining Kit *Green Fluorescence with 405 nm Excitation*

22651 500 Tests
EUR 222

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Blue Fluorescence with 405 nm Excitation*

22500-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Cell Navigator® Lysosome Staining Kit *Green Fluorescence with 405 nm Excitation*

22651-500Tests 500 Tests
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.

Live or Dead™ Fixable Dead Cell Staining Kit *Blue Fluorescence with 405 nm Excitation*

22500 200 Tests
EUR 166

Live or Dead™ Fixable Dead Cell Staining Kit *Green Fluorescence with 405 nm Excitation*

22501 200 Tests
EUR 166

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Green Fluorescence with 405 nm Excitation*

22501-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Orange Fluorescence with 405 nm Excitation*

22502-200Tests 200 Tests
EUR 166
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

Fluorescein Active Caspase Staining Kit

K2047-100 100 assays
EUR 686.4

Fluorescein Active Caspase Staining Kit

K2047-25 25 assays
EUR 285.6

Live or Dead™ Fixable Dead Cell Staining Kit *Orange Fluorescence with 405 nm Excitation*

22502 200 Tests
EUR 166

Cell Navigator® Mitochondrion Staining Kit *Orange Fluorescence with 405 nm Excitation*

22673 500 Assays
EUR 222

ProLiteâ„¢ His-Tag Protein Gel Staining Kit *Green Fluorescence*

18010-10Gels 10 Gels
EUR 412
Description: The polyhistidine tag (His-Tag) is widely used for protein purification, detection, and immobilization.

Cell Navigator® Mitochondrion Staining Kit *Orange Fluorescence with 405 nm Excitation*

22673-500Assays 500 Assays
EUR 222
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc.

Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Red Fluorescence Optimized for Flow Cytometry*

22599-200Tests 200 Tests
EUR 222
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.

ProLite™ His-Tag Protein Gel Staining Kit *Green Fluorescence*

18010 10 Gels
EUR 412

Fluorescein Active Caspase-2 Staining Kit

K2048-100 100 assays
EUR 686.4

Fluorescein Active Caspase-2 Staining Kit

K2048-25 25 assays
EUR 285.6

Fluorescein Active Caspase-3 Staining Kit

K2049-100 100 assays
EUR 686.4

Fluorescein Active Caspase-3 Staining Kit

K2049-25 25 assays
EUR 285.6

Fluorescein Active Caspase-8 Staining Kit

K2050-100 100 assays
EUR 686.4

Fluorescein Active Caspase-8 Staining Kit

K2050-25 25 assays
EUR 285.6

Fluorescein Active Caspase-9 Staining Kit

K2051-100 100 assays
EUR 686.4

Fluorescein Active Caspase-9 Staining Kit

K2051-25 25 assays
EUR 285.6

Live or Dead™ Fixable Dead Cell Staining Kit *Red Fluorescence Optimized for Flow Cytometry*

22599 200 Tests
EUR 222

Fluorescein Active Caspase-12 Staining Kit

K2046-100 100 assays
EUR 686.4

Fluorescein Active Caspase-12 Staining Kit

K2046-25 25 assays
EUR 285.6

MycoLightâ„¢ Rapid Fluorescence Gram-Positive Bacteria Staining Kit

22415-100Tests 100 Tests
EUR 334
Description: AAT Bioquest's MycoLight™ Rapid Fluorescence Gram-Positive Bacteria Staining Kit provides a novel one-step fluorescence assay for the determination of gram sign in living bacteria.

MycoLight™ Rapid Fluorescence Gram-Positive Bacteria Staining Kit

22415 100 Tests
EUR 334

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase Staining Kit

K180-25 each
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-3 Staining Kit

K183-25 each
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-100 each
EUR 627.6

CaspGLOW? Fluorescein Active Caspase-2 Staining Kit

K182-25 each
EUR 280.8

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-9 Staining Kit

K189-25 each
EUR 288

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-100 each
EUR 660

CaspGLOW? Fluorescein Active Caspase-8 Staining Kit

K188-25 each
EUR 288

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-100 each
EUR 627.6

CaspGLOW? Fluorescein Active Caspase-12 Staining Kit

K172-25 each
EUR 280.8

?-Galactosidase Staining Kit

AKR-100 1 kit
EUR 518.4
Description: This simple assay measures the transfection efficiency of the LacZ gene using a simple staining protocol.

StemTAG Alkaline Phosphatase Staining and Activity Assay Kit, Fluorometric

CBA-308 2 x 100 assays
EUR 846
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230 50 assays
EUR 622.8
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

Cellular Senescence Detection Kit (SA-?-Gal Staining)

CBA-230-5 5 x 50 assays
EUR 2461.2
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD)

STA-327 96 assays
EUR 790.8
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

StemTAG Alkaline Phosphatase Staining Kit (Red)

CBA-300 100 assays
EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP)

STA-329 96 assays
EUR 790.8
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of 6-4PP structures in DNA by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

StemTAG Alkaline Phosphatase Staining Kit (Purple)

CBA-306 100 assays
EUR 553.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.

Maximo-Gel juice fluorescent stain

310012L 10x10 ml
EUR 1904.4

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD), Trial Size

STA-327-T 32 assays
EUR 421.2
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

OxiSelect DNA Double-Strand Break (DSB) Staining Kit

STA-321 100 assays
EUR 790.8
Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.

OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP), Trial Size

STA-329-T 32 assays
EUR 421.2
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of 6-4PP structures in DNA by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.

OxiSelect DNA Double Strand Break (DSB) Staining Kit, Trial Size

STA-321-T 20 assays
EUR 421.2
Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.

includes fluorescent DNA stain and tracking dyes

DM101-0105 5x600µl
EUR 206.4

includes fluorescent DNA stain and tracking dyes

DM110-0105 5x600µl
EUR 206.4

Beta Galactosidase Staining Kit

55R-1541 250 assays
EUR 444
Description: Assay Kit for detection of Beta Galactosidase in the research laboratory

OxiSelect Cellular Antioxidant Assay Kit (Green Fluorescence)

STA-349 192 assays
EUR 644.4
Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells.  Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested.  After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator.  The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF.  The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.

StemTAG Alkaline Phosphatase Staining and Activity Assay Kit, Colorimetric

CBA-302 2 x 100 assays
EUR 769.2
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.

Beta-Galactosidase Staining Kit

K802-250 each
EUR 352.8

Beta-Galactosidase Staining Kit

K2182-250 250 assays
EUR 351.6

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012 10 ml
EUR 222

Maximo-Gel juice fluorescent Protein-Gel Gel stain

310012-L 10x10ml
EUR 1904.4

Fluorescent

FPAK-3058--9K 9X1 mL
EUR 675.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particle Kit

FB-2552-6K 6x1 mL
EUR 554.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particle Kit

FB-3558-6K 6x1 mL
EUR 554.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particle Kit

FA-2552-6K 6x1 mL
EUR 554.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particle Kit

FA-3558-6K 6x1 mL
EUR 554.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particle Kit

FX-3552-6K 6x1 mL
EUR 432
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particle Kit

FX-3552-9K 9X1 mL
EUR 675.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

STA-347 96 assays
EUR 734.4
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)

STA-347-5 5 x 96 assays
EUR 2913.6
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

STA-342 96 assays
EUR 762
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence)

STA-342-5 5 x 96 assays
EUR 3058.8
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size

STA-347-T 20 assays
EUR 400.8
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.

Nano Fluorescent Size Standard Kit

NFPPS-52-4K 4X1 mL
EUR 426
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

ADAM17 Fluorescent Assay Kit

78000 96 rxns.
EUR 465
Description: The ADAM17 Fluorescent Assay Kit is provided in a convenient 96-well format, with purified ADAM17, ADAM Fluorogenic Substrate, and ADAM assay buffer for 96 enzyme reactions. The key to the ADAM17 Fluorescent Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM17 reactions. A sample containing ADAM17 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=358±10 nm, λem=455±10 nm) is measured using a plate reader.

TMPRSS2 Fluorescent Assay Kit

78083 96 rxns.
EUR 1205
Description: The key to the TMPRSS2 Fluorescent Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for TMPRSS2 reactions. A sample containing TMPRSS2 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=383nm, λem=455nm) is measured using a plate reader. Camostat is supplied as a protease inhibitor control.

HDM Fluorescent Kit (2 Plate)

K010-F1 2x96 well plates
EUR 466

Fluorescent Particle Array Kit

FPAK-4058--10K 10 mL
EUR 675.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

OxiSelect Intracellular ROS Assay Kit (Green Fluorescence), Trial Size

STA-342-T 20 assays
EUR 414
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.

Biotin Fluorescent Particle Kit

TFA-3558-6K 6x1 mL
EUR 554.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Biotin Fluorescent Particle Kit

TFB-3558-6K 6x1 mL
EUR 554.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particle

FP-3045-5 5 mL
EUR 303.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Carboxyl Fluorescent Particle Kit

CFA-2552-6K 6x1 mL
EUR 554.4
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.

Carboxyl Fluorescent Particle Kit

CFA-2558-6K 6x1 mL
EUR 554.4
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.

Carboxyl Fluorescent Particle Kit

CFA-3558-6K 6x1 mL
EUR 554.4
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.

Carboxyl Fluorescent Particle Kit

CFB-2552-6K 6x1 mL
EUR 554.4
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.

Carboxyl Fluorescent Particle Kit

CFB-2558-6K 6x1 mL
EUR 554.4
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.

Carboxyl Fluorescent Particle Kit

CFB-3558-6K 6x1 mL
EUR 554.4
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.

Fluorescent Particles

FH-10040-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-10052-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-10056-10 10 mL
EUR 1022.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-10056-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-10062-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-10074-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-10078-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-2040-2 2 mL
EUR 230.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-2045-2 2 mL
EUR 230.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-2052-2 2 mL
EUR 230.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-2056-2 2 mL
EUR 230.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-2060-2 2 mL
EUR 261.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-2062-2 2 mL
EUR 230.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-2070-2 2 mL
EUR 230.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-3056-10 10 mL
EUR 802.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-3056-2 2 mL
EUR 237.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FH-5056-2 2 mL
EUR 237.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-00552-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-00556-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-00558-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-00562-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-00570-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0245-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0252-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0256-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0257-2 2 mL
EUR 237.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0262-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0270-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0545-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0552-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0556-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0557-2 2 mL
EUR 237.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0558-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0562-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0570-2 2 mL
EUR 218.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0842-2 2 mL
EUR 225.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0845-2 2 mL
EUR 225.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0852-2 2 mL
EUR 225.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0856-2 2 mL
EUR 225.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0857-2 2 mL
EUR 242.4
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0858-2 2 mL
EUR 225.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0862-2 2 mL
EUR 225.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0868-2 2 mL
EUR 225.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0870-2 2 mL
EUR 225.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-0878-2 2 mL
EUR 225.6
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-10040-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-10045-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-10052-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-10056-10 10 mL
EUR 888
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-10056-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-10056-2A 2X1 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-10062-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.

Fluorescent Particles

FP-10066-2 2 mL
EUR 310.8
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.


Conclusion: Tan IIA reversed H2O2-induced reduction of cell proliferation, induction of cell apoptosis and G0 / G1 arrest in the cell reduction H9c2 by miR-133a-3p / EGFR axis. The findings suggest a molecular basis Tan IIA potential in treating patients with CAD.

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