Ischaemic preconditioning-induced serum exosomes protect against myocardial ischaemia/reperfusion injury in rats by activating the PI3K/AKT signalling pathway
Ischemia / reperfusion (I / R) injury can cause severe arrhythmias and myocardial damage worsens. Exosomes are small membrane vesicles that play a protective role of injury I / R myocardium. This study aims to explore the protective effects of ischemic preconditioning (IPC) induced serum exosomes (IPC-Ex) on the injury I / R myocardium in rats and the underlying mechanism. serum exosomes derived from rats IPC and quantified using bicinchoninic acid assay kit. IPC-Exo (50 mg) injected into the myocardium infarction immediately after the ligation. Rats were randomly divided into Sham, I / R, IPC-Ex + I / R, I / R + LY294002, and I / R + group IPC-Ex + LY294002. hemodynamic parameters measured by physiological recording.
Transthoracic echocardiography is used to detect heart function. Serum levels of creatine kinase-MB isomers, lactate dehydrogenase, aspartate transaminase, tumor necrosis factor-alpha, interleukin (IL) -1β, and IL-10 were detected by enzyme-linked immunosorbent assay. Triphenyl tetrazolium chloride staining was used to measure the size of myocardial infarction. Apoptosis in myocardial tissue was detected by TUNEL staining. Western blotting was used to detect the level of PI3K / AKT and proteins associated with apoptosis.
The results of our study indicate that treatment with IPC-Ex improved heart function and reduce the production of inflammatory factors, cardiomyocyte apoptosis, and myocardial infarct size. In addition, the IPC-Ex treatment promoted protein expression of Bcl-2, p-PI3K and p-AKT but inhibited the caspase-3 and Bax. However, treatment with LY294002 significantly reverse the increase in p-PI3K and p-AKT levels, hemodynamic improvement, and decreased production of inflammatory factor and apoptosis in group I / R + IPC IPC-Exo-Exo-induced. Taken together, our results suggest that the IPC-Ex can reduce the injury I / R via activating PI3K / AKT signaling pathway.
Ischaemic preconditioning-induced serum exosomes protect against myocardial ischaemia/reperfusion injury in rats by activating the PI3K/AKT signalling pathway
Tanshinone IIA ameliorates the development of CAD Through Heart Settings H9c2 Cell Proliferation and Apoptosis by miR-133a-3p / EGFR Axis
Background: Coronary artery disease (CAD) leads to the world of death, serious threat to human health. Tanshinone IIA (Tan IIA), which can be extracted from Danshen, applied in the treatment of cardiovascular and cerebrovascular diseases. MicroRNAs (miRNAs, Mirs) plays an important role in cell proliferation and apoptosis of cells of the cardiovascular system. The purpose of this study was to explore the role of Tan IIA in CAD in vitro and the underlying molecular mechanisms.
Methods: Real-time polymerase chain reaction (RT-PCR) and Western blot used to detect miRNA / mRNA and protein, respectively. target genes miR-133a-3p searched at TargetScan and targeting relationships verified by dual-luciferase reporter assay. Cell proliferation was determined using cells Counting Kit-8 (CCK-8) and Edu labeling. Cell apoptosis was detected by flow cytometry and TUNEL staining.
Results: In this study, lower levels of miR-133a-3p and epidermal growth factor receptor that is higher (EGFR, the target of miR-133a-3p) level found in H2O2-induced cell H9c2. In addition, Tan IIA-regulated miR-133a-3p and downregulated the expression of EGFR. In addition, Tan IIA promoted cell proliferation and reduced apoptosis and increased G0 / G1, which is reversed with inhibitors of miR-133a-3p, while siRNA-EGFR eliminate the effects caused by miR-133a-3p in H2O2-induced cell H9c2.
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.
Live or Deadâ„¢ Fixable Dead Cell Staining Kit *NIR Fluorescence*
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.
Description: The Cell Navigator® Plasma Membrane Stain Kit provides a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins.
Live or Dead™ Fixable Dead Cell Staining Kit *Red Fluorescence*
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.
Live or Dead™ Fixable Dead Cell Staining Kit *Blue Fluorescence*
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.
Description: The Cell Navigator® Plasma Membrane Stain Kit provides a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins.
Description: The Cell Navigator® Plasma Membrane Stain Kit provides a fast and uniform labeling of the plasma membrane without the cell-type differences exhibited by lectins.
Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Orange Fluorescence*
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.
Live or Dead™ Fixable Dead Cell Staining Kit *Green Fluorescence*
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cell functions.
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.
Description: The Golgi apparatus is a complex of vesicles and folded membranes within the cytoplasm of most eukaryotic cells, involved in secretion and intracellular transport.
Description: The Golgi apparatus is a complex of vesicles and folded membranes within the cytoplasm of most eukaryotic cells, involved in secretion and intracellular transport.
Description: The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae.
Description: The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae.
Description: The endoplasmic reticulum (ER) is a type of organelle in the cells of eukaryotic organisms that forms an interconnected network of flattened, membrane-enclosed sacs or tube-like structures known as cisternae.
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria and nuclei etc.
Live or Dead™ Fixable Dead Cell Staining Kit *Blue Fluorescence with 405 nm Excitation*
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.
Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Orange Fluorescence with 405 nm Excitation*
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.
Description: Our Cell Navigator® fluorescence imaging kits are a set of fluorescence imaging tools for labeling sub-cellular organelles such as membranes, lysosomes, mitochondria, nuclei, etc.
Live or Deadâ„¢ Fixable Dead Cell Staining Kit *Red Fluorescence Optimized for Flow Cytometry*
Description: Our Live or Dead™ Fixable Dead Cell Staining Kits are a set of tools for labeling cells for fluorescence microscopic investigations of cellular functions.
ProLite™ His-Tag Protein Gel Staining Kit *Green Fluorescence*
Description: AAT Bioquest's MycoLight™ Rapid Fluorescence Gram-Positive Bacteria Staining Kit provides a novel one-step fluorescence assay for the determination of gram sign in living bacteria.
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.
Description: Our Cellular Senescence Staining Kit provides an efficient method to visualize Senescence Associated (SA) ß-galactosidase. SA-ß-Gal catalyzes the hydrolysis of X-gal, which produces a blue color in senescent cells. Visualize results with a standard light microscope.
OxiSelect Cellular UV-Induced DNA Damage Staining Kit (CPD)
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.
OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP)
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of 6-4PP structures in DNA by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Staining Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining.
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of cyclobutane pyrimidine dimers (CPD) by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-CPD antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
OxiSelect DNA Double-Strand Break (DSB) Staining Kit
Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.
OxiSelect Cellular UV-Induced DNA Damage Staining Kit (6-4PP), Trial Size
Description: Our OxiSelect Cellular UV-Induced DNA Damage Staining Kit measures the formation of 6-4PP structures in DNA by immunofluorescence. Cells are first seeded in a 96-well tissue culture plate. Wells are then UV irradiated to induce DNA damage. After fixation and denaturation, cells containing the DNA lesions are probed with an anti-6-4PP antibody, followed by a FITC conjugated secondary antibody. The unbound secondary antibody is removed during a wash step, and stained cells can then be visualized with a fluorescence microscope.
OxiSelect DNA Double Strand Break (DSB) Staining Kit, Trial Size
Description: Double-strand breaks (DSB) in DNA are among the most dangerous types of DNA damage occuring within cells. One of the earliest cellular responses to double-strand breaks is the phosphorylation of a histone variant, H2AX, at the sites of DNA damage. Within seconds Ser139 is phosphorylated when DSBs are induced in mammalian cells. Phosphorylation of this serine residue causes chromatin condensation and appears to play a critical role in the recruitment of repair or damage-signaling factors to the DNA damage sites. The OxiSelect DNA Double-Strand Break Staining Kit provides an easy-to-use method for detecting the presence of DSBs in cells cultured in microtiter plates. Double strand breaks can be detected in just a few hours by immunofluorescence staining of the phosphorylated histone H2AX.
Description: The OxiSelect Cellular Antioxidant Assay Kit is a cell-based assay for measuring the activity of an exogenous antioxidant compound within adherent cells. Cells are first cultured in a 96-well black fluorescence cell culture plate until confluent. Then the cells are pre-incubated with a cell-permeable DCFH-DA fluorescence probe dye and the bioflavonoid Quercetin, or the antioxidant sample being tested. After a brief incubation, the cells are washed, and the reaction started by adding the Free Radical Initiator. The Free Radical Initiator creates free radicals that convert the probe to highly fluorescent DCF. The Quercetin inhibits the formation of free radicals, and thus DCF formation, in a concentration dependent manner.
StemTAG Alkaline Phosphatase Staining and Activity Assay Kit, Colorimetric
Description: Alkaline Phosphatase (AP) is a widely used marker for both mouse and human embryonic stem cells (ES) and embryonic germ cells (EG). Our StemTAG Alkaline Phosphatase kits provide an efficient system for monitoring cell differentiation or undifferentiation using the AP marker. The StemTAG Alkaline Phosphatase Activity Assay Combo Kits provide reagents for monitoring alkaline phosphatase activity via immunocytochemistry staining as well as in a 96-well plate with either colorimetric or fluorescence detection.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
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OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.
OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence)
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.
OxiSelect In Vitro ROS/RNS Assay Kit (Green Fluorescence), Trial Size
Description: The OxiSelect In Vitro ROS/RNS Assay provides a sensitive method to detect total reactive oxygen species (ROS) plus reactive nitrogen species (RNS) in a wide variety of sample types. This assay employs a proprietary fluorogenic probe, DCFH-DiOxyQ; the probe is primed with a dequenching reagent to the highly reactive DCFH form. In the presence of ROS and RNS, the DCFH is rapidly oxidized to the highly fluorescent DCF.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: The ADAM17 Fluorescent Assay Kit is provided in a convenient 96-well format, with purified ADAM17, ADAM Fluorogenic Substrate, and ADAM assay buffer for 96 enzyme reactions. The key to the ADAM17 Fluorescent Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for ADAM17 reactions. A sample containing ADAM17 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=358±10 nm, λem=455±10 nm) is measured using a plate reader.
Description: The key to the TMPRSS2 Fluorescent Assay Kit is the fluorogenic substrate. Using this kit, only one simple step on a microtiter plate is required for TMPRSS2 reactions. A sample containing TMPRSS2 is incubated in a reaction mixture with the fluorogenic substrate and fluorescence (λex=383nm, λem=455nm) is measured using a plate reader. Camostat is supplied as a protease inhibitor control.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: The OxiSelect ROS Assay Kit is a cell-based assay for measuring hydroxyl, peroxyl, and other reactive oxygen species activity within a cell. The assay employs the cell-permeable fluorogenic probe DCFH-DA, which diffuses into cells and is deacetylcated by cellular esterases into the non-fluorescent DCFH (Figure 1). In the presence of ROS, DCFH is rapidly oxidized to highly fluorescent DCF. Fluorescence is read on a standard fluorometric plate reader.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
Description: Please reffer to the technical data sheet for more detail information for this item. Our dedicated team would be happy to assist you via live chat, email or phone.
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Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.
Description: Gentaur distributes carboxyl, functionalized surface groups used for covalent coupling.If you need more technical information please contact our dedicated team via live chat, phone or email.
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Conclusion: Tan IIA reversed H2O2-induced reduction of cell proliferation, induction of cell apoptosis and G0 / G1 arrest in the cell reduction H9c2 by miR-133a-3p / EGFR axis. The findings suggest a molecular basis Tan IIA potential in treating patients with CAD.